Abstract
Key message
78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism.
Abstract
Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and development, signal transduction, and responses to environmental stresses. In this study, 78 moso bamboo (Phyllostachys edulis) HD-Zip genes were investigated and classified into four subfamilies (HD-Zip I-IV). Additionally, Phehdz1 (HD-Zip I gene) was isolated and confirmed to be highly expressed in the roots. A quantitative real-time PCR analysis indicated Phehdz1 expression was significantly induced by drought, high salinity, and abscisic acid (ABA). A transient expression assay proved that Phehdz1 was localized in the nucleus of tobacco cells. Moreover, it could bind to the core region encoded by the H-box sequence (CAATAATTG) in yeast. In response to mannitol treatments, the Phehdz1-overexpressing transgenic rice had a higher germination rate and longer shoots than the wild-type controls. Moreover, Phehdz1-overexpressing rice plants had a higher survival rate as well as higher relative water and proline contents, but a lower malondialdehyde content, than the WT plants after a 30% polyethylene glycol 6000 treatment. Accordingly, the overexpression of Phehdz1 enhances the drought tolerance of transgenic rice. Many of the differentially expressed genes identified by a transcriptome analysis are involved in MAPK signal transduction and the biosynthesis of secondary metabolites. Thus, the overexpression of Phehdz1 enhances the drought stress tolerance of transgenic rice, while also potentially modulating the expression of metabolism-related genes.
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We thank LiwenBianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.
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This work was supported by the National Natural Science Foundation of China (31670672) and the Graduate Innovation Fund from Anhui Agricultural University (2020ysj-55).
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299_2020_2625_MOESM1_ESM.tif
Figure S1. Amino acid and structural analysis of Phehdz1. A. Schematic diagram of amino acid translation of Phehdz1 gene. B. Schematic diagram of Phehdz1 CDS domain and. C. Schematic diagram of the promoter of Phehdz1 gene. (TIF 266 kb)
299_2020_2625_MOESM2_ESM.tif
Figure S2. GUS staining and PCR gel electrophoresis. A. The GUS staining of WT and the overexpression lines of Phehdz1. B. Phehdz1 PCR gel electrophoresis in the genome of the overexpression lines. (TIF 2946 kb)
299_2020_2625_MOESM3_ESM.tif
Figure S3. A. Go classification statistics of differentially expressed genes. B. The relative expression levels of six stress/ABA-responsive genes. (TIF 832 kb)
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Gao, Y., Liu, H., Zhang, K. et al. A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice. Plant Cell Rep 40, 187–204 (2021). https://doi.org/10.1007/s00299-020-02625-w
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DOI: https://doi.org/10.1007/s00299-020-02625-w