Abstract
This study demonstrates the susceptibility of the Escherichia coli and Bacillus subtilis planktonic bacteria to inactivation by shock acoustic waves that arise from the rapid formation and collapse of vapor bubbles in a medium locally heated to its boiling point. Local heating of the medium occurred due to heat release through the relaxation of highly excited electronic states of exogenous molecules of organic dyes. Dye molecules were excited by nanosecond laser pulses. Highly excited electronic states were formed as a result of stepwise absorption of two quanta of laser radiation. The dependence of the efficiency of microorganism inactivation on the dye concentration, excitation power density, and the distance from the shock wave source was studied.
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REFERENCES
J. O’Neill, Review on Antimicrobial Resistance. Tackling a Global Health Crisis: Initial Steps (Wellcome Trust, London, 2015).
S. D. Zakharov and A. B. Ivanov, Kvant. Elektron. 29 (3), 192 (1999).
A. A. Krasnovsky, Jr., Biophysics (Moscow) 49 (2), 289 (2004).
S. N. Letuta, S. N. Pashkevich, A. T. Ishemgulov, et al., J. Photochem. Photobiol. B 163, 232 (2016).
M. Scholz and R. Dědic, in Singlet Oxygen: Applications in Biosciences and Nanosciences, Ed. by S. Nonell and C. Flors (Roy. Soc. Chem., 2016), Vol. 2, pp. 63–81.
A. A. Krasnovsky, Jr., Biochemistry 72 (10), 1065 (2007).
M. R. Hamblin, Curr. Opin. Microbiol. 33, 67 (2016).
J. W. Costerton, P. S. Stewart, and E. P. Greenberg, Science 284, 1318 (1999).
G. A. O’Toole, H. B. Kaplan, and R. Kolter, Annu. Rev. Microbiol. 54, 49, (2000).
A. P. Veduta, M. D. Galanin, B. P. Kirsanov, and Z. A. Chizhikova, Pis’ma Zh. Eksp. Teor. Fiz. 11 (1), 157 (1970).
H.-B. Lin and M. R. Topp, Chem. Phys. Lett. 47 (3), 442 (1977).
V. L. Ermolaev and V. A. Lyubimtsev, Optika Spektrosk. 60 (1), 74 (1986).
H. Fukumura, K. Kikuchi, K. Koike, and H. Kokubun, J. Photochem. Photobiol. 42 (2–3), 283 (1988).
S. N. Letuta, U. G. Letuta, and S. N. Pashkevich, Biophysics (Moscow) 64 (4), 576 (2019).
V. V. Ryl’kov and E. A. Cheshev, Dokl. Akad. Nauk SSSR 281 (3), 648 (1985).
V. V. Ryl’kov and E. A. Cheshev, Optika Spektrosk. 63 (5), 1030 (1987).
S. N. Letuta, Vestn. OGU 5, 88 (2002).
K. K. Rohatgi-Mukherjee and A. K. Mukhopadhyay, Indian J. Pure Appl. Phys. 14 (6), 481 (1976).
L. V. Levshin and A. M. Saletskii, Luminescence and Its Measurement (Moscow State Univ., Moscow, 1989) [in Russian].
N. Kashef, Y.-Y. Huang, M. R. Hamblin, Nanophotonics 6 (5), 853 (2017).
A. A. Aleksandrov, V. A. Belogol’skii, V. I. Levtsov, et al., SSSRD 190-2000: Tables of Standard Reference Data. Water: Speed of Sound at Temperatures of 0 to 100 Degrees Centigrade and Pressures of 0.101325 to 100 MPa (Izd. Standartov, Moscow, 2000) [in Russian].
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The work was supported by the Ministry of Education and Science of the Russian Federation, project FSGU-2020-0003.
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Translated by E. V. Makeeva
Abbreviations: HEES, highly excited electronic states.
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Letuta, S.N., Pashkevich, S.N., Ishemgulov, A.T. et al. Photothermal Inactivation of Microorganisms under Relaxation of Highly Excited States of Sensitizers. BIOPHYSICS 65, 599–605 (2020). https://doi.org/10.1134/S0006350920040089
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DOI: https://doi.org/10.1134/S0006350920040089