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Identification and Fine Mapping of a Major QTL, TT1-2, That Plays Significant Roles in Regulating Heat Tolerance in Rice

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Abstract

Global warming threatens many aspects of human life, including a reduction in crop yields, and breeding heat-tolerant crops is a fundamental way to help address this challenge. As food for more than half of the global population, rice (Oryza sativa) has always been a popular research material in plant science. Breeding heat-tolerant rice using genes affording thermotolerance is a fundamental way to address this challenge. In this study, a major QTL, TT1-2, was found to regulate heat tolerance in rice; this QTL was controlled by a single dominant gene. Using F2:3 populations, we narrowed TT1-2 to a 26.0-kb region containing three putative genes, one of which encodes an α2 subunit of the 26S proteasome. This gene was considered the TT1-2 candidate, and the TT1 gene involved in rice heat tolerance was present at this locus. Further analysis showed that the amino acid sequence of the TT1-2 gene had one amino acid difference: arginine (R)-99 was changed to histidine (H), which leads to the formation of a normal alpha-helix. Moreover, through marker-assisted selection and conserved breeding selection, we developed a new male-sterile line, Zhehang 10A, which had a high outcrossing rate, good quality, and strong heat tolerance. We then used Zhehang 10A as the female parent and the restorer line Fuhui 1586 as the male parent and bred a new hybrid, Zhehangyou 1586, which showed high yield, good quality, and strong heat tolerance.

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Acknowledgments

We thank American Journal Experts for providing professional editing services.

Funding

This work was supported by the National Keypoint Research and Invention Program of the Thirteenth (No. 2017YFD0300102), Special Fund for Agro-scientific Research in the Public Interest of Fujian Province (No. 2020R11010016-3), Youth Technology Innovation Team of Fujian Academy of Agricultural Sciences (No. STIT2017-3-3), Fujian Provincial Natural Science Foundation of China (No. 2019J01011040), and General Project of Fujian Academy of Agricultural Sciences (No. A2017-13).

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Authors and Affiliations

  1. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Chuan Yan & Xiaofu Hong

  2. Rice Research Institute, Fujian High Quality Rice Research & Development Center, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China

    Dewei Yang

  3. Collage of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Guanping Zhan

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  1. Chuan Yan
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  2. Guanping Zhan
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  3. Xiaofu Hong
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  4. Dewei Yang
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Contributions

CY drafted the manuscript. CY, DY, GZ and HX contributed to the data analysis. DY and CY participated in the design of the study and the interpretation of the results and wrote and edited the manuscript.

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Correspondence to Dewei Yang.

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Key Message

A major QTL, TT1-2, was found to regulate heat tolerance in rice, and a single amino acid difference in the TT1-2 gene could affect the formation of a normal alpha-helix. A new hybrid combination, Zhehangyou 1586, was bred using a developed molecular marker linked to TT1-2.

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Yan, C., Zhan, G., Hong, X. et al. Identification and Fine Mapping of a Major QTL, TT1-2, That Plays Significant Roles in Regulating Heat Tolerance in Rice. Plant Mol Biol Rep 39, 376–385 (2021). https://doi.org/10.1007/s11105-020-01256-5

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  • DOI: https://doi.org/10.1007/s11105-020-01256-5

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