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Identification and nomenclature of Albugo candida pathotypes of Indian origin causing white rust disease of rapeseed-mustard

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Abstract

The highly variable nature of Albugo candida pathogen in terms of number of races/pathotypes poses a challenge in the proper identification. Therefore, in this study characterization and identification of 33 A. candida isolates of Indian origin have been done on the basis of molecular and host differentials studies. Molecular characterization using ITS and COX2 gene yielded 1250 bp and 650 bp bands while maximum parsimony trees showed 15 and 13 clusters from 33 A. candida isolates, respectively. On the basis of pathogenicity reactions on 19 host differentials of different Brassica spp., 3 major groups have been identified as Group Ia, Ib, Ic, Id, Ie, II, and III. Group Ia was the pathotype of Brassica juncea, Ib- B. juncea cv. Cutlass, Ic- B. juncea cv. Donskaja, Id- a different B. juncea pathotype that showed disease reaction on B. oleracea, Ie- a different B. juncea pathotype that showed disease reaction on B. carinata cv. Kiran, Gropu II- B. rapa var. Toria, Group III- B. rapa pathotypes, respectively. Finally, from 33 A. candida isolates a total of 21 pathotypes have been identified on the basis of combined grouping of pathogenicity reactions on host differentials and ITS and COX2 gene sequence analysis and new nomenclature has been given as per the International standard, out of which 15 pathotypes showed more virulence to B. juncea that named as AC2–1 to AC2–15, 6 pathotypes showed virulence to B. rapa that named as AC7–1 to AC7–6. A. candida pathotypes identified from different geographical regions of India could be utilized for the identification and deployment of the resistant cultivar in major mustard growing areas.

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Acknowledgments

The present investigation is a part of Ph. D Thesis of Mr. Devanshu Dev that was conducted at Oilseed Pathology Laboratory, Department of Plant Pathology, GBPUA&T, Pantnagar, Uttarakhand, India. Indo-UK (DBT) Project, Govt. of India is duly acknowledged for providing financial assistance. The first author is thankful to the Council of Scientific & Industrial Research (CSIR), Govt. of India for providing Junior Research Fellowship (JRF) and Senior Research Fellowship (SRF) during the Ph.D. degree program. I would like to express my sincere thanks to Dr. Deepak Pental (Ex-VC, University of Delhi) for providing me host differentials seeds to conduct my experiments.

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  1. Department of Plant Pathology, College of Agriculture, G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Devanshu Dev, A. K. Tewari, Pooja Upadhyay & G. R. Daniel

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  1. Devanshu Dev
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  2. A. K. Tewari
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  3. Pooja Upadhyay
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  4. G. R. Daniel
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Correspondence to Devanshu Dev.

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Author A (Mr. Devanshu Dev) declares that I have no conflict of interest. Author B (Dr. A. K. Tewari) declares that I have no conflict of interest.

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Dev, D., Tewari, A.K., Upadhyay, P. et al. Identification and nomenclature of Albugo candida pathotypes of Indian origin causing white rust disease of rapeseed-mustard. Eur J Plant Pathol 158, 987–1004 (2020). https://doi.org/10.1007/s10658-020-02135-1

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