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CbCYP716A261, a New β-Amyrin 28-Hydroxylase Involved in Conyzasaponin Biosynthesis from Conyza blinii

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Abstract

Conyzasaponins produced by the traditional Chinese herb Conyza blinii are oleanane-type saponins with a wide range of biological activities. Here, we identified a gene, designated CbCYP716A261, encoding a β-amyrin 28-hydroxylase in conyzasaponins biosynthesis. Ten full putative CYP sequences were isolated from Conyza blinii transcript tags. The CbCYP716A261 gene product was selected as the putative β-amyrin 28‑hydroxylase by phylogenetic analysis and transcriptional activity analysis of methyl jasmonate-treated Conyza blinii. To identify the enzymatic activity, we performed enzymatic activity experiments in vitro and in vivo. The HPLC results revealed that CbCYP716A261 catalyzes the hydroxylation of β-amyrin at the C-28 position to yield oleanolic acid. Our findings provide new information about the conyzasaponin biosynthesis pathway and widen the list of isolated β-amyrin 28-hydroxylases.

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Authors and Affiliations

  1. College of Life Science, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China

    R. Sun, H. Chen & Z. Z. Tang

  2. College of Biological and Chemical Engineering, Panzhihua University, 617000, Panzhihua, Sichuan, China

    R. Sun, J. L. Gao & S. Liu

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Correspondence to H. Chen.

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FUNDING

This study was supported by a grant of the Panzhihua University (035200167) and Department of Science and Technology of Sichuan Province (2018HH0074).

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This article does not contain any studies involving animals or human participants performed by any of the authors.

The authors declare that they have no conflict of interest.

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The text was submitted by the author(s) in English.

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Abbreviations: HPLC, High-performance liquid chromatography; CYP, Cytochrome P450; GT, Glycosyltransferase; MeJA, Methyl jasmonate; SC-U, Synthetic complete medium lacking uracil.

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Sun, R., Gao, J.L., Chen, H. et al. CbCYP716A261, a New β-Amyrin 28-Hydroxylase Involved in Conyzasaponin Biosynthesis from Conyza blinii . Mol Biol 54, 719–729 (2020). https://doi.org/10.1134/S002689332005009X

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