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Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae

  • Systems and Synthetic Microbiology and Bioinformatics
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Abstract

Adipic Acid (AA) is a valued platform chemical compound, which can be used as a precursor of nylon-6,6. Due to the generation of an enormous amount of nitric oxide metabolites and the growing depletion of oil resources as a result of AA production from a mixture of cyclohexanol and cyclohexanone, the microbial methods for synthesizing AA have attracted significant attention. Of the several AA-producing pathways, the reverse adipate degradation pathway in Thermobifida fusca (Tfu RADP) is reported to be the most efficient, which has been confirmed in Escherichia coli. In this study, the heterologous Tfu RADP was constructed for producing AA in S. cerevisiae by co-expressing genes of Tfu_0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576, and Tfu_2576. The AA titer combined with biomass, cofactors and other by-products was all determined after fermentation. During batch fermentation in a shake flask, the maximum AA titer was 3.83 mg/L, while the titer increased to 10.09 mg/L during fed-batch fermentation in a 5-L bioreactor after fermentation modification.

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Acknowledgments

This work was funded by grants from the National Key R&D Program of China (2019YFA0905502), the National Natural Science Foundation of China (21877053, 31601564), the Natural Science Foundation of Jiangsu Province (BK20181345), the Fundamental Research Funds for the Central Universities (JUSRP51705A), the Open Foundation of Jiangsu Key Laboratory of Industrial Biotechnology (KLIB-KF201807), and the Postgraduate Research & Practice Innovation Program of Jiangsu Provence (KYCX17_1424).

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Authors and Affiliations

  1. National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, P. R. China

    Xi Zhang, Yunying Zhao & Yu Deng

  2. China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University, Beijing, 100048, P. R. China

    Yingli Liu & Jing Wang

  3. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, P. R. China

    Xi Zhang & Yu Deng

  4. Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, P. R. China

    Yunying Zhao & Yu Deng

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  1. Xi Zhang
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  2. Yingli Liu
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  3. Jing Wang
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  4. Yunying Zhao
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Correspondence to Yunying Zhao or Yu Deng.

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All authors declare that they have no conflicts of interest with this manuscript.

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Zhang, X., Liu, Y., Wang, J. et al. Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae. J Microbiol. 58, 1065–1075 (2020). https://doi.org/10.1007/s12275-020-0261-7

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