Skip to main content
SpringerLink
Log in

Morphometric Analysis of the Internal Ultrastructure of Mitochondria of Muscle Tissue in Horsehair Worm Gordionus alpestris (Nematomorpha)

  • Published:
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract—

Internal ultrastructure of the muscle tissue mitochondria of horsehair worm Gordionus alpestris (Nematomorpha) was studied using morphometry. Surface area of the inner mitochondrial membrane per unit of the mitochondrial volume, or surface density of the inner mitochondrial membrane, was measured as a main morphometric parameter. The surface density of the inner mitochondrial membrane of the G. alpestris muscle tissue was compared to the respective parameter of the skeletal and cardiac muscle mitochondria. The surface density of the inner mitochondrial membrane of the worm was close to the surface density values of the cardimyocytes of 3-month-old mice and Wistar rats and was slightly higher than the surface density of mitochondria from the skeletal muscle of 3-month-old mice. The functional significance of the well-developed system of mitochondrial membranes of extended mitochondria of the horsehair worm is discussed as a structure necessary to ensure effective functioning of the circomyarian conntractile apparatus in the muscle tissue of the horsehair worm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig 3.
Fig 4.

Similar content being viewed by others

REFERENCES

  1. Vays V.B., Vangeli I.M., Eldarov C.M., Bakeeva L.E., Efeykin B.D. 2019. Mitochondria in obliquely striated muscles of the horsehair worm Gordionus alpestris (Nematomorpha, Gordioidea) with structural organization typical of cells with energy-intensive processes. Biochemistry (Moscow). 84 (1), 56–61.

    Article  CAS  Google Scholar 

  2. Lanzavecchia G., Valvassory R., Magda de Eguileor, Lanzavecchia P. 1979. Three-dimensional reconstruction of the contractile system of the Nematomorpha muscle fiber. J. Ultrastruct. Res. 66, 201–223.

    Article  CAS  Google Scholar 

  3. Restelli M. A., de Villalobos L.C., Fernanda Z. 2002. Ultrastructural description of the musculature, the intraepidermal nervous system, and their interrelation in Pseudochordodes bedriagae (Nevatomorpha). Cell Tissue Res. 308, 299–306.

    Article  Google Scholar 

  4. Weibel E.R. 1979. Stereological methods. Practical methods for biological morphometry. London, Academic Press.

    Google Scholar 

  5. Gundersen H.J., Bendtsen T.F., Korbo L., Marcussen N., Møller A., Nielsen K., Nyengaard J.R., Pakkenberg B., Sørensen F.B., Vesterby A. 1989. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS. 96 (5), 379–394.

    Article  Google Scholar 

  6. Mandarim-de-Lacerda C.A. 2003. Stereological tools in biomedical research. An. Acad. Bras. Cienc. 75 (4), 469–486.

    Article  Google Scholar 

  7. Shinderite V.S., Yasaitis A.A. 1984. Effects of energy metabolism inhibitors on the morphometric parameters and spatial structure of mitochondria in L cells. Tsitologya (Rus.). 26, 666–671.

    CAS  Google Scholar 

  8. Eldarov C.M., Vays V.B., Vangeli I.M., Kolosova N.G., Bakeeva L.E. 2015. Morphometric examination of mitochondrial ultrastructure in aging cardiomyocytes. Biochemistry (Moscow).80 (5), 604–609.

    Article  CAS  Google Scholar 

  9. Nepomnyashchikh L.M., Lushnikova E.L., Molodykh N.A., Klinnikova M.G., Molodykh O.P. 2011. Ultrastructure and stereology of cardiomyocytes in the development of regenerative and plastic myocardial insufficiency during ontogeny. Bull. Exp. Biol. Med.151 (1), 88–94.

    Article  CAS  Google Scholar 

  10. Mccallister B.D., Brown A.L. 1965. A quantitative morphological study of the mitochondria in experimental cardiac hypertrophy. Lab. Invest. 14, 692–700.

    CAS  PubMed  Google Scholar 

  11. Cieciura L., Rydzynski K., Klitonczyk W. 1979. Stereologic studies on mitochondrial configuration in different organs of the rat. Cell Tissue Res. 196, 347–360.

    Article  CAS  Google Scholar 

  12. Dogel V.A. 1975. Zoologia bespozvonochnykh (Zoology of invertebrates). M., Vysshaya Shkola.

    Google Scholar 

  13. Bakeeva L.E., Skulachev V.P., Chentsov Yu.S. 1977. Mitochonrial reticulum: Structure and possible functions of the new type intracellular organelles in muscle tissue. Vestnik Mosc. Univ., Ser. Biologia (Rus.). 3, 23–38.

    Google Scholar 

  14. Bakeeva L.E., Chentsov Yu.S., Skulachev V.P. 1978. Mitochondrial framework (reticulum mitochondriale) in rat diaphragm muscle. Biochim. Biophys. Acta.501, 349–369.

    Article  CAS  Google Scholar 

  15. Bakeeva L.E., Chentsov Yu.S., Skulachev V.P. 1982. Intermitochondrial contacts in cardiomyocytes. Tsitologia (Rus.). 24 (2), 161–166.

    CAS  Google Scholar 

  16. Bakeeva L.E., Chentsov Yu.S., Skulachev V.P. 1983. Intermitochondrial contacts in myocardiocytes. J. Mol. Cell. Cardiol. 15, 413–420.

    Article  CAS  Google Scholar 

  17. Zavarzin A.A. 1985. Osnovy sravnitel’noi gistologii (Fundamentals of comparative histology). L., Izd. Leningradskogo Universiteta, p. 321.

    Google Scholar 

Download references

ACKNOWLEDGMENTS

The authors thank Dr. V.V. Aleshin for the assistance in the data interpretation and preparation of the manuscript. The work was supported by the Russian Foundation for Basic Research (project no. 18-34-00413).

Author information

Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Moscow Lomonosov State University, 119991, Moscow, Russia

    C. M. Eldarov, V. B. Vays, I. M. Vangeli, O. A. Averina & L. E. Bakeeva

  2. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071, Moscow, Russia

    B. D. Efeykin

Authors
  1. C. M. Eldarov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. B. Vays
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. M. Vangeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O. A. Averina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. D. Efeykin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. E. Bakeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. E. Bakeeva.

Ethics declarations

The authors declare that they have no conflict of interest.

All procedures were performed in accordance with the protocol approved by the local bioethics comittee IC VEK OOO “Research Institute of Mitoengineering MSU” (resolution no. 67 of 28.04.2015), European Communities Council Directive (November 24, 1986; 86/609/EEC) and the Declaration on humane treatment of animals.

Additional information

Translated by Ch. Eldarov

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Eldarov, C.M., Vays, V.B., Vangeli, I.M. et al. Morphometric Analysis of the Internal Ultrastructure of Mitochondria of Muscle Tissue in Horsehair Worm Gordionus alpestris (Nematomorpha). Biochem. Moscow Suppl. Ser. A 14, 255–259 (2020). https://doi.org/10.1134/S199074782002004X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S199074782002004X

Keywords:

Search

Navigation