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Evaluation of Phytotoxicity and Mutagenicity of Novel DMAEMA-Containing Gene Carriers

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Abstract—The use of novel carriers for gene delivery has been rapidly growing; thus, investigation of potential phytotoxic and mutagenic action of gene delivery carriers is important for preventing their negative side effects. We found that poly-DMAEMA carriers used in a 0.0025% dose exhibited a weak cytotoxic effect towards Allium cepa plants. In a higher dose (0.025%), they slightly (by 26–55%) increased the level of catalase activity but did not affect the level of superoxide dismutase activity and malondialdehyde content in roots of A. cepa. Results of the ana-telophase test in A. cepa demonstrated no genotoxic activity of the polymeric carriers used in a 0.0025% concentration and the higher dose (0.025%). Slight genotoxic activity was detected only for BGP24 and BGP26, PEG-containing poly-DMAEMA carriers, used in 0.025% concentration. The Ames test (–S9 and +S9) revealed no mutagenic potential of the DMAEMA-based polymers. Thus, low phytotoxicity and no mutagenicity of novel polymeric carriers suggest their potential as promising nanocarriers for gene delivery into plant cells.

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ACKNOWLEDGMENTS

We thank L.S. Bodnar, PhD, Associate Professor (Department of Genetics and Biotechnology, Ivan Franko National University of Lviv) for advice and technical support in conducting the Ames test.

Funding

This work was carried out with the financial support of the grant of the target complex interdisciplinary research program of the National Academy of Sciences of Ukraine “Molecular and Cellular Biotechnology for the Needs of Medicine, Industry, and Agriculture” for 2015–2019 (grant no. 0115U004198).

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Authors and Affiliations

  1. Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005, Lviv, Ukraine

    N. Finiuk & R. Stoika

  2. Ivan Franko National University of Lviv, 79005, Lviv, Ukraine

    N. Finiuk, N. Romanyuk, O. Terek & R. Stoika

  3. Lviv Polytechnic National University, 79013, Lviv, Ukraine

    N. Mitina & A. Zaichenko

  4. Institute of Ecology of the Carpathians, National Academy of Sciences of Ukraine, 79026, Lviv, Ukraine

    O. Lobachevska

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  1. N. Finiuk
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  2. N. Romanyuk
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Statement on the welfare of animals. Bioethical examination of experiments with laboratory animals was carried out at the Faculty of Biology of Ivan Franko National University of Lviv, protocol, no. 11052018 from May 15, 2018.

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Translated by K. Lazarev

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Finiuk, N., Romanyuk, N., Mitina, N. et al. Evaluation of Phytotoxicity and Mutagenicity of Novel DMAEMA-Containing Gene Carriers. Cytol. Genet. 54, 437–448 (2020). https://doi.org/10.3103/S0095452720050096

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