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Development and evaluation of improved lines based on an elite rice variety 9311 for overcoming hybrid sterility in rice

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Abstract

Hybrid sterility is a major obstacle to the development of superior inter-subspecific hybrids between indica and japonica subspecies of Asian-cultivated rice. To overcome hybrid sterility, we introduced four japonica alleles (S-j) at Sa, Sc, f5, and S32 loci from Nipponbare into 9311 through marker-assisted backcross breeding, and four improved lines were obtained. Three wide-compatibility lines 9311(S5-n), 9311(f5-n), and 9311(S5-n+f5-n) previously constructed were used as control and parental line of hybridization. By crossing with 9311(S5-n+f5-n), two pyramiding lines 9311(Sa+S5-n+f5-n) and 9311(S32+Sa+S5-n+f5-n) were obtained. To evaluate the gene effect of pollen fertility and spikelet fertility, the near-isogenic lines (NILs) harboring S5-n, f5-n, Sa, Sc, f5, or S32 and three pyramiding lines with S5-n+f5-n, Sa+S5-n+f5-n, or S32+Sa+S5-n+f5-n were testcrossed to six japonica varieties. The results showed that single-gene f5, f5-n, S32, and S5-n could significantly improve spikelet fertility in majority of indica-japonica hybrids. Single-gene Sa and Sc could significantly improve spikelet fertility in part of indica-japonica hybrids. Moreover, the pyramiding lines exhibited significantly higher spikelet fertility than the control in indica-japonica hybrids. A significant increase in spikelet fertility (31.4–55%) was observed in three- or four-gene pyramiding lines. Our results provide an effective approach to exploiting heterosis between indica and japonica subspecies, which had a profound implication in rice breeding.

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Acknowledgments

We are very grateful to Professor Tongmin Mou (Huazhong Agricultural University) for providing seeds of the wide-compatibility lines. Great gratitude goes to linguistics professor Ping Liu from Foreign Language College, Huazhong Agriculture University, Wuhan, China, for her work at English editing and language polishing.

Funding

This work was supported by grants from the National Natural Science Foundation of China (31801438).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

    Xiaojuan Ma, Xin Wei, Guanjun Gao & Haichao Jiang

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  1. Xiaojuan Ma
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  2. Xin Wei
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  3. Guanjun Gao
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  4. Haichao Jiang
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HJ conceived and designed the experiments; XM, XW, and GG performed the experiments; HJ and XM analyzed the data; HJ and XM wrote the manuscript.

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Correspondence to Haichao Jiang.

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Ma, X., Wei, X., Gao, G. et al. Development and evaluation of improved lines based on an elite rice variety 9311 for overcoming hybrid sterility in rice. Mol Breeding 40, 102 (2020). https://doi.org/10.1007/s11032-020-01180-2

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