Abstract
A novel molecularly imprinted ratiometric fluorescent probe was fabricated by simple sol–gel polymerization for selective and sensitive assay of C-type natriuretic peptide (CNP) in biosamples. Both the nitrobenzoxadiazole (NBD) and carbon dots (CDs) were located on the surface of silica, used as the detection signal and reference signal, respectively. For the turn-on-based probe, the fluorescence intensity of NBD could be quantitatively enhanced by CNP based on the strategy of photo-induced electron transfer (PET), while the fluorescence of CDs remained unchanged. The obtained probe exhibited excellent recognition selectivity and fast kinetics to CNP templates, and also showed good stability. The linear range of CNP determination was 5–80 pg mL−1 with a low detection limit of 2.87 pg mL−1. Finally, the probe was successfully applied to determine CNP in human serum samples and attained high recoveries between 97.3 and 104% with precisions below 4.7%. The result indicates that the proposed method has promising potential for the assay of trace peptides in complex matrices.
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Funding
This work was financially supported by the Natural Science Foundation of Jiangsu Province (BK20191352), Jiangsu Salt Industry Group Co., Ltd. (NMU-SY201805), and Nanjing Health Science and Technology Development Special Fund (YKK19173) in China.
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All work presented was reviewed and approved by the Ethics Committee of Sir Run Run Hospital, Nanjing Medical University. The serum samples were donated under informed consent.
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Hongliang He and Min Cao are co-first authors
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He, H., Cao, M., Hu, J. et al. Fluorescent turn-on assay of C-type natriuretic peptide using a molecularly imprinted ratiometric fluorescent probe with high selectivity and sensitivity. Microchim Acta 187, 614 (2020). https://doi.org/10.1007/s00604-020-04583-2
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DOI: https://doi.org/10.1007/s00604-020-04583-2