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Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil

  • Bacterial and Fungal Pathogenesis - Research Paper
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Abstract

Salmonella Enteritidis has caused, since the 1980s, a sustained epidemic of human infections in many countries. This study analyzed S. Enteritidis strains isolated before and after the epidemic period in Brazil regarding their capacities to survive to acid, oxidative, and high-temperature stresses, and capacity to grow in egg albumen. Moreover, the ability to invade human epithelial cells (Caco-2) and to survive inside human (U937) and chicken (HD11) macrophages was checked. Post-epidemic strains showed a better ability to survive after 10 min under acid stress at 37 °C (P ≤ 0.05). However, both groups of strains showed similar ability to survive after 1 h under acid stress at 37 °C and at 42 °C independently of the time of exposure. Similar ability was verified in both groups of strains regarding oxidative stress, growth in egg albumen, high-temperature stress, invasion to Caco-2 cells, and invasion and survival in macrophages. In conclusion, post-epidemic S. Enteritidis strains showed a better ability to survive under the acid stress found in the stomach, which might be an advantage to reach the intestine and colonize chickens and humans. However, both groups of strains did not differ significantly in the majority of the phenotypic tests analyzed in this study.

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Funding

This study was financed by São Paulo Research Foundation (FAPESP) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. During the course of this work, Dr. Fabio Campioni was supported by postdoctoral fellowships from Sao Paulo Research Foundation - FAPESP (Proc. 2013/25191-3). Dr. Juliana Pfrimer Falcão received a grant from FAPESP (Proc. 2016/24716-3) and a productivity fellowship from CNPQ (Grant 304399/2018-3).

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Authors and Affiliations

  1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP, Av. do Café, s/n. Bloco S – Sala 41, Ribeirão Preto, SP, 14040-903, Brazil

    Fábio Campioni, Carolina Nogueira Gomes & Juliana Pfrimer Falcão

  2. Laboratório de Enterobactérias, Fundação Oswaldo Cruz – FIOCRUZ, Av. Brasil, 4365, Pavilhão Rocha Lima, Rio de Janeiro, RJ, 21040-900, Brazil

    Dália dos Prazeres Rodrigues

  3. Laboratório de Microbiologia de Alimentos, Instituto Adolfo Lutz – Centro de Laboratórios Regionais - Ribeirão Preto VI, Rua Minas 877, Ribeirão Preto, SP, 14085-410, Brazil

    Alzira Maria Morato Bergamini

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  1. Fábio Campioni
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  2. Carolina Nogueira Gomes
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Correspondence to Juliana Pfrimer Falcão.

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Campioni, F., Gomes, C.N., Rodrigues, D.d.P. et al. Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil. Braz J Microbiol 52, 173–183 (2021). https://doi.org/10.1007/s42770-020-00392-0

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