Abstract
The influence of putrescine (Put), spermidine (Spd), and spermine (Spm) on somatic embryogenesis (SE) and plant regeneration of three indica rice cultivars (ASD16, IR64, and ADT43) were evaluated since polyamines (PAs) are reported to play vital roles in SE and plant regeneration. Significant increases in the regeneration potential of the rice cultivars upon polyamine treatments were observed. Put (1.0 mM) induced the highest regeneration frequencies, somatic embryos, and shoot numbers. PAs influenced the fresh weights of the suspension cultures. Spd was found to be detrimental to cultivar ADT43 towards embryogeneicity, regeneration, and somatic embryo induction. Among the cultivars, ASD16 was found to respond superiorly to the polyamine treatment. Experiments with long-term cultures of the rice revealed that Put and Spm enhanced the regeneration potential, and the highest frequencies were recorded for ASD16 and IR64. Results indicate that PAs can be effectively used as growth modulators to achieve success in in vitro tissue and genetic manipulation studies with elite recalcitrant indica rice cultivars.
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References
Altman A, Nadel BL, Falash Z, Levin N (1990) Antibiotic resistant mutants of Solanum nigrum. In: Nijkamp HJJ, VanMonPlas LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer Academic Publishers, Amsterdam, pp 454–459. https://doi.org/10.1007/978-94-009-2103-0
Astarita LV, Handro W, Floh EI (2003) Changes in polyamines content associated with zygotic embryogenesis in the Brazilian pine, Araucaria angustifolia (Bert.) O. Ktze. Braz J Bot 26:163–168. https://doi.org/10.1590/S0100-84042003000200003
Bajaj S, Rajam MV (1995) Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell Rep 14:717–720. https://doi.org/10.1007/BF00232654
Bajaj S, Rajam MV (1996) Polyamine accumulation and near-loss of morphogenesis in long-term callus cultures of rice: restoration of plant regeneration by manipulation of cellular polyamine levels. Plant Physiol 112:1343–1348. https://doi.org/10.1104/pp.112.3.1343
Chong-Perez B, Reyes M, Rojas L, Ocana B, Perez B, Kosky RG, Angenon G (2012) Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in banana cv. ‘Dwarf Cavendish’ (Musa AAA): effect of spermidine on transformation efficiency. Plant Cell, Tissue Organ Cult 111:79–90. https://doi.org/10.1007/s11240-012-0174-1
Chu CC (1978) The N6 medium and its applications to anther culture of cereal crops. In: Proceedings of symposium on plant tissue culture. Science Press, pp 43–50
Datta SK, Peterhans A, Datta K, Potrykus I (1990) Genetically engineered fertile indica-rice recovered from protoplasts. Bio/Technology 8:736. https://doi.org/10.1038/nbt0890-736
Dievart A, Perin C, Hirsch J, Bettembourg M, Lanau N, Artus F, Pereira S (2016) The phenome analysis of mutant alleles in leucine-rich repeat receptor-like kinase genes in rice reveals new potential targets for stress tolerant cereals. Plant Sci 242:240–249. https://doi.org/10.1016/j.plantsci.2015.06.019
Erland LAE, Mahmoud SS (2014) An efficient method for regeneration of lavandin (Lavandula × intermedia cv. ‘Grosso’). Vitro Cell Dev Biol Plant 50:646–654. https://doi.org/10.1007/s11627-014-9614-4
Faure O, Mengoli M, Nougarede A, Bagni N (1991) Polyamine pattern and biosynthesis in zygotic and somatic embryo stages of Vitis vinifera. J Plant Physiol 138:545–549. https://doi.org/10.1016/S0176-1617(11)80238-5
Ganesan M, Jayabalan N (2006) Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2). Indian J Exp Biol 44:506–513
Handa AK, Mattoo AK (2010) Differential and functional interactions emphasize the multiple roles of polyamines in plants. Plant Physiol Biochem 48:540–546. https://doi.org/10.1016/j.plaphy.2010.02.009
Hiei Y, Komari T, Kubo T (1997) Transformation of rice mediated by Agrobacterium tumefaciens. Plant Mol Biol 35:205–218. https://doi.org/10.1023/A:1005847615493
Kakkar RK, Nagar PK, Ahuja PS, Rai VK (2000) Polyamines and plant morphogenesis. Biol Plant 43:1–11. https://doi.org/10.1023/A:1026582308902
Mengoli M, Bagni N, Luccarini G, Nutironchi V, Serafini-Fracassini D (1989) Daucus carota cell cultures: polyamines and effect of polyamine biosynthesis inhibitors in preembryogenic phase and different embryo stages. J Plant Physiol 143:389–394. https://doi.org/10.1016/S0176-1617(89)80001-X
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Mustafavi SH, Badi HN, Sękara A, Mehrafarin A, Janda T, Ghorbanpour M, Rafiee H (2018) Polyamines and their possible mechanisms involved in plant physiological processes and elicitation of secondary metabolites. Acta Physiol Plant 40:102. https://doi.org/10.1007/s11738-018-2671-2
Palavan-Unsal N, Cotuk A, Kalac-Martini Y (2002) Difluoromethyl ornithine inhibits the growth of fungus Macrophomina phaseoli. Turk J Bot 26:43–45
Rajam MV (1993) Polyamine biosynthesis inhibitors: new protectants against fungal plant diseases. Curr Sci 461–469. https://www.jstor.org/stable/24095648
Rajam MV, Shoeb F, Yadav JS (1997) Polyamines as modulators of plant regeneration in tissue cultures. In: Srivastava PS (ed) Plant tissue culture and molecular biology: applications and prospects. Narosa Publishing House, New Delhi, pp 620–641
Sathish S, Venkatesh R, Safia N, Sathishkumar R (2018) Studies on growth dynamics of embryogenic cell suspension cultures of commercially important indica rice cultivars ASD16 and Pusa basmati. 3 Biotech 8:194. https://doi.org/10.1007/s13205-018-1213-3
Shen HJ, Galston AW (1985) Correlations between polyamine ratios and growth patterns in seedling roots. Plant Growth Regul 3:53–363. https://doi.org/10.1007/BF00117592
Shoeb F, Yadav JS, Bajaj S, Rajam MV (2001) Polyamines as biomarkers for plant regeneration capacity: improvement of regeneration by modulation of polyamine metabolism in different genotypes of Indica rice. Plant Sci 160:1229–1235. https://doi.org/10.1016/S0168-9452(01)00375-2
Sundararajan S, Sivaraman B, Rajendran V, Ramalingam S (2017) Tissue culture and Agrobacterium-mediated genetic transformation studies in four commercially important indica rice cultivars. J Crop Sci Biotechnol 20:175–183. https://doi.org/10.1007/s12892-017-0045-0
Sundararajan S, Rajendran V, Nayeem S, Ramalingam S (2020) Physicochemical factors modulate regeneration and Agrobacterium-mediated genetic transformation of recalcitrant indica rice cultivars-ASD16 and IR64. Biocatal Agric Biotechnol 24:101519
Takeda T, Hayakawa F, Oe K, Matsuoka H (2002) Effects of exogenous polyamines on embryogenic carrot cells. Biochem Eng J 12:21–28. https://doi.org/10.1016/S1369-703X(02)00037-2
Talapatra S, Goshal N, Raychaudhuri SS (2013) Molecular characterization, modeling and expression analysis of a somatic embryogenesis receptor kinase (SERK) gene in Momordica charantia L. during somatic embryogenesis. Plant Cell Tissue Organ Cult 116:271–283. https://doi.org/10.1007/s11240-013-0401-4
Tang W, Newton RJ, Outhavong V (2004) Exogenously added polyamines recover browning tissues into normal callus cultures and improve plant regeneration in pine. Physiol Plant 122:386–395. https://doi.org/10.1111/j.1399-3054.2004.00406.x
Thiruvengadam M, Parveen N, Chung IM (2012) Evaluated antioxidant potential in Momordica charantia L. through in direct organogenesis. Afr J Biotechnol 11:33
Tiburcio AF, Alcázar R (2018) Potential applications of polyamines in agriculture and plant biotechnology. In: Alcázar R, Tiburcio A (eds) Polyamines. Humana Press, New York, pp 489–508. https://doi.org/10.1007/978-1-4939-7398-9_40
Visarada KBRS, Sailaja M, Sarma NP (2002) Effect of callus induction media on morphology of embryogenic calli in rice genotypes. Biol Plant 45:495–502. https://doi.org/10.1023/A:1022323221513
Wimalasekera R, Tebartz F, Scherer GFE (2011) Polyamines, polyamine oxidase and nitric oxide in development, abiotic and biotic stresses. Plant Sci 181:593–603. https://doi.org/10.1016/j.plantsci.2011.04.002
Zeng YH, Zahng YP, Xiang J, Hui WU, Chen HZ, Zhang YK, Zhu DF (2016) Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity, endogenous hormones and ultrastructure of indica–japonica hybrid rice seedlings. J Int Agric 15:295–308. https://doi.org/10.1016/S2095-3119(15)61051-6
Acknowledgements
Author Sathish. S acknowledges Indian Council for Medical Research (ICMR), New Delhi, India (No. 3/1/2/102/2018-Nut.) for fellowship support. Hari Priya Sivakumar thanks Department of Science and Technology, Innovation in Science Pursuit for Inspired Research (DST-INSPIRE) for fellowship support (IF80901). We would also like to thank University Grants Commission-Special Assistance Programme (UGC-SAP) and Department of Science and Technology—Fund for Improvement of Science & Technology infrastructure in universities & higher educational institutions (DST-FIST) for the financial support to carry out this research.
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Sundararajan, S., Sivakumar, H.P., Nayeem, S. et al. Influence of exogenous polyamines on somatic embryogenesis and regeneration of fresh and long-term cultures of three elite indica rice cultivars. CEREAL RESEARCH COMMUNICATIONS 49, 245–253 (2021). https://doi.org/10.1007/s42976-020-00098-x
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DOI: https://doi.org/10.1007/s42976-020-00098-x