Skip to main content
SpringerLink
Log in

A novel electrochemical biosensor based on peptidoglycan and platinum-nickel-copper nano-cube for rapid detection of Gram-positive bacteria

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A novel non-enzyme electrochemical biosensor for the rapid detection of Gram-positive bacteria has been constructed that relys on a stable and efficient combination between the peptidoglycan layer and platinum-nickel-copper nanocubes (Pt-Ni-Cu NCs). Briefly, bacteria were first captured by a specific antibody. Then, the electrochemical signal materials (Pt-Ni-Cu NCs) were bound to the bacteria peptidoglycan layer using specific structural and surface features. The rapid and sensitive bacterial detection was then achieved using intrinsic electrochemical characteristics and superoxidase-like activity of the Pt-Ni-Cu NCs. Moreover, the nature of peptidoglycan covering the whole bacteria provided the premise for signal amplification. Under optimal conditions, the electrochemical signal variation was proportional to the concentration of bacteria ranging from 1.5 × 102 to 1.5 × 108 CFU/mL with a detection limit of 42 CFU/mL using a working potential of − 0.4 V. This electrochemical biosensor has been successfully applied to detect bacteria concentrations in urine samples, and the recoveries range from 90.4 to 107%. The proposed biosensor could be applied for broad-spectrum detection of Gram-positive bacteria since most Gram-positive bacteria possess a thick peptidoglycan layer. The developed electrochemical biosensing strategy might be used as a potential tool for clinical pathogenic bacteria detection and point-of-care testing (POCT).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Al Tall Y, Abualhaijaa A, Alsaggar M, Almaaytah A, Masadeh M, Alzoubi KH (2019) Design and characterization of a new hybrid peptide from LL-37 and BMAP-27. Infect Drug Resist 12:1035–1045. https://doi.org/10.2147/IDR.S199473

    Article  PubMed  PubMed Central  Google Scholar 

  2. Goad B, Harris LK (2018) Identification and prioritization of macrolideresistance genes with hypothetical annotation inStreptococcus pneumoniae. Bioinformation 14(9):488–498. https://doi.org/10.6026/97320630014488

    Article  PubMed  PubMed Central  Google Scholar 

  3. Koirala B, Phillips NR, Tal-Gan Y (2019) Unveiling the importance of amide protons in CSP:ComD interactions in Streptococcus pneumoniae. ACS Med Chem Lett 10(6):880–886. https://doi.org/10.1021/acsmedchemlett.9b00038

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Panda PS, Chaudhary U, Dube SK (2016) Comparison of four different methods for detection of biofilm formation by uropathogens. Indian J Pathol Microbiol 59(2):177–179. https://doi.org/10.4103/0377-4929.182013

    Article  PubMed  Google Scholar 

  5. Borghei Y, Hosseini M, Ganjali MR (2017) Fluorometric determination of microRNA via FRET between silver nanoclusters and CdTe quantum dots. Microchim Acta 184(12):4713–4721. https://doi.org/10.1007/s00604-017-2512-9

    Article  CAS  Google Scholar 

  6. Zhao H, Shu G, Zhu J, Fu Y, Gu Z, Yang D (2019) Persistent luminescent metal-organic frameworks with long-lasting near infrared emission for tumor site activated imaging and drug delivery. Biomaterials 217:e119332. https://doi.org/10.1016/j.biomaterials.2019.119332

    Article  CAS  Google Scholar 

  7. Han DB, Yan YR, Wang JM et al (2019) An enzyme-free electrochemiluminesce aptasensor for the rapid detection of Staphylococcus aureus by the quenching effect of MoS2-PtNPs-vancomycin to S2O82−/O2 system. Sensors Actuators B Chem 288:586–593. https://doi.org/10.1016/j.bios.2013.03.084

    Article  CAS  Google Scholar 

  8. Zhang L, Deng H, Yuan R, Yuan Y (2019) Electrochemical lead(II) biosensor by using an ion-dependent split DNAzyme and a template-free DNA extension reaction for signal amplification. Microchim Acta 186(11). https://doi.org/10.1007/s00604-019-3857-z

  9. Asif M, Haitao W, Shuang D, Aziz A, Zhang G, Xiao F, Liu H (2017) Metal oxide intercalated layered double hydroxide nanosphere: with enhanced electrocatalyic activity towards H2O2 for biological applications. Sensors Actuators B Chem 239:243–252. https://doi.org/10.1016/j.snb.2016.08.010

    Article  CAS  Google Scholar 

  10. Guo B, Wen B, Cheng W, Zhou X, Duan X, Zhao M, Xia Q, Ding S (2018) An enzyme-free and label-free surface plasmon resonance biosensor for ultrasensitive detection of fusion gene based on DNA self-assembly hydrogel with streptavidin encapsulation. Biosens Bioelectron 112:120–126. https://doi.org/10.1016/j.bios.2018.04.027

    Article  CAS  PubMed  Google Scholar 

  11. Asif M, Aziz A, Azeem M, Wang Z, Ashraf G, Xiao F, Chen X, Liu H (2018) A review on electrochemical biosensing platform based on layered double hydroxides for small molecule biomarkers determination. Adv Colloid Interf Sci 262:21–38. https://doi.org/10.1016/j.cis.2018.11.001

    Article  CAS  Google Scholar 

  12. Asif M, Liu H, Aziz A, Wang H, Wang Z, Ajmal M, Xiao F, Liu H (2017) Core-shell iron oxide-layered double hydroxide: high electrochemical sensing performance of H2O2 biomarker in live cancer cells with plasma therapeutics. Biosens Bioelectron 97:352–359. https://doi.org/10.1016/j.bios.2017.05.057

    Article  CAS  PubMed  Google Scholar 

  13. Luo C, Tang H, Cheng W, Yan L, Zhang D, Ju H, Ding S (2013) A sensitive electrochemical DNA biosensor for specific detection of Enterobacteriaceae bacteria by Exonuclease III-assisted signal amplification. Biosens Bioelectron 48:132–137. https://doi.org/10.1016/j.bios.2013.03.084

    Article  CAS  PubMed  Google Scholar 

  14. Maduraiveeran G, Sasidharan M, Ganesan V (2018) Electrochemical sensor and biosensor platforms based on advanced nanomaterials for biological and biomedical applications. Biosens Bioelectron 103:113–129. https://doi.org/10.1016/j.bios.2017.12.031

    Article  CAS  PubMed  Google Scholar 

  15. Xu L, Liu Z, Lei S, Huang D, Zou L, Ye B (2019) A sandwich-type electrochemical aptasensor for the carcinoembryonic antigen via biocatalytic precipitation amplification and by using gold nanoparticle composites. Microchim Acta 186(7):473. https://doi.org/10.1007/s00604-019-3542-2

    Article  CAS  Google Scholar 

  16. Jiang HL, Ma X, Wang L, Zhang Q (2019) Switching on photocatalysis of metal-organic frameworks by engineering structural defects. Angew Chem Int Ed Eng 131:12303–12307. https://doi.org/10.1002/anie.201907074

    Article  CAS  Google Scholar 

  17. Sun X, Tao Y, Du Y, Ding W, Chen C, Ma X (2019) Metal organic framework HKUST-1 modified with carboxymethyl-β-cyclodextrin for use in improved open tubular capillary electrochromatographic enantioseparation of five basic drugs. Microchim Acta 186(7):462–469. https://doi.org/10.1007/s00604-019-3584-5

    Article  CAS  Google Scholar 

  18. Ohtani M, Muraoka T, Okimoto Y, Kobiro K (2017) Rapid one-pot solvothermal batch synthesis of porous nanocrystal assemblies composed of multiple transition-metal elements. Inorg Chem 56(19):11546–11551. https://doi.org/10.1021/acs.inorgchem.7b01192

    Article  CAS  PubMed  Google Scholar 

  19. Li W, Chen B, Zhang H, Sun Y, Wang J, Zhang J, Fu Y (2015) BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury(II) ions. Biosens Bioelectron 66:251–258. https://doi.org/10.1016/j.bios.2014.11.032

    Article  CAS  PubMed  Google Scholar 

  20. Xu X, Zhang X, Sun H, Yang Y, Dai X, Gao J, Li X, Zhang P, Wang HH, Yu NF, Sun SG (2014) Synthesis of Pt-Ni alloy nanocrystals with high-index facets and enhanced electrocatalytic properties. Angew Chem Int Ed Eng 53(46):12522–12527. https://doi.org/10.1002/anie.201406497

    Article  CAS  Google Scholar 

  21. Mostafa S, Behafarid F, Croy JR, Ono LK, Li L, Yang JC, Frenkel AI, Cuenya BR (2010) Shape-dependent catalytic properties of Pt nanoparticles. J Am Chem Soc 132(44):15714–15719. https://doi.org/10.1021/ja106679z

    Article  CAS  PubMed  Google Scholar 

  22. Jin L, Meng Z, Zhang Y, Cai S, Zhang Z, Li C, Shang L, Shen Y (2017) Ultrasmall Pt nanoclusters as robust peroxidase mimics for colorimetric detection of glucose in human serum. ACS Appl Mater Interfaces 9(11):10027–10033. https://doi.org/10.1021/acsami.7b01616

    Article  CAS  PubMed  Google Scholar 

  23. Mu X, Wang J, Li Y, Xu F, Long W, Ouyang L, Liu H, Jing Y, Wang J, Dai H, Liu Q, Sun Y, Liu C, Zhang XD (2019) Redox trimetallic nanozyme with neutral environment preference for brain injury. ACS Nano 13(2):1870–1884. https://doi.org/10.1021/acsnano.8b08045

    Article  CAS  PubMed  Google Scholar 

  24. Asif M, Aziz A, Wang Z, Ashraf G, Wang J, Luo H, Chen X, Xiao F, Liu H (2019) Hierarchical CNTs@CuMn layered double hydroxide nanohybrid with enhanced electrochemical performance in H2S detection from live cells. Anal Chem 91(6):3912–3920. https://doi.org/10.1021/acs.analchem.8b04685

    Article  CAS  PubMed  Google Scholar 

  25. Pei F, Wang P, Ma E, Yu H, Gao C, Yin H, Li Y, Liu Q, Dong Y (2018) A sandwich-type amperometric immunosensor fabricated by Au@Pd NDs/Fe2+-CS/PPy NTs and Au NPs/NH2-GS to detect CEA sensitively via two detection methods. Biosens Bioelectron 122:231–238. https://doi.org/10.1016/j.bios.2018.09.065

    Article  CAS  PubMed  Google Scholar 

  26. Babamiri B, Bahari D, Salimi A (2019) Highly sensitive bioaffinity electrochemiluminescence sensors: recent advances and future directions. Biosens Bioelectron 142:111530. https://doi.org/10.1016/j.bios.2019.111530

    Article  CAS  PubMed  Google Scholar 

  27. Cabeen MT, Jacobs-Wagner C (2005) Bacterial cell shape. Nat Rev Microbiol 3(8):601–610. https://doi.org/10.1038/nrmicro1205

    Article  CAS  PubMed  Google Scholar 

  28. Johnson JW, Fisher JF, Mobashery S (2013) Bacterial cell-wall recycling. Ann N Y Acad Sci 1277:54–75. https://doi.org/10.1111/j.1749-6632.2012.06813.x

    Article  CAS  PubMed  Google Scholar 

  29. Wu R, Ma Y, Pan J, Lee SH, Pan G (2017) Efficient capture, rapid killing and ultrasensitive detection of bacteria by a nano-decorated multi-functional electrode sensor. Biosens Bioelectron 101:52–59. https://doi.org/10.1016/j.bios.2017.10.003

    Article  CAS  PubMed  Google Scholar 

  30. Kashyap DR, Wang M, Liu LH, Boons GJ, Gupta D, Dziarski R (2011) Peptidoglycan recognition proteins kill bacteria by activating protein-sensing two-component systems. Nat Med 17(6):676–683. https://doi.org/10.1038/nm.2357

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Turner RD, Hurd AF, Cadby A, Hobbs JK, Foster SJ (2013) Cell wall elongation mode in Gram-negative bacteria is determined by peptidoglycan architecture. Nat Commun 4:1496–1503. https://doi.org/10.1038/ncomms2503

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Girardin SE, Boneca IG, Carneiro LA et al (2003) Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science 300(5625):1584–1587. https://doi.org/10.1126/science.1084677

    Article  CAS  PubMed  Google Scholar 

  33. Fang G, Li W, Shen X, Perez-Aguilar JM, Chong Y, Gao X, Chai Z, Chen C, Ge C, Zhou R (2018) Differential Pd-nanocrystal facets demonstrate distinct antibacterial activity against Gram-positive and Gram-negative bacteria. Nat Commun 9(1):129–137. https://doi.org/10.1038/s41467-017-02502-3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Ye JS, Chen C, Lee C (2015) Pd nanocube as non-enzymatic glucose sensor. Sensors Actuators B Chem 208:569–574. https://doi.org/10.1016/j.snb.2014.11.091

    Article  CAS  Google Scholar 

  35. Niroula J, Premaratne G, Ali Shojaee S, Lucca DA, Krishnan S (2016) Combined covalent and noncovalent carboxylation of carbon nanotubes for sensitivity enhancement of clinical immunosensors. Chem Commun (Camb) 52(88):13039–13042. https://doi.org/10.1039/c6cc07022a

    Article  CAS  Google Scholar 

  36. Wu Y, Wang D, Chen X, Zhou G, Yu R, Li Y (2013) Defect-dominated shape recovery of nanocrystals: a new strategy for trimetallic catalysts. J Am Chem Soc 135(33):12220–12223. https://doi.org/10.1021/ja4068063

    Article  CAS  PubMed  Google Scholar 

  37. Feng X, Zhang Y, Zhou J, Li Y, Chen S, Zhang L, Ma Y, Wang L, Yan X (2015) Three-dimensional nitrogen-doped graphene as an ultrasensitive electrochemical sensor for the detection of dopamine. Nanoscale 7(6):2427–2432. https://doi.org/10.1039/c4nr06623e

    Article  CAS  PubMed  Google Scholar 

  38. Wei Y, Li X, Sun X, Ma H, Zhang Y, Wei Q (2017) Dual-responsive electrochemical immunosensor for prostate specific antigen detection based on Au-CoS/graphene and CeO2/ionic liquids doped with carboxymethyl chitosan complex. Biosens Bioelectron 94:141–147. https://doi.org/10.1016/j.bios.2017.03.001

    Article  CAS  PubMed  Google Scholar 

  39. Aziz A, Asif M, Azeem M, Ashraf G, Wang Z, Xiao F, Liu H (2019) Self-stacking of exfoliated charged nanosheets of LDHs and graphene as biosensor with real-time tracking of dopamine from live cells. Anal Chim Acta 1047:197–207. https://doi.org/10.1016/j.aca.2018.10.008

    Article  CAS  PubMed  Google Scholar 

  40. Asif M, Aziz A, Wang H, Wang Z, Wang W, Ajmal M, Xiao F, Chen X, Liu H (2019) Superlattice stacking by hybridizing layered double hydroxide nanosheets with layers of reduced graphene oxide for electrochemical simultaneous determination of dopamine, uric acid and ascorbic acid. Microchim Acta 186(2):61–71. https://doi.org/10.1007/s00604-018-3158-y

    Article  CAS  Google Scholar 

Download references

Funding

This study was financially supported by the National Natural Science Foundation of China (81873980, 81873972, 21804015), the National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202), and the Natural Science Foundation Project of CQ (cstc2018jcyjAX0201, cstc2018jcyjAX0251).

Author information

Author notes

  1. Daobin Han and Yurong Yan contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China

    Daobin Han, Yurong Yan, Xintong Bian, Jianmin Wang, Min Zhao, Xiaolei Duan, Liangsheng Kong & Shijia Ding

  2. Department of Laboratory Medicine, The Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou, China

    Xiaolei Duan

  3. The Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

    Wei Cheng

Authors
  1. Daobin Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yurong Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xintong Bian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jianmin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Min Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaolei Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Liangsheng Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wei Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shijia Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shijia Ding.

Ethics declarations

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 210 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Han, D., Yan, Y., Bian, X. et al. A novel electrochemical biosensor based on peptidoglycan and platinum-nickel-copper nano-cube for rapid detection of Gram-positive bacteria. Microchim Acta 187, 607 (2020). https://doi.org/10.1007/s00604-020-04581-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-020-04581-4

Keywords

Search

Navigation