Abstract
Salicylic acid (SA) induces the mechanism of the plant defence and plays a role in plant -pathogen interactions. Following the nematode infection, effects of SA treatment on plant weights and the expression of pathogen related gene have not been fully understood. The present study was aimed to determine the effects of SA treatment on the expression Pathogenesis related 1 gene (PR1 gene) and alteration on plant parameters in tomatoes (Solanum lycopersicum). Plant seedlings were dripped within the SA solution. The expression of PR1 gene achieved using RT-PCR at 1, 3, 7, 14, 21-days post infection (dpi) with Meloidogyne incognita. Upregulation of PR1 gene was determined in early (1 dpi) and late (21 dpi). SA treatment and nematode infection altered plant parameters. SA treatment increased the plant defence mechanisms in tomato against Meloidogyne incognita.
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REFERENCES
Bozbuga, R., Dasgan, H.Y., Akhoundnejad, Y., Imren, M., Toktay, H., and Kasapoglu, E.B., Identification of common bean (Phaseolus vulgaris) genotypes having resistance against root knot nematode, Meloidogyne incognita,Legume Res., 2015, vol. 38, no. 5, pp. 669–674.
Bozbuga, R., Lilley, J.L., Knox, J.P., and Urwin, P.E., Host-specific signatures of the cell wall changes induced by the plant parasitic nematode, Meloidogyne incognita,Sci. Rep., 2018, vol. 8, p. 17302.
Bozbuga, R., Dasgan, H.Y., Akhoundnejad, Y., Imren, M., Gunay, O., and Toktay, H., Effect of mi gene and nematode resistance on tomato genotypes using molecular and screening assay, Cytol. Genet., 2020, vol. 54, pp. 154–164.
Jones, J.D.G. and Dangl, J.L., The plant immune system, Nature, 2006, vol. 444, pp. 323–329.
van Loon, L.C., Pierpoint, W.S., Boller, T., and Conejero, V., Recommendations for naming plant pathogenesis-related proteins, Plant Mol. Biol. Rep., 1994, vol. 12, pp. 245–264.
Yan, S. and Dong, X., Perception of the plant immune signal salicylic acid, Curr. Opin. Plant Biol., 2014, vol. 20, pp. 64–68.
Shi, Z., Maximova, S.N., Liu, Y., Verica, J., and Guiltinan, M.J., Functional analysis of the Theobroma cacao NPR1 gene in Arabidopsis, BMC Plant Biol., 2010, vol. 10, p. 248.
Ginzinger, D.G., Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream, Exp. Hematol., 2002, vol. 30, pp. 503–512.
Kavroulakis, N., Ntougias, S., Zervakis, G.I., Ehaliotis, C., Haralampidis, K., and Papadopoulou, K.K., Role of ethylene in the protection of tomato plants against soil-borne fungal pathogens conferred by an endophytic Fusarium solani strain, J. Exp. Bot., 2007, vol. 58, pp. 3853–3864.
Chinnapandi, B., Bucki, P., and Braun Miyara, S., SLWRKY45, nematode-responsive tomato WRKY gene, enhances susceptibility to the root knot nematode; M. javanica infection, Plant Signal. Behav., 2017, vol. 12, e1356530.
van Loon, L.C., Rep, M., and Pieterse, C.M.J., Significance of inducible defense-related proteins in infected plants, Annu. Rev. Phytopathol., 2006, vol. 44, pp. 135–162.
Lavrova, V.V., Zinovieva, S.V., Udalova, Z.V., and Matveeva, E.M., Expression of PR genes in tomato tissues infected by nematode Meloidogyne incognita (Kofoid et White, 1919) Chitwood, 1949, Dokl. Biochem. Biophys., 2017, vol. 476, pp. 306–309.
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Bozbuga, R. Expressions of Pathogenesis related 1 (PR1) Gene in Solanum lycopersicum and Influence of Salicylic Acid Exposures on Host-Meloidogyne incognita Interactions. Dokl Biochem Biophys 494, 266–269 (2020). https://doi.org/10.1134/S1607672920050038
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DOI: https://doi.org/10.1134/S1607672920050038