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Impact of pesticides in properties of Bradyrhizobium spp. and in the symbiotic performance with soybean

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Abstract

Soybean [Glycine max (L.) Merr.] has great economic and nutritional importance mainly due to its high protein content. All plant's N needs can be met by the symbiosis with elite Bradyrhizobium strains applied as inoculants to the seeds at sowing time; however, the increasing use of pesticides in seed treatments can impair the contribution of the biological nitrogen fixation. In this study, we report decreases in cell survival of two strains, B. japonicum SEMIA 5079 and B. elkanii SEMIA 587 in seeds inoculated and treated with StandakTop™, composed of the fungicides pyraclostrobin and thiophanate-methyl and the insecticide fipronil, the pesticides most used in soybean seed treatment in several countries. Cell death was enhanced with the time of exposure to the pesticides, and B. elkanii was less tolerant, with almost no detectable viable cells after 15 days. Change in colony morphology with smaller colonies was observed in the presence of the pesticides, being more drastic with the time of exposure, and attributed to an adaptive response towards survival in the presence of the abiotic stress. However, morphological changes were reversible after elimination of the stressing agent and symbiotic performance under controlled greenhouse conditions was similar between strains that had been or not exposed to the pesticides. In addition, no changes in DNA profiles (BOX-PCR) of both strains were observed after the contact with the pesticides. In two field experiments, impacting effects of the pesticides were observed mainly on the total N accumulated in grains of plants relying on both N2-fixation and N-fertilizer. Our data indicate that StandakTop® affects parameters never reported before, including colony morphology of Bradyrhizobium spp. and N metabolism and/or N remobilization to soybean grains.

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Data availability

All datasets generated or analyzed during this study are included in the manuscript, and complementary dataset will be available upon request to the corresponding author.

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Acknowledgements

T. F. Rodrigues acknowledges an MSc fellowship and F.R. Bender and A.W.S. Sanzovo PhD fellowships from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil—Finance Code 001). Author acknowledge to Dr. Artur B. L. Rondina for suggestions on the study and on the manuscript. M.A. Nogueira and M. Hungria are also research fellows from CNPq (Brazilian National Research Council for Science and Technology).

Funding

Funded by INCT-Plant-Growth Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility (CNPq 465133/2014–2, Fundação Araucária-STI-043/2019, CAPES), Embrapa, CNPq-Universal (400468/2016–6).

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, C.P. 10.011, Londrina, Paraná, 86.057-970, Brazil

    Thiago Fernandes Rodrigues, Flavia Raquel Bender & Mariangela Hungria

  2. Embrapa Soja, Caixa Postal 231, Londrina, Paraná, CEP 86001-970, Brazil

    Thiago Fernandes Rodrigues, Flavia Raquel Bender, Alisson Wilson Santos Sanzovo, Eduara Ferreira, Marco Antonio Nogueira & Mariangela Hungria

  3. Department of Agronomy, Universidade Estadual de Londrina, C.P. 6001, Londrina, Paraná, 86.057-970, Brazil

    Alisson Wilson Santos Sanzovo & Marco Antonio Nogueira

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  1. Thiago Fernandes Rodrigues
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  2. Flavia Raquel Bender
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  4. Eduara Ferreira
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Contributions

Conceived and designed the experiments: TFR, MAN, MH. Performed the experiments: TFR, FRB, AWSS, EF. Analyzed the data: TFR, FRB, AWSS, EF, MAN, MH. Contributed reagents/materials/analysis tools: MH Wrote the paper: TRF, MAN, MH. All authors read and approved the final manuscript.

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Correspondence to Mariangela Hungria.

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Rodrigues, T.F., Bender, F.R., Sanzovo, A.W.S. et al. Impact of pesticides in properties of Bradyrhizobium spp. and in the symbiotic performance with soybean. World J Microbiol Biotechnol 36, 172 (2020). https://doi.org/10.1007/s11274-020-02949-5

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