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Amycolatopsis acididurans sp. nov., isolated from peat swamp forest soil in Thailand

The Journal of Antibiotics volume 74, pages 199–205 (2021)Cite this article

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Abstract

A polyphasic approach was used to describe strain K13G38T, a novel actinomycete isolated from peat swamp forest soil collected from Surat Thani Province, Thailand. The 16S rRNA gene phylogenetic analysis indicated that the strain belonged to the genus Amycolatopsis and showed the highest sequence similarities to both Amycolatopsis acidiphila JCM 30562T and Amycolatopsis bartoniae DSM 45807T (96.8% sequence similarity). Furthermore, strain K13G38T, which formed extensively branched substrate and aerial mycelia, exhibited chemotaxonomical characteristics of the genus Amycolatopsis which included phospholipid pattern type II and cell-wall chemotype IV. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, two unidentified phospholipids, and an unidentified aminolipid. MK-9(H4) was a predominant menaquinone of the organism. The major cellular fatty acids were iso-C16:0, anteiso-C17:0, and C16:0. The genomic DNA size of strain K13G38T was 8.5 Mbp with 69.5 mol% G+C content. On the basis of phenotypic characteristics, overall genomic relatedness index and phylogenetic distinctiveness, strain K13G38T represents a novel species of the genus Amycolatopsis, for which the name A. acididurans sp. nov. is proposed. The type strain is K13G38T (=TBRC 12507T = NBRC 114553T).

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Acknowledgements

This research was supported by Center of Excellence on Biodiversity (BDC), Office of Higher Education Commission (Project Code BDC-PG1-160003) and supported in part by the Graduate Program Scholarship from The Graduate School, Kasetsart University to Teo Wee Fei Aaron. The authors are grateful to Professor Dr. Savitree Limtong of Kasetsart University, the director of the research program. This work was partially supported by UGSAS-GU via the “Microbiology Laboratory Station for IC - GU12” at Kasetsart University.

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  1. Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand

    Wee Fei Aaron Teo, Kenika Lipun, Nantana Srisuk & Kannika Duangmal

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  1. Wee Fei Aaron Teo
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Correspondence to Kannika Duangmal.

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Teo, W.F.A., Lipun, K., Srisuk, N. et al. Amycolatopsis acididurans sp. nov., isolated from peat swamp forest soil in Thailand. J Antibiot 74, 199–205 (2021). https://doi.org/10.1038/s41429-020-00382-2

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