Abstract
Zaprionus indianus was first reported in South America in 1999 and has rapidly colonised most of the continent since. Previous studies established Brazil as the sole entry point into the continent and the source of its expansion northward, but dispersal to the southern limit of its distribution in South America has not been studied so far. In the present work we extracted DNA of 127 individuals collected in north-eastern Argentina (South America) and amplified the COII mitochondrial gene in order to describe the mitochondrial genetic diversity and shed light about the invasion history of this species into this area. Our results suggest a single Z. indianus introduction wave from Brazil has recently occurred in north-eastern Argentina. In addition, by comparison with available sequences from other locations we can state that the invasive haplotypes present in Argentina are shared with those found in Brazilian populations and are phylogenetically closer to haplotypes from Africa than those found in India. This places Brazil as the sole recipient of the invasion to the American continent and its subsequent dispersion southwards.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bertheau C, Schuler H, Krumböck S, Arthofer W, Stauffer C (2011) Hit or miss in phylogeographic analyses: the case of the cryptic NUMTs. Mol Ecol Resour 11:1056–1059. https://doi.org/10.1111/j.1755-0998.2011.03050.x
Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659. https://doi.org/10.1046/j.1365-294x.2000.01020.x
Collura RV, Stewart C-B (1995) Insertions and duplications of mtDNA in the nuclear genomes of Old World monkeys and hominoids. Nature 378:485–489. https://doi.org/10.1038/378485a0
Commar LS, da Galego LGDC, Ceron CR, Carareto CMA (2012) Taxonomic and evolutionary analysis of Zaprionus indianus and its colonization of Palearctic and Neotropical regions. Genet Mol Biol 35:395–406. https://doi.org/10.1590/S1415-47572012000300003
David JR, Araripe LO, Bitner-Mathé BC, Capy P, Goñi B, Klaczko LB, Legout H, Martins MB, Vouidibio J, Yassin A, Moreteau B (2006) Quantitative trait analysis and geographic variability of natural populations of Zaprionus indianus, a recent invader in Brazil. Heredity 96(1):53–62. https://doi.org/10.1038/sj.hdy.6800753
Goñi B, Martinez ME, Techera G, Fresia P (2002) Increased frequencies of Zaprionus indianus Gupta, 1970 (Diptera, Drosophilidae) in Uruguay. Dros Inf Serv 85:75–80
Hall TA (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series. [London]: Information Retrieval Ltd., c1979–c2000, pp 95–98
Holle SG, Tran AK, Burkness EC, Ebbenga DN, Hutchison WD (2018) First Detections of Zaprionus indianus (Diptera: Drosophilidae) in Minnesota. J Entomol Sci 54:99–102. https://doi.org/10.18474/JES18-22
Lavagnino NJ, Carreira VP, Mensch J, Hasson E, Fanara JJ (2008): Geographic distribution and hosts of Zaprionus indianus (Diptera: Drosophilidae) in North-Eastern Argentina. Revista de la Sociedad Entomológica Argentina
Lavagnino NJ, Fanara JJ, Mensch J (2020) Comparison of overwintering survival and fertility of Zaprionus indianus (Diptera: Drosophilidae) flies from native and invaded ranges. J Therm Biol 87:102470. https://doi.org/10.1016/j.jtherbio.2019.102470
Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452. https://doi.org/10.1093/bioinformatics/btp187
Markow TA, Hanna G, Riesgo-Escovar JR, Tellez-Garcia AA, Richmond MP, Nazario-Yepiz NO, Laclette MRL, Carpinteyro-Ponce J, Pfeiler E (2014) Population genetics and recent colonization history of the invasive drosophilid Zaprionus indianus in Mexico and Central America. Biol Invasions 16:2427–2434. https://doi.org/10.1007/s10530-014-0674-5
Renkema JM, Miller M, Fraser H, Légaré JP, Hallett RH (2013) First records of Zaprionus indianus Gupta (Diptera: Drosophilidae) from commercial fruit fields in Ontario and Quebec, Canada. J Entomol Soc Ontario 144:125–130
Richly E, Leister D (2004) NUMTs in sequenced eukaryotic genomes. Mol Biol Evol 21(6):1081–1084. https://doi.org/10.1093/molbev/msh110
Robe LJ, Valente VL, Budnik M, Loreto ÉL (2005) Molecular phylogeny of the subgenus Drosophila (Diptera, Drosophilidae) with an emphasis on Neotropical species and groups: a nuclear versus mitochondrial gene approach. Mol Phylogenet Evol 36(3):623–640. https://doi.org/10.1016/j.ympev.2005.05.005
Rogers HH, Griffiths-Jones S (2012) Mitochondrial pseudogenes in the nuclear genomes of Drosophila. PLoS ONE 7:e32593. https://doi.org/10.1371/journal.pone.0032593
Sarswat M, Dewan S, Fartyal RS (2016) Mitochondrial DNA sequence variation in Drosophilid species (Diptera: Drosophilidae) along altitudinal gradient from Central Himalayan region of India. J Genet 95:357–367. https://doi.org/10.1007/s12041-016-0646-x
Tajima F (1989) The effect of change in population size on DNA polymorphism. Genetics 123:597–601
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680. https://doi.org/10.1093/nar/22.22.4673
van der Linde K, Steck GJ, Hibbard K, Birdsley JS, Alonso LM, Houle D (2006) First records of Zaprionus indianus (Diptera: Drosophilidae), a pest species on commercial fruits from Panama and the United States of America. Florida Entomol 89:402–404. https://doi.org/10.1653/0015-4040(2006)89%5b402:FROZID%5d2.0.CO;2
van der Linde K, Houle D, Spicer GS, Steppan SJ (2010) A supermatrix-based molecular phylogeny of the family Drosophilidae. Genet Res 92(1):25–38. https://doi.org/10.1017/S001667231000008X
Vilela CR (1999) Is Zaprionus indianus Gupta, 1970 (Diptera, Drosophilidae) currently colonizing the Neotropical region? Drosophila Inf Serv 82:37–39
Vilela CR, Teixeira EP, Stein CP (2001): Mosca-africana-do-figo, Zaprionus indianus (Diptera: Drosophilidae). Histórico e impacto das pragas introduzidas no Brasil Holos, Ribeirao Preto, Brazil, pp 48–52
Yassin A, Capy P, Madi-Ravazzi L, Ogereau D, David JR (2008) DNA barcode discovers two cryptic species and two geographical radiations in the invasive drosophilid Zaprionus indianus. Mol Ecol Resour 8:491–501. https://doi.org/10.1111/j.1471-8286.2007.02020.x
Yassin A, Borai F, Capy P, David JR, Elias E, Riad SA, Shalaby HG, Serour S, Abou-Youssef AY (2009) Evolutionary Genetics of Zaprionus. II. Mitochondrial DNA and chromosomal variation of the invasive drosophilid Zaprionus indianus in Egypt. Mitochondrial DNA 20:34–40. https://doi.org/10.1080/19401730902890042
Acknowledgements
We would like to thank the comments and suggestions of an anonymous reviewer. We would also like to thank Silvia Pietrokovsky for assistance with English language in earlier drafts. This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (Argentina, grant number PICT 2016-2256) and by the Universidad de Buenos Aires (Argentina, grant numbers UBACyT 20020100100482 and UBACyT 20020170200061BA). NJL, JJF and MSR are members of Carrera del Investigador Científico of CONICET (Argentina), MI and GR are recipients of a scholarship from CONICET (Argentina) and LFG is recipient of a scholarship from the Universidad de Buenos Aires.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Fernandez Goya, L., Imberti, M., Rodriguero, M.S. et al. Mitochondrial genetic diversity of the invasive drosophilid Zaprionus indianus (Diptera: Drosophilidae) in South America. Biol Invasions 22, 3481–3486 (2020). https://doi.org/10.1007/s10530-020-02342-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10530-020-02342-1