Abstract
Sheath blight disease of rice causes substantial crop losses and resistance sources are rare. A moderately resistant genotype CR 1014 was identified and hybridized with highly susceptible genotype Swarna-Sub1. In the F2 and F2:3 generations, three QTLs (qShB-1.1, qShB-1.2 and qShB-1.3) were mapped in chromosome-1. In F5 generation of the same cross and F4 generation of an alternative mapping population (Tapaswini/CR 1014), only the major QTL qShB-1.1 was recorded consistently with high LOD score (> 5.0). This stable QTL was co-localized with qShB1 reported earlier from Oryza nivara. A typical leucine rich repeat (LRR) motif containing gene (LOC_Os01g65650) and a chitin-inducible gibberellin-responsive protein coding non-LRR gene (LOC_Os01g65900) located within qShB-1.1 with high expression levels in leaf and shoot were predicted as putative candidate genes among others. Nearly 27.8% reduction in relative lesion height was recorded among several near isogenic lines of Swarna-Sub1 carrying the QTL region from CR 1014.
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Acknowledgements
The authors acknowledge the support of Indian Council of Agricultural Research for funding the research through project no. EAP-201 (Incentivizing Research in Agriculture). The financial and administrative support from Director, ICAR-National Rice Research Institute, Cuttack through institute project 1.3 (2017-20) is also gratefully acknowledged. Authors are also thankful to Dr. Nitiprasad Jambhulkar, Scientist (Agricultural Statistics), ICAR-National Rice Research Institute for his support in statistical analysis.
Funding
This study was financially supported by Indian Council of Agricultural Research funding for Project “Incentivizing research in agriculture” (EAP-201) along with institutional fund for Project 1.3 (2017-20) from ICAR-National Rice Research Institute.
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AB, PS, RS, SS, PRS, MLB and AK carried out field works, net house screening and genotyping; MKK conceptualized and designed the experiments, developed mapping populations, analyzed data, arranged resources and supervised the research; MC supported in designing of the experiments and genotyping, analyzed data and prepared draft manuscript; AKM and SL supervised the disease screening and phenotyping; SR and LB carried out the bioinformatics analyses; KAM and TPMA critically reviewed the data and manuscript; AB, PS and MC contributed equally for the work. All authors read and approved the final manuscript.
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Bal, A., Samal, P., Chakraborti, M. et al. Stable quantitative trait locus (QTL) for sheath blight resistance from rice cultivar CR 1014. Euphytica 216, 182 (2020). https://doi.org/10.1007/s10681-020-02702-x
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DOI: https://doi.org/10.1007/s10681-020-02702-x