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QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers

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Abstract

Fourteen cucumber lines were tested for genetic homozygosity and performed pairwise comparison to identify a pair with the highest DNA polymorphic level. Cucumber accessions CSL0067 and CSL0139 were selected to generate 315 F2 populations. The genetic linkage map based on 66 polymorphic SSR markers was constructed. It composed of eight linkage groups (LGs) spanning 474.4 cM. Downy mildew disease reaction was evaluated in cotyledons, first and second true leaf on 7, 10, and 14 day after inoculation. The results showed that downy mildew resistance was controlled by multiple recessive genes. The susceptible to resistant ratio of F2 progenies fit 9:7 susceptible/resistant segregation types corresponding to duplicate recessive epistasis. Fourteen QTLs were detected. The phenotypic variance ranged from 5.0 to 12.5%, while LOD values ranged from 3.538 to 9.165. Two major QTLs and two QTL hotspots were identified. Moreover, the additive effects data explained that these QTL reduced downy mildew susceptibility.

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Acknowledgements

This research was funded by National Center for Genetic Engineering and Biotechnology (BIOTEC) and the Faculty of Science, Kasetsart University. The greenhouse for phenotypic analysis was screened at Chia Tai Company Limited, Chiang Mai, Thailand.

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Author notes

  1. Pawinee Innark and Hadsaya Panyanitikoon contributed equally to this work.

Authors and Affiliations

  1. Faculty of Science and Technology, Dhonburi Rajabhat University, Bangkok, 10600, Thailand

    PAWINEE INNARK

  2. Department of Genetics, Faculty of Science, Kasetsart University, Bangkhen Campus, Bangkok, 10900, Thailand

    HADSAYA PANYANITIKOON, SOMPID SAMIPAK & CHATCHAWAN JANTASURIYARAT

  3. Lampang Agricultural Research and Training Centre, Rajamangala University of Technology Lanna, Lampang, 52000, Thailand

    CHANULAK KHANOBDEE

  4. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASTNAR, NRU-KU), Kasetsart University, Bangkok, 10900, Thailand

    SOMPID SAMIPAK & CHATCHAWAN JANTASURIYARAT

Authors
  1. PAWINEE INNARK
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  2. HADSAYA PANYANITIKOON
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  3. CHANULAK KHANOBDEE
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  4. SOMPID SAMIPAK
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  5. CHATCHAWAN JANTASURIYARAT
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Correspondence to CHATCHAWAN JANTASURIYARAT.

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Corresponding editor: H. A. Ranganath

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INNARK, P., PANYANITIKOON, H., KHANOBDEE, C. et al. QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers. J Genet 99, 81 (2020). https://doi.org/10.1007/s12041-020-01242-6

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  • DOI: https://doi.org/10.1007/s12041-020-01242-6

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