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Comparative Leaf Proteomics of Brassica napus Genotypes with Distinctive Levels of Early Cold Acclimation

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Abstract

Rapeseed/canola (Brassica napus L.) is considered amongst the “big four” oilseed crops with varying degrees of cold tolerance. Differential protein expression of cold acclimated young leaves of two canola genotypes, Sari Gul (spring type cold-sensitive) and Zarfam (winter type cold-tolerant), were determined via two dimensional polyacrylamide gel electrophoresis. Seedling leaves were cold acclimated by temperature regime and collected before and after cold treatment at 4 and − 4 °C after 24 h, proteins were isolated and separated on 2-DE. Gel analysis of 651 protein spots revealed that 19 protein spots were significantly different between the two conditions in response to early cold acclimation. Following nanoflow liquid chromatography–tandem mass spectrometry, 19 protein sequences were identified in gel spots, which were reported for the first time in response to early cold acclimation in B. napus and nearly half of them were associated with various aspects of chloroplast physiology; suggesting that the cold stress response of B. napus is achieved, at least partly, by regulation of chloroplast function. Our work has provided novel insights into the plant response to cold stress and should pave the way for future studies towards functional analysis of candidate genes in cold response.

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Acknowledgments

The authors thank Iran National Science Foundation (INSF) (grant number: 95814286) for financial support. We also would like to thank Mehdi Amini at Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran, for being instrumental.

Funding

This work was supported by the Iran National Science Foundation (INSF) [Grant number: 95814286, 2017-2019.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Evin, Tehran, Iran

    Khazar Edrisi Maryan & Naser Farrokhi

  2. Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran

    Khazar Edrisi Maryan, Habibollah Samizadeh Lahiji & Hassan Hasani Komeleh

  3. Department of Molecular Sciences, Macquarie University, North Ryde, NSW, 2109, Australia

    Paul A. Haynes & Sara Hamzelou

Authors
  1. Khazar Edrisi Maryan
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  2. Habibollah Samizadeh Lahiji
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  3. Naser Farrokhi
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  4. Paul A. Haynes
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  5. Sara Hamzelou
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  6. Hassan Hasani Komeleh
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Contributions

Khazar Edrisi Maryan carried out the plant culture, protein isolation, 2D-PAGE, spot analysis, and preparation of manuscript first draft. Habibollah Samizadeh Lahiji supervised Khazar Edrisi Maryan and designed the initial setup of the experiments. Naser Farrokhi supervised Khazar Edrisi Maryan on protein isolation, 2D-PAGE, bioinformatics analysis of the corresponding genes and promoters. Naser Farrokhi edited and commented on the draft and made it ready to be submitted to the journal. Protein identification by tandem mass spectrometry was carried out by Sara Hamzelou under the supervision of Paul A. Haynes. Hassan Hasani Komeleh advised Khazar Edrisi Maryan on plant culture and reviewed the manuscript.

Corresponding authors

Correspondence to Habibollah Samizadeh Lahiji or Naser Farrokhi.

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Key Message

• Cold acclimation is different between the cold tolerant vs cold sensitive genotypes that can be seen through changes of protein profiles.

• The highly affected cellular organelle in response to cold stress is chloroplast as reflected in expression changes that are reported in the corresponding proteins.

• Cold stress has pronounced effects on metabolic pathways.

• The expressions of corresponding genes to changed proteins appear to be in control of plant hormones, mainly methyl jasmonate and abscisic acid as determined via analysis of cis-elements within the promoters.

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Maryan, K.E., Lahiji, H.S., Farrokhi, N. et al. Comparative Leaf Proteomics of Brassica napus Genotypes with Distinctive Levels of Early Cold Acclimation. Plant Mol Biol Rep 39, 317–334 (2021). https://doi.org/10.1007/s11105-020-01249-4

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