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Cytotoxic Activity Assessment and GC-MS Screening of Two Codium Species Extracts

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Pharmaceutical Chemistry Journal Aims and scope

Various solvent extracts from two marine green algae, Codium bursa and Codium fragile collected from the North Tunisian coasts during spring and summer seasons were used to obtain high dry matter yields. The algal extracts showed the great power of in vitro cell growth inhibition against Hela cells, which was confirmed by MTT test. The comparative efficiency of different polarity solvents showed that methanolic extract with high-polarity composition had significantly more important yield than petrol ether extracts, while the higher inhibition activity was obtained with petrol ether extract from C. bursa (IC50= 115.755 μg/mL). Moreover, it showed a seasonal variation that revealed the most important activity during the season of spring as compared to summer. Flash chromatography of the petrol ether extract and GC-MS analysis of its most active fraction revealed a total of six major compounds well-known in medicinal plants: 6,10,14-trimethyl-2-pentadecanone; 2-dodecanone; 1-dodecanol; dodecyl acrylate; formic acid 3,7,11-trimethyl-1,6,10-dodecatrien-3-yl ester; and bicyclo[4.4.0]dec-2-ene-4-ol 2-methyl-9-(prop-1-en-3-ol-2-yl).

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References

  1. K. O’Sullivan, P. O’Dea,W. Dankaerts, et al., Man. Ther., 15(6), 557–561 (2010).

    Google Scholar 

  2. A. Jimenez-Escrig, E. Gomez-Ordonez, and P. Ruperez, J. Appl. Phycol., 24(5), 1123 – 1132 (2012).

    CAS  Google Scholar 

  3. S. Celikler, O. Vatan, G. Yildiz, and R. Bilaloglu, Food. Chem. Toxicol., 47(4), 796 – 801 (2009).

    CAS  Google Scholar 

  4. S. Caccamese and R. Azzolina, Planta. Med., 37(12), 333 – 339 (1979).

    CAS  Google Scholar 

  5. H. Harada and Y. Kamei, Cytotechnology, No. 25, 213 – 219 (1997).

    CAS  Google Scholar 

  6. A. K. Siddhanta, K. H. Mody, B. K. Ramavat, el al., Indian. J. Exp. Biol., 35(6), 638 – 643 (1997).

    CAS  Google Scholar 

  7. L. Misurcova, S. Kraemar, B. Klejdus, and J. Vacek, Czech. J. Food. Sci., 28(1), 27 – 35 (2010).

    CAS  Google Scholar 

  8. A. J. De Andrade, P. H. Shimabukuro, and E. A. Galati, Zootaxa, No. 3734, 477 – 484 (2013).

  9. M. H. Norziah and Y. Ch. Ching, Food. Chem., No. 68, 69 – 76 (2002).

  10. L. Misurcova, S. Kraèmar, B. Klejdus, and J. Vacek, Czech. J. Food Sci., 28(1), 27 – 35 (2010).

    CAS  Google Scholar 

  11. J. Gauber, C. E. Payri, C. Vieira, et al., Sci. Rep., 9(1), 993 (2019).

    Google Scholar 

  12. B. Martinez and J. M. Rico, J. Phycol., 38 (6), 1082–1089 (2002).

    CAS  Google Scholar 

  13. E. Marinho Soriano, P. C. Fonseca, M. A. A. Carneiro, and W. S. C. Moreira, Biores. Technol., 97(18), 2402 – 2406 (2006).

    CAS  Google Scholar 

  14. S. Marsham, G. W. Scott, and M. L. Tobin, Food. Chem., 100(4), 1331 – 1336 (2007).

    CAS  Google Scholar 

  15. S. Gupta and N. Abu-Ghannam, Trends Food. Sci. Technol., 22(6), 315 – 326 (2011).

    CAS  Google Scholar 

  16. Y. L. Lee, G. W. Huang, Z. C. Liang, and J. L. Mau, Lebensm. Wiss. Technol., 40, 823 – 833 (2007).

    CAS  Google Scholar 

  17. A. Barchan, M. Bakkali, A. Arakrak, et al., Int. J. Curr. Microbiol. Appl. Sci., 3(11), 399 – 412 (2014).

    Google Scholar 

  18. A. Hoffmann and B. Santelices, Flora Marina De Chile Central, Ediciones Universidad Catolica de Chile: Santiago, Chile (1997).

    Google Scholar 

  19. NIST Mass Spectral Library, Search 2.2.

  20. T. Mosman, J. Immunol. Meth., No. 65, 55 – 63 (1983).

  21. J. Heilmann, M. R. Wasescha, and T. J. Schmidt, Bioorg, Med. Chem., 9(8), 2189–2194 (2001).

    CAS  Google Scholar 

  22. F. Frikha, M. Kammoun, N. Hammami, et al. Cienc. Mar., 37(2), 113 – 124 (2011).

    CAS  Google Scholar 

  23. K. S. Kumar, K. Ganesan, and P. V. S. Rao, Food Chem., 107(1), 289 – 295 (2008).

    CAS  Google Scholar 

  24. L. C. Irkin and H. Erdugan, Turkey. Mar. Sci. Tech. Bull., 3(1), 1 – 4 (2014).

    Google Scholar 

  25. G. Jiao, G. Yu, J. Zhang, and H. Ewart, Mar. Drugs., 9(2), 196 – 223 (2011).

    CAS  Google Scholar 

  26. S. Patel, 3Biotech., 2(3), 171 – 185 (2012).

    Google Scholar 

  27. S. Rajeshkumar, C. Malarkodi, G. Gnanajobitha, et al., J. Nanostructure. Chem., 3(1), 44 (2013).

    Google Scholar 

  28. R. Aliya, M. Shameel, K. Usmanghani, and V. U. Ahmad, Pak. J. Pharm. Sci., 4(2), 103 – 11 (1991).

    CAS  Google Scholar 

  29. S. V. Khotimchenko, Bot. Mar., 46(5), 456 – 460 (2003).

    CAS  Google Scholar 

  30. F. Goecke, V. Hernandez, M. Bittner, et al., Rev. Biol. Mar. Oceanogr., 45(2), 325 – 330 (2010).

    Google Scholar 

  31. V. U. Ahmad, R. Aliya, S. Perveen, and M. Shameel, Phytochemistry., 33(5), 1189 – 1192 (1993).

    CAS  Google Scholar 

  32. R. Kapetanoviæ, D. Sladiæ, S. Popov, et al., J. Serb. Chem. Soc., 70(12), 1395 – 1400 (2005).

    Google Scholar 

  33. G. Lopes, C. Sousa, P. Valentao and P. B. Andrade, Bioactive Compounds from Marine Foods, B. Hernandez-Ledesma and M. Herrero (Eds.), Wiley: Chichester (2013), pp. 173 – 191.

  34. E. E. Benson, A. H. Cobb, New. Phytol., 95(4), 581 – 594 (1983).

    CAS  Google Scholar 

  35. M. Tabarsa, S. Karnjanapratum, M. Cho, et al., Int. J. Biol. Macromol., No. 59, 1 – 12 (2013).

  36. U. N. Ekwenye and N. N. Elegalam, Int. J. Mol. Med., No. 1, 411 – 417 (2005).

  37. L. Zhu, L. Zhang, X. Lu, et al., J. Ethnopharmacol., 151(1), 576 – 582 (2014).

    CAS  Google Scholar 

  38. M. J. Abad, M. Ansuategui, P. Bermejo, Arkivoc: Arch. Org. Chem., No. 7, 116 – 145 (2006).

    Google Scholar 

  39. L. H. Lee, K. G. Chan, J. Stach, et al., Front. Microbiol., No. 9, 824 (2018).

  40. A. Manilal, S. Sujith, J. Selvin, et al., J. Mar. Sci. Tech-Japan., 17(1), 67 – 73 (2009).

    Google Scholar 

  41. D. Hajjar, S. Kremb, S. Sioud, et al., Plos One, 12(6), e0177316 (2017).

    Google Scholar 

  42. M. Esghaei, H. Ghaffari, B. R. Esboei, et al., Asian. Pac. J. Cancer. Prev., 19(6), 1697 – 1701 (2018).

  43. N. Togashi, A. Shiraishi, M. Nishizaka, et al., Molecules, 12(2), 139 – 148. 2007.

    CAS  Google Scholar 

  44. P. Kathirvel, K. Poonkodi, K. Gomathi, and M. Akila, Asian. J. Pharm. Clin. Res., 10(5), 297 (2017).

    Google Scholar 

  45. S. Guleria, A. K. Tiku, A. Koul, et al., Sci. World. J., 2013(2), 790580 (2013).

    Google Scholar 

  46. D. H. Wang and W. Y. Tao, Afr. J. Microbiol. Res., 3(11), 637 – 640 (2009).

    Google Scholar 

  47. S. Arora and G. Kumar, Int. Res. J. Pharm., 8(6), 2230 – 8407 (2017).

    Google Scholar 

  48. R. Valls, J. Artaud, P. Amade, et al., J. Chromatogr. A, No. 663, 114 – 118 (1994).

  49. R. Mozzachiodi, R. Scuri, M. Roberto, and M. Brunelli, Neuroscience., 107(3), 519 – 526 (2001).

    CAS  Google Scholar 

  50. D. J. W. Blum and R. E. Speece, Crit. Rev., 24(3), 284 – 293 (1990).

    CAS  Google Scholar 

  51. A. Manilal, S. Sujith, J. Selvin, et al., J. Mar. Sci. Tech-Japan., 17(1), 67 – 73 (2009).

    Google Scholar 

  52. A. Manilal, S. Sujith, J. Selvin, et al., Sci. Mar., 74(2), 287 – 296 (2010).

    Google Scholar 

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Acknowledgements

We would like to express our thanks to Prof. Dr. Jorg Heilmann, the head of Department of Pharmaceutical Biology, University of Regensburg, Germany, and Dr. Guido Juergenliemk, senior scientific advisor, University of Regensburg, Germany, for their great help and support.

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

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  1. Department of Biology, University of Tunis-El-Manar, 2092, Tunis, Tunisia

    Nidhal Fahem, Aslam Sami Djellouli & Sellema Bahri

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  1. Nidhal Fahem
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  2. Aslam Sami Djellouli
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  3. Sellema Bahri
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Correspondence to Sellema Bahri.

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Fahem, N., Djellouli, A.S. & Bahri, S. Cytotoxic Activity Assessment and GC-MS Screening of Two Codium Species Extracts. Pharm Chem J 54, 755–760 (2020). https://doi.org/10.1007/s11094-020-02266-z

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