Abstract
Only a limited number of techniques are available for assessing the effect of different coating materials on cell adherence to screws. In this study, we describe a simple and inexpensive method for evaluation of cell adhesion on irregular surfaces such as the surgical or implant screws. For this purpose, we prepared semi-submerged screws in the petri dishes using agar. Using BSA- or HA-coated screws, we tested whether BSA or HA could improve cell adherence when used as coating materials. Agar-coated screws were used as internal control. Then the “ratio of cell adherence” was calculated by subtracting the reference RCA value obtained from the agar coated screws (internal control). When compared to that of the non-coated screws both the HA- and BSA-coating improved cell adherence on the screws by 2.34 and 2.72 fold respectively. Similarly, MTT assay data revealed that the metabolic capacities of cells on HA- or BSA-coated screws were improved by 2.36 and 2.86 fold respectively. These findings suggest that this protocol can be used for comparing the ability of cells to attach on irregular surfaces such as dental or orthopedic screws and assessing their viability.
Similar content being viewed by others
Data and/or code availability
All the cell lines, raw experimental data, data on statistical analysis and/or detailed explanations regarding Materials and methods are available from the corresponding author on reasonable request.
References
García AJ, Gallant ND (2003) Stick and grip: measurement systems and quantitative analyses of integrin-mediated cell adhesion strength. Cell Biochem Biophys 39:61–73. https://doi.org/10.1385/CBB:39:1:61
Horváthy DB, Vácz G, Szabõ T, Szigyártõ IC, Torõ I, Vámos B, Hornyák I, Renner K, Klára T, Szabõ BT, Dobõ-Nagy C, Doros A, Lacza Z (2016) Serum albumin coating of demineralized bone matrix results in stronger new bone formation. J Biomed Mater Res Part B Appl Biomater 104:126–132. https://doi.org/10.1002/jbm.b.33359
Khalili AA, Ahmad MR (2015) A review of cell adhesion studies for biomedical and biological applications. Int J Mol Sci 16:18150. https://doi.org/10.3390/ijms160818149
Osman RB, Swain MV (2015) A critical review of dental implant materials with an emphasis on titanium versus Zirconia. Materials 8:932–958. https://doi.org/10.3390/ma8030932
Rosa AL, Beloti MM, Van Noort R (2003) Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography. Dent Mater 19:768–772. https://doi.org/10.1016/S0109-5641(03)00024-1
Schulz MC, Korn P, Stadlinger B, Range U, Möller S, Becher J, Schnabelrauch M, Mai R, Scharnweber D, Eckelt U, Hintze V (2014) Coating with artificial matrices from collagen and sulfated hyaluronan influences the osseointegration of dental implants. J Mater Sci Mater Med 25:247–258. https://doi.org/10.1007/s10856-013-5066-3
Scudiere DA, Shoemaker RH, Paul KD, Monks A, Tierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines1. Cancer Res 48:4827–4833
Strober W (2015) Trypan blue exclusion test of cell viability. Curr Protoc Immunol 111:A3.B.1–A3.B.3. https://doi.org/10.1002/0471142735.ima03bs111
Tanaka N, Moriguchi H, Sato A, Kawai T, Shimba K, Jimbo Y, Tanaka Y (2016) Microcasting with agarose gel via degassed polydimethylsiloxane molds for repellency-guided cell patterning. RSC Adv 6:54754–54762. https://doi.org/10.1039/C6RA11563B
Acknowledgements
This project would have been impossible without the support of Assoc. Prof. Dr. Cenk KIG.
Funding
Not applicable.
Author information
Authors and Affiliations
Contributions
Gundogan G. I. carried out the experiments. Gundogan G. I. designed the model of the study and provided literature search. Gundogan G. I. contributed to processing, analysis, and collection of data. The manuscript was written by Gundogan G. I.
Corresponding author
Ethics declarations
Conflicts of interest
The authors declare that she has no competing interests.
Consent for publication
Not applicable.
Ethics approval
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Gundogan, G.İ. A simple and inexpensive method for evaluation of in vitro cell adhesion on screws. Cytotechnology 72, 847–854 (2020). https://doi.org/10.1007/s10616-020-00428-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-020-00428-1