Abstract
An experiment was conducted in a split plot factorial design with 3 replications for evaluating the physiological responses of cumin (Cuminum cyminum L.) to different times and types of stress modulators under rain-fed and irrigated conditions. The main factors included cultivation method at the 2 levels of rain-fed and irrigated conditions and foliar application times (once in the vegetative phase and twice in the vegetative and flowering stages) and sources of stress modulators at the 4 levels of control, 1.5 M glycine betaine (GB), 10–7 mM brassinosteroids (BRs), and 1.5 μM sodium nitroprusside (SNP). The rain-fed cultivations included more carotenoids, H2O2, proline, GB content, catalase, superoxide dismutase, and guaiacol peroxidase activities and less chlorophyll a, while further chlorophyll b appeared in the irrigated cultivations. The application times had no significant effects on the plant’s physiological attributes. Both in the rain-fed and irrigated conditions, foliar applications of BRs increased the activities of enzymatic or non-enzymatic antioxidants in cumin as compared to the other osmotic modulators. Under the rain-fed conditions, spraying of BRs increased the contents of chlorophyll a, chlorophyll b, carotenoids, and proline and catalase, superoxide dismutase, and guaiacol peroxidase activities compared to the control, while decreasing H2O2 and glycine betaine contents. Under the irrigated conditions, the amounts of chlorophyll a, chlorophyll b, carotenoids, proline, and GB and catalase, superoxide dismutase, and guaiacol peroxidase activities enhanced, whereas H2O2 content were lowered in comparison to the control. Overall, the applied modulators could be classified as brasinosteroids > glycine betaine > sodium nitroprusside in terms of reducing drought stress impacts through their modulatory effects of enhancing enzymatic antioxidant activities.
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REFERENCES
Ashraf, M. and Foolad, M., Improving plant abiotic-stress resistance by exogenous application of osmoprotectants glycine betaine and proline, Environ. Exp. Bot., 2007, vol. 59, p. 206.
Salehi-Lisar, S.Y. and Bakhshayeshan-Agdam, H., Drought stress in plants: causes, consequences, and tolerance, Drought Stress Tolerance in Plants, Springer, 2016, vol. 1, p.1.
Fariduddin, Q., Khanam, S., Hasan, S., Ali, B., Hayat, S., and Ahmad, A., Effect of 28-homobrassinolide on the drought stress-induced changes in photosynthesis and antioxidant system of Brassica juncea L., Acta Physiol. Plant., 2009, vol. 31, p. 889.
Armin, M. and Miri, H.R., Effects of glycine betaine application on quantitative and qualitative yield of cumin under irrigated and rain-fed cultivation, J. Essent. Oil Bear. Pl., 2014, vol. 17, p. 708.
Miri, H.R. and Zamani, M.A., The effect of external usage of glycine betaine on corn (Zea mays L.) in drought condition, Iranian J. Field Crops Res., 2015, vol. 12, p. 704.
Roychoudhury, A. and Banerjee, A., Endogenous glycine betaine accumulation mediates abiotic stress tolerance in plants, Trop. Plant Res., 2016, vol. 3, p. 105.
Yuan, L., Shu, S., Sun, J., Guo, S., and Tezuka, T., Effects of 24-epibrassinolide on the photosynthetic characteristics, antioxidant system, and chloroplast ultrastructure in Cucumis sativus L. under Ca(NO3)2 stress, Photosynth. Res., 2012, vol. 112, p. 205.
Talaat, N.B., Shawky, B.T., and Ibrahim, A.S., Alleviation of drought-induced oxidative stress in maize (Zea mays L.) plants by dual application of 24-epibrassinolide and spermine, Environ. Exp. Bot., 2015, vol. 113, p. 47.
Li, K.R. and Feng, C., Effects of brassinolide on drought resistance of Xanthoceras sorbifolia seedlings under water stress, Acta Physiol. Plant., 2011, vol. 33, p. 1293.
Lei, Y., Yin, C., Ren, J., and Li, C., Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings, Biol. Plant., 2007, vol. 51, p. 386.
Farooq, M., Nawaz, A., Chaudhary, M., and Rehman, A., Foliage-applied sodium nitroprusside and hydrogen peroxide improves resistance against terminal drought in bread wheat, J. Agron. Crop Sci., 2017, vol. 203, p. 473.
Neill, S., Barros, R., Bright, J., Desikan, R., Hancock, J., Harrison, J., Morris, P., Ribeiro, D., and Wilson, I., Nitric oxide, stomatal closure, and abiotic stress, J. Exp. Bot., 2008, vol. 59, p. 165.
Arnon, A., Method of extraction of chlorophyll in the plants, Agron. J., 1967, vol. 23, p. 112.
Bates, L.S., Waldren, R.P., and Teare, I., Rapid determination of free proline for water-stress studies, Plant Soil, 1973, vol. 39, p. 205.
Grieve, C. and Grattan, S., Rapid assay for determination of water soluble quaternary ammonium compounds, Plant Soil, 1983, vol. 70, p. 303.
Alexieva, V., Sergiev, I., Mapelli, S., and Karanov, E., The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat, Plant Cell Environ., 2001, vol. 24, p. 1337.
Aebi, H., Catalase in vitro, in Methods in Enzymology, Elsevier, 1984, p. 121.
Beauchamp, C. and Fridovich, I., Superoxide dismutase: improved assays and an assay applicable to acrylamide gels, Anal. Biochem., 1971, vol. 44, p. 276.
Fielding, J. and Hall, J., A biolchemical and cytochemical study of peroxidase activity in roots of Pisum sativum. I. A comparison of DAB-peroxidase and guaiacol-peroxidase with particular emphasis on the properties of cell wall activity, J. Exp. Bot., 1978, vol. 29, p. 969.
Oukarroum, A., Schansker, G., and Strasser, R.J., Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance, Physiol. Plant., 2009, vol. 137, p. 188.
Blokhina, O., Virolainen, E., and Fagerstedt, K.V., Antioxidants, oxidative damage and oxygen deprivation stress: a review, Ann. Bot., 2003, vol. 91, p. 179.
Ahmed, N., Zhang, Y., Li, K., Zhou, Y., Zhang, M., and Li, Z., Exogenous application of glycine betaine improved water use efficiency in winter wheat (Triticum aestivum L.) via modulating photosynthetic efficiency and antioxidative capacity under conventional and limited irrigation conditions, Crop J., 2019, vol. 7, p. 635.
Bajguz, A. and Hayat, S., Effects of brassinosteroids on the plant responses to environmental stresses, Plant Physiol. Bioch., 2009, vol. 47, p. 1.
Reise, M., Asrar, Z., and Poursaeid, S., Interaction of sodium nitroprusside (SNP) and copper on growth parameters and physiology (Lepidium sativum L.), J. Plant Biol., 2010, vol. 1, p. 55.
Kočová, M., Rothová, O., Holá, D., Kvasnica, M., and Kohout, L., The effects of brassinosteroids on photosynthetic parameters in leaves of two field-grown maize inbred lines and their F1 hybrid, Biol. Plant., 2010, vol. 54, p. 785.
Ashraf, M. and Foolad, M., Roles of glycine betaine and proline in improving plant abiotic stress resistance, Environ. Exp. Bot., 2007, vol. 59, p. 206.
Sartip, H. and Sirousmehr, A.R., Evaluation of salicylic acid effects on growth, yield and some biochemical characteristics of cumin (Cuminum cyminum L.) under three irrigation regimes, Environmental Stresses in Crop Sciences, 2017, vol. 10, p. 547.
Anwar, A., Liu, Y., Dong, R., Bai, L., Yu, X., and Li, Y., The physiological and molecular mechanism of brassinosteroid in response to stress: a review, Biol. Res., 2018, vol. 51, p. 46.
Sahni, S., Prasad, B.D., Liu, Q., Grbic, V., Sharpe, A., Singh, S.P., and Krishna, P., Overexpression of the brassinosteroid biosynthetic gene DWF4 in Brassica napus simultaneously increases seed yield and stress tolerance, Sci. Rep., 2016, vol. 6, p. 28298.
Verbruggen, N. and Hermans, C., Proline accumulation in plants: a review, Amino Acids, 2008, vol. 35, p. 753.
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Timachi, F., Armin, M., Jamimoeini, M. et al. Physiological Response of Cumin to Times and Type of Stress Modulator in Rain-Fed and Irrigated Conditions. Russ J Plant Physiol 67, 1163–1172 (2020). https://doi.org/10.1134/S1021443720060175
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DOI: https://doi.org/10.1134/S1021443720060175