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Identification, characterisation and expression analysis of MADS-box genes in sweetpotato wild relative Ipomoea trifida

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Abstract

MADS-box transcription factors (TFs) participate in various biological processes, including stress response, plant growth and development. The storage root of sweetpotato (Ipomoea batatas) is an important staple food crop in Asian and African areas, and leafy sweetpotato is a popular and healthy vegetable in China. However, the function of the MADS genes remains largely unknown in sweetpotato and the other Ipomoea species. In this study, we carried out a genome-wide gene family analysis of MADS in Ipomoea trifida which is currently considered as the most potential ancestor of sweetpotato. A total of 37 ItfMADS genes that encode 42 proteins were identified. The evolutionary relationship was analysed for MADS proteins in 37 species. According to the tree topology of phylogeny, the ItfMADS genes are classified into Mα, Mβ, Mγ, MIKCC and MIKC* groups. Gene structure analysis revealed that the majority of ItfMADS in the same clade displayed similar exon–intron distribution. Furthermore, the expression patterns of ItfMADS genes were investigated through RNA-seq data and confirmed by qRT-PCR. The expression of ItfMADS genes varied among different tissues, and induced or repressed under different abiotic stresses, thereby suggesting their potential functions under abiotic stresses. Our findings lay a solid foundation for further elucidating the ItfMADS function and promoting sweetpotato molecular breeding.

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Acknowledgements

This work was supported jointly by the projects of the National Key R&D Program of China (2018YFD1000705, 2018YFD1000700), the National Natural Science Foundation of China (Grant No. 31701481), the Natural Science Foundation of Jiangsu Higher Education Institutions of China (19KJA510010), and the Key R&D Program of of Xuzhou-Modern Agriculture (KC20039).

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  1. Panpan Zhu and Tingting Dong contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, Jiangsu Province, China

    Panpan Zhu, Tingting Dong & Tao Xu

  2. Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, South Korea

    Panpan Zhu & Hunseung Kang

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  1. Panpan Zhu
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  2. Tingting Dong
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Correspondence to Tao Xu or Hunseung Kang.

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Zhu, P., Dong, T., Xu, T. et al. Identification, characterisation and expression analysis of MADS-box genes in sweetpotato wild relative Ipomoea trifida. Acta Physiol Plant 42, 163 (2020). https://doi.org/10.1007/s11738-020-03153-6

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