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Panton-Valentine leukocidin (PVL) isoforms among Staphylococcus aureus lineages isolated from hospitals in Rio de Janeiro, Brazil

  • Bacterial and Fungal Pathogenesis - Short Communication
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Abstract

Panton-Valentine leukocidin (PVL) is a Staphylococcus aureus virulence factor codified by lukSF-PV genes. Single-nucleotide polymorphisms (SNPs) at lukSF-PV genes can lead to two PVL sequence variants (R and H) generating different PVL isoforms. This study analyzed lukSF-PV genes SNPs among four different clonal lineages (STs/CC 1, 5, 8, and 30) of nine S. aureus isolated at Brazilian hospitals. The sequenced products showed SNPs at seven sites (positions 121, 470, 527, 663, 856, 1396, and 1729), leading to non-synonymous substitutions in all isolates investigated. Our findings showed new R and H isoforms variants in S. aureus isolated in Brazil and suggest a possible relationship between H2b isoform and the ST30/CC30 lineage.

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Funding

This study was supported by Brazilian grants from Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento Pessoal de Nível Superior - Brasil (CAPES) – Finance Code 001.

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Authors and Affiliations

  1. Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, Brazil

    Raiane Cardoso Chamon

  2. Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Raiane Cardoso Chamon, Tamara Lopes Rocha de Oliveira, Rosana Barreto Rocha Ferreira & Kátia Regina Netto dos Santos

  3. Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Lilian Oliveira Moreira

Authors
  1. Raiane Cardoso Chamon
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  2. Tamara Lopes Rocha de Oliveira
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  3. Rosana Barreto Rocha Ferreira
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  4. Lilian Oliveira Moreira
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  5. Kátia Regina Netto dos Santos
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Contributions

Conceptualization: Raiane Cardoso Chamon; Methodology: Raiane Cardoso Chamon, Tamara Lopes Rocha de Oliveira, and Rosana Barreto Rocha Ferreira; Formal analysis and investigation: Raiane Cardoso Chamon; Writing - original draft preparation: Raiane Cardoso Chamon; Writing - review and editing: Lilian Oliveira Moreira and Kátia Regina Netto dos Santos; Funding acquisition: Kátia Regina Netto dos Santos; Resources: Kátia Regina Netto dos Santos; Supervision: Lilian Oliveira Moreira and Kátia Regina Netto dos Santos.

Corresponding author

Correspondence to Kátia Regina Netto dos Santos.

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The authors declare that they have no conflict of interest.

Ethics approval

The isolates used at the present study belong to the “Laboratório de Infecção Hospitalar” collection, a reference laboratory at Universidade Federal do Rio de Janeiro.

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Responsible Editor: Mariana X Byndloss.

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Chamon, R.C., de Oliveira, T.L.R., Ferreira, R.B.R. et al. Panton-Valentine leukocidin (PVL) isoforms among Staphylococcus aureus lineages isolated from hospitals in Rio de Janeiro, Brazil. Braz J Microbiol 51, 1509–1513 (2020). https://doi.org/10.1007/s42770-020-00386-y

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  • DOI: https://doi.org/10.1007/s42770-020-00386-y

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