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Relative toxicity and insecticide resistance of different field population of tomato leaf miner, Tuta absoluta (Meyrick)

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Abstract

South American tomato moth, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is an oligophagous insect pest that infests solanaceous crops like tomato, brinjal and potato. The incidence of T. absoluta could cause significant yield losses of up to 100% on tomato crop and therefore farmers are following chemical management practices to tackle the pest. However, continuous and indiscriminate usage of certain insecticides may lead to development of resistance in T. absoluta. The aim of the study therefore was to know the level of resistance to different commonly used insecticides viz., indoxacarb14.5SC, flubendiamide 39.35SC, emamectin benzoate 5SG, spinosad 45SC, spinetoram 11.1SC and cyantraniliprole 10.25SC from different field population of T. absoluta viz., Bangalore and Kolar districts of Karnataka, Madurai and Salem districts of Tamil Nadu and Ananthpur district in Andhra Pradesh, India. A variable level of susceptibility of different field population of T. absoluta was noticed against frequently used insecticides viz. flubendiamide 39.35SC, cyantraniliprole 10.25SC and indoxacarb14.5SC. Biochemical assays indicated a significant increase in cytochrome P450 monooxygenase and esterase level in all relatively resistant population of T. absoluta. The cytochrome P450 monooxygenase and esterase enzymes therefore play a major role in the development of resistance in field population of T. absoluta. The reduced susceptibility of T. absoluta to various field collected population thus indicates a development of resistance to various insecticides necessitating the judicious use of chemicals for its management.

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References

  • Andrew GS, Cuthbertson JJM, Lisa FB, Anastasia K, Weiqi L, Robert JJ, Phil N (2013) Population development of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under simulated UK glasshouse conditions. Insects 4(2):185–197

    Google Scholar 

  • Astor E, Scals D (2009) The control of Tuta absoluta with insecticides compatible with integrated pest management programmers and the prevention of resistance. Agricola Vergel: Fruticult Horticult Floricult Citricult Vid, Arroz 28:492–495

    Google Scholar 

  • Berge BJ, Feyereisen R, Amichot M (1998) Cytochrome P450 monooxygenases and insecticide resistance in insects. Royal Society Lond 353:1701–1705

    CAS  Google Scholar 

  • Brogdon WG (1989) Biochemical resistance detection: an alternative to bioassay. Parasitol Today 5:56–60

    CAS  PubMed  Google Scholar 

  • Desneux NE, Wajnberg KG, Wyckhuys G, Burgio Arpaia C, Narvaez-Vasquez J, González-Cabrera D, Catalán RE, Tabone J, Frandon J, Pizzol C, Poncet TC, Urbaneja A (2010) Biological invasion of European tomato crops by Tuta absoluta: ecology, geographic expansion and prospects for biological control. J Pestic Sci 83:197–215

    Google Scholar 

  • Emmanouil R, Konstantinos M, Maria R, Emmanouil V, Evangelia M, Jean LR, Vontas J (2017) Identification and detection of indoxacarb resistance mutations in the Para sodium channel of the tomato leaf miner, Tuta absoluta. Pest Manag Sci 73:1679–1688

    Google Scholar 

  • Enayati AA, Lengeler C, Erlanger T, Hemingwaya J (2005) Field evaluation of a recombinant glutathione S-transferase-based pyrethroid quantification assay. Trans R Soc Trop Med Hyg 99:369–378

    CAS  PubMed  Google Scholar 

  • Grant C, Jacobson R, Ilias A et al (2019) The evolution of multiple-insecticide resistance in UK populations of tomato leafminer, Tuta absoluta. Pest Manag Sci 75:2079–2085

    CAS  PubMed  Google Scholar 

  • Guedes RNC, Roditakis E, Campos MR, Haddi K, Bielza P, Siqueira HAA, Tsagkarakou A, Vontas J, Nauen R (2019) Insecticide resistance in the tomato pinworm Tuta absoluta: patterns, spread, mechanisms, management and outlook. J Pest Sci 92:1329–1342

    Google Scholar 

  • Habig WH, Pabst MJ, Fleischner G, Gatmaitan Z, Arias IM, Jakoby WB (1974) The identity of glutathione S-transferase B with ligandin, a major binding protein of liver. Proc Natl Acad Sci U S A 71:3879–3882

    CAS  PubMed  PubMed Central  Google Scholar 

  • Heidari R, Devonshire AL, Campbell BE, Dorrian SJ, Oakeshott JG (2005) Hydrolysis of pyrethroids by carboxylesterases from Lucilia cuprina and Drosophila melanogaster with active sites modified by in vitro mutagenesis. Insect Biochem Mol Biol 35(6):597–609

    CAS  PubMed  Google Scholar 

  • Huang H, Ottea JA (2004) Development of pyrethroid substrates for esterases associated with pyrethroid resistance in the tobacco budworm, Heliothis virescens (F.). J Agric Food Chem 52(21):6539–6545

    CAS  PubMed  Google Scholar 

  • Itokawa K, Komagata O, Kasai S, Okamura Y, Masada M, Tomita T (2010) Genomic structures of Cyp9m10 in pyrethroid resistant and susceptible strains of Culex quinquefasciatus. Insect Biochem Mol Biol 40:631–640

    CAS  PubMed  Google Scholar 

  • Jallow MA, Abdelhafiz A, Dahab MS, Albaho VY, Devi DGA, Binson MT (2019) Baseline susceptibility and assessment of resistance risk to flubendiamide and chlorantraniliprole in Tuta absoluta (Lepidoptera: Gelechiidae) populations from Kuwait. Appl Entomol Zool 54:91–99

    CAS  Google Scholar 

  • Joußen N, Agnolet S, Lorenz S, Schöne SE, Ellinger R, Schneider B et al (2012) Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. Proc Natl Acad Sci U S A 109:15206–15211

    PubMed  PubMed Central  Google Scholar 

  • Kasai S, Weereshinghe IS, Shono T, Tamakawa M (2000) Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus say. Insect Biochem Mol Biol 30(2):163–117

    CAS  PubMed  Google Scholar 

  • Kerns DL, Gaylor MJ (1992) Insecticide resistance in field populations of the cotton aphid (Homoptera: aphididae). J Econ Entomol 85:1–8

    CAS  Google Scholar 

  • Konus M (2014) Analyzing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide. Turkish J Biochem 39(3):291–297

    Google Scholar 

  • Kranthi KR (2005) Insecticide resistance –monitoring, mechanisms and management manual. Published by central institute for cotton research, Nagpur

    Google Scholar 

  • Lietti MMM, Botto E, Alzogaray RA (2005) Insecticide resistance inargentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotrop Entomol 34:113–119

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Marcela ML, Eduardo B, Raul AA (2005) Insecticide resistance in Ragentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotrop Entomol 34(1):113–119

    Google Scholar 

  • Margaritopoulos JT, Skouras P, Nikolaidou P, Manolikaki I (2007) Insecticide resistance status of Myzus persicae (Hemiptera: Aphididae), populations from peach and tobacco in mainland Greece. Pest Manag Sci 63(8):821–829

    CAS  PubMed  Google Scholar 

  • Melis Y, Serhan M, Leyla D, Kozaci CT (2015) Insecticide resistance in two populations of Tuta absoluta (Meyrick,1917) (Lepidoptera: Gelechiidae) from Turkey. Turk Entomol Derg 39(2):137–145

    Google Scholar 

  • Nauen R (2006) Insecticide mode of action: return of the ryanodine receptor. Pest Manag Sci 62:690–692

    CAS  PubMed  Google Scholar 

  • Pandey P, Vadivelmurugan I, Muthukumar V, Bagavathiannan SKM (2017) Impact of combined abiotic and biotic stresses on plant growth and avenues for crop improvement by exploiting physio-morphological traits. Front Plant Sci 8:537–555

    PubMed  PubMed Central  Google Scholar 

  • Prasannakumar NR, Jyothi N, Kumar GR, Saroja S, Sridhar V (2020) Studies on outbreak of tomato pinworm, Tuta absoluta (Meyrick) in South India and its differential susceptibility to insecticides. Pest Manag Hort Ecosys 26(1):97–103

    Google Scholar 

  • Radwan EMM, Taha H (2012) Toxic and biochemical effects of different insecticides on the tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Egypt Acad J of Biol Sci 4(1):1–10

    Google Scholar 

  • Reyes M, Rocha K, Alarcón L, Siegwart M, Sauphanor B (2012) Metabolic mechanisms involved in the resistance of field populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to Spinosad. Pestic Biochem Physiol 102(1):45–50

    CAS  Google Scholar 

  • Riveron JM, Irving H, Ndula M, Barnes KG, Ibrahim SS, Paine MJ et al (2013) Directionally selected cytochrome P450 alleles are driving the spread of pyrethroid resistance in the major malaria vector Anopheles funestus. Proc Natl Acad Sci U S A 110:252–257

    CAS  PubMed  Google Scholar 

  • Roditakis E, Skarmoutso C, Staurakaki M, Marıadel RM, Vidal GL, Bielza P, Haddi K, Rapisarda C, Rison JL, Bassie A, Teixeiraf L (2013a) Determination of baseline susceptibility of European populations of Tuta absoluta (Meyrick) to indoxacarb and chlorantraniliprole using a novel dip bioassay method. Pest Manag Sci 69:217–227

    CAS  PubMed  Google Scholar 

  • Roditakis EC, Skarmoutsou M, Staurakaki (2013b) Toxicity of insecticides to populations of tomato borer Tuta absoluta (Meyrick) from Greece. Pest Manag Sci 69:834–840

    CAS  PubMed  Google Scholar 

  • Salazar ER, Araya JE (1997) Deteccin de resistencia a insecticides en la polilla del tomate. Simiente 67:8–22

    Google Scholar 

  • Scott JG, Wen Z (2001) Cytochromes P450 of insects: the tip of the iceberg. Pest Manag Sci 57(10):958–967

    CAS  PubMed  Google Scholar 

  • Silva TBM, Silva WM, Campos MR et al (2016) Susceptibility levels of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to minor classes of insecticides in Brazil. Crop Prot 79:80–86

    CAS  Google Scholar 

  • Silva EJ, Liliana Mariada S, Ribeiro NV, Raul Narciso C, Guedes HAS (2019) Field evolved resistance to chlorantraniliprole in the tomato pinworm Tuta absoluta: inheritance, cross-resistance profile, and metabolism. J Pestic Sci 92:1421–1431

    Google Scholar 

  • Siqueira HAA, Guedes RNC, Picanco MC (2000) Insecticide resistance in populations of Tula absoluta (Lepidoptera: Gelechiidae). Agric For Entomol 2:147–153

    Google Scholar 

  • Sridhar V, Chakravarthy AK, Asokan R, Vinesh LS, Rebijith KB, Vennila S (2014) New record of the invasive south American tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in India. Pest Manag Hort Ecosys 20(2):148–154

    Google Scholar 

  • Stevenson BJ, Bibby J, Pignatelli P, Muangnoicharoen S, O’Neill PM, Lian LY et al (2011) Cytochrome P450 6M2 from the malaria vector Anopheles gambiae metabolizes pyrethroids: sequential metabolism of deltamethrin revealed. Insect Biochem Mol Biol 41:492–502

    CAS  PubMed  Google Scholar 

  • Vontas JG, Small GJ, Hemingway J (2001) Glutathione S-transferases as antioxidant defence agents confer pyrethroid resistance in Nilaparvata lugens. Biochem J 357:65–72

    CAS  PubMed  PubMed Central  Google Scholar 

  • Zhu F, Parthasarathy R, Bai H, Woithe K, Kaussmann M, Nauen R et al (2010) A brain-specific cytochrome P450 responsible for the majority of deltamethrin resistance in the QTC279 strain of Tribolium castaneum. Proc Natl Acad Sci U S A 107:8557–8562

    CAS  PubMed  PubMed Central  Google Scholar 

  • Zibaee I, Mahmood K, Esmaeily M, Bandani AR, Kristensen M (2018) Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran. J Appl Entomol 142:181–191

    CAS  Google Scholar 

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Acknowledgements

Authors are grateful to The Director, ICAR-IIHR and Head, Division of Crop Protection, ICAR-IIHR, for supporting and giving valuable suggestion throughout the study. The farmers of the visited places are greatly acknowledged for providing the inputs and co-operating throughout the study. We are also thankful to DST-SERB, New Delhi for financial funding through ECRA project (ECR/2017/000715).

Funding

The present work was funded by DST-SERB, Government of India, under ECRA (ECR/2017/000715).

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Authors and Affiliations

  1. Division of Crop Protection, ICAR-Indian Institute of Horticultural Research, Hessarghatta, Bengaluru, 560 089, India

    N. R. Prasannakumar, N. Jyothi & S. Saroja

  2. Division of Basic Sciences, ICAR-Indian Institute of Horticultural Research, Hessarghatta, Bengaluru, 560 089, India

    G. Ram Kumar

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  1. N. R. Prasannakumar
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  3. S. Saroja
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Correspondence to N. R. Prasannakumar.

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Prasannakumar, N.R., Jyothi, N., Saroja, S. et al. Relative toxicity and insecticide resistance of different field population of tomato leaf miner, Tuta absoluta (Meyrick). Int J Trop Insect Sci 41, 1397–1405 (2021). https://doi.org/10.1007/s42690-020-00334-1

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