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CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato

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CRISPR/Cas9-based multiplexed editing of SlHyPRP1 resulted in precise deletions of its functional motif(s), thereby resulting in salt stress-tolerant events in cultivated tomato.

Abstract

Crop genetic improvement to address environmental stresses for sustainable food production has been in high demand, especially given the current situation of global climate changes and reduction of the global food production rate/population rate. Recently, the emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-based targeted mutagenesis has provided a revolutionary approach to crop improvement. The major application of CRISPR/Cas in plant genome editing has been the generation of indel mutations via error-prone nonhomologous end joining (NHEJ) repair of DNA DSBs. In this study, we examined the power of the CRISPR/Cas9-based novel approach in the precise manipulation of protein domains of tomato hybrid proline-rich protein 1 (HyPRP1), which is a negative regulator of salt stress responses. We revealed that the precise elimination of SlHyPRP1 negative-response domain(s) led to high salinity tolerance at the germination and vegetative stages in our experimental conditions. CRISPR/Cas9-based domain editing may be an efficient tool to engineer multidomain proteins of important food crops to cope with global climate changes for sustainable agriculture and future food security.

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Acknowledgments

We wish to thank Mrs. Jeong Se Jeong for her valuable technical support in this study. This work was supported by the National Research Foundation of Korea (Grant NRF 2020R1I1A1A01072130, 2020M3A9I4038352, 2020R1A6A1A03044344), the Next-Generation BioGreen 21 Program (SSAC, Grant PJ01322601) and the Program for New Plant Breeding Techniques (NBT, Grant PJ01478401), Rural Development Administration (RDA), Korea.

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Correspondence to Tien Van Vu or Jae-Yean Kim.

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The authors declare that they have no competing interests.

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Communicated by Günther Hahne.

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Tran, M.T., Doan, D.T.H., Kim, J. et al. CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato. Plant Cell Rep 40, 999–1011 (2021). https://doi.org/10.1007/s00299-020-02622-z

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  • DOI: https://doi.org/10.1007/s00299-020-02622-z

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