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Robust and Self-healable Antibiofilm Multilayer Coatings

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Abstract

The infection induced by implantation of biomedical materials may result from the biofilm formation after bacteria attachment. Hence, the antibiofilm surface coating represents a novel technique to improve the antibacterial activity of biomedical materials. The traditional antibiofilm surface coatings exhibited some disadvantages and provided a limited service life. In this work, we used polyethyleneimine grafted 3-maleimidopropionic acid (PEIM) and poly(acrylic acid) grafted 2-furfurylamine (PAAF) to achieve robust and self-healable crosslinked multilayer coatings, employing Layer-by-Layer (LbL) self-assembly technique and Diels-Alder reaction. Then, thiol-terminated poly((3-acrylamidopropyl) trimethylammonium chloride) (PAMPTMA-SH) was grafted onto the crosslinked multilayer coating by thiol-ene click reaction to form a novel multilayer coating (PEIM/PAAF)10-PAMPTMA. We found that this coating showed robust and self-healable activity, and significantly inhibited the bacterial growth and biofilm formation after infection with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by in vitro and in vivo assays for 120 h. In addition, the multilayer coating did not induce significant hemolysis or affect the cell viability of red blood cells. In vivo studies also showed that (PEIM/PAAF)10-PAMPTMA coating efficiently blocked the infiltration of inflammatory cells and gene expression in the mouse skin challenged with E. coli or S. aureus. Taken together, these results showed that the prepared multilayer coating exhibited strong antibiofilm activity and provided a new strategy for the application of highly efficient antibiofilm surface coating of biomedical materials.

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Acknowledgments

This work was financially supported by the Natural Science Foundation of Jiangsu Province (No. BK20180963) and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 19KJD430001).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, 213164, China

    Chao Zhou, Jun-Tao Zhou, Cheng-Ju Sheng, Yan Pan & Xiao-Jia Huang

  2. Department of Chemical and Biomolecular Engineering, National University of Singapore, 117585, Singapore, Singapore

    Dicky Pranantyo

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  1. Chao Zhou
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  2. Jun-Tao Zhou
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  3. Cheng-Ju Sheng
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  4. Dicky Pranantyo
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Correspondence to Chao Zhou.

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Zhou, C., Zhou, JT., Sheng, CJ. et al. Robust and Self-healable Antibiofilm Multilayer Coatings. Chin J Polym Sci 39, 425–440 (2021). https://doi.org/10.1007/s10118-021-2513-3

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