Skip to main content

Advertisement

Log in

A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice

  • Original Article
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Key message

78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism.

Abstract

Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and development, signal transduction, and responses to environmental stresses. In this study, 78 moso bamboo (Phyllostachys edulis) HD-Zip genes were investigated and classified into four subfamilies (HD-Zip I-IV). Additionally, Phehdz1 (HD-Zip I gene) was isolated and confirmed to be highly expressed in the roots. A quantitative real-time PCR analysis indicated Phehdz1 expression was significantly induced by drought, high salinity, and abscisic acid (ABA). A transient expression assay proved that Phehdz1 was localized in the nucleus of tobacco cells. Moreover, it could bind to the core region encoded by the H-box sequence (CAATAATTG) in yeast. In response to mannitol treatments, the Phehdz1-overexpressing transgenic rice had a higher germination rate and longer shoots than the wild-type controls. Moreover, Phehdz1-overexpressing rice plants had a higher survival rate as well as higher relative water and proline contents, but a lower malondialdehyde content, than the WT plants after a 30% polyethylene glycol 6000 treatment. Accordingly, the overexpression of Phehdz1 enhances the drought tolerance of transgenic rice. Many of the differentially expressed genes identified by a transcriptome analysis are involved in MAPK signal transduction and the biosynthesis of secondary metabolites. Thus, the overexpression of Phehdz1 enhances the drought stress tolerance of transgenic rice, while also potentially modulating the expression of metabolism-related genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

Download references

Acknowledgements

We thank LiwenBianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (31670672) and the Graduate Innovation Fund from Anhui Agricultural University (2020ysj-55).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Min Wu or Yan Xiang.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Additional information

Communicated by Neal Stewart.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

299_2020_2625_MOESM1_ESM.tif

Figure S1. Amino acid and structural analysis of Phehdz1. A. Schematic diagram of amino acid translation of Phehdz1 gene. B. Schematic diagram of Phehdz1 CDS domain and. C. Schematic diagram of the promoter of Phehdz1 gene. (TIF 266 kb)

299_2020_2625_MOESM2_ESM.tif

Figure S2. GUS staining and PCR gel electrophoresis. A. The GUS staining of WT and the overexpression lines of Phehdz1. B. Phehdz1 PCR gel electrophoresis in the genome of the overexpression lines. (TIF 2946 kb)

299_2020_2625_MOESM3_ESM.tif

Figure S3. A. Go classification statistics of differentially expressed genes. B. The relative expression levels of six stress/ABA-responsive genes. (TIF 832 kb)

Figure S4. The alignment of amino acid sequences of four subfamily HD-Zip gene members. (TIF 7940 kb)

Table S1. The primer sequences of related experiments. (XLSX 15 kb)

Table S2. The details of 20 motifs. (XLSX 18 kb)

Table S3. The detailed information of 78 HD-Zip genes in moso bamboo. (XLSX 15 kb)

Table S4. The duplication types, Ka, Ks and the Ka/Ks values of paralogous gene pairs (Phe-Phe). (XLSX 20 kb)

Table S5. The Ka, Ks and the Ka/Ks values of orthologous gene pairs (Bd-Phe, Os-Phe, Sb-Phe, Zm-Phe). (XLSX 42 kb)

Table S6. The information of differentially expressed genes in different pathways. (XLS 1008 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gao, Y., Liu, H., Zhang, K. et al. A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice. Plant Cell Rep 40, 187–204 (2021). https://doi.org/10.1007/s00299-020-02625-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-020-02625-w

Keywords

Navigation