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Sustainable Lipase Production by Diutina rugosa NRRL Y-95 Through a Combined Use of Agro-Industrial Residues as Feedstock

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Abstract

The potential use of alternative culture media towards the development of a sustainable bioprocess to produce lipases by Diutina rugosa is clearly demonstrated. First, a synthetic medium containing glucose, peptone, yeast extract, oleic acid, and ammonium sulfate was proposed, with lipase activity of 143 U/L. Then, alternative culture media formulated with agro-industrial residues, such as molasses, corn steep liquor (CSL), and olive mill waste (OMW), were investigated. An experimental design was conducted, and only CSL concentration was found to have a positive effect in lipase production. The highest lipase activity (561 U/L) was produced on a mixture of molasses (5 g/L), CSL (6 g/L), OMW (0.5% v/v), 0.5 g/L of ammonium sulfate, and 3 g/L of peptone at 24 h of cultivation. Lipase production was also carried out in a 1-L bioreactor leading to a slightly higher lipase activity at 24 h of cultivation. The semi-purified enzyme exhibits an optimum temperature and pH of 40 °C and 7.0, respectively. Finally, the media cost per unit of lipase produced (UPC) was influenced by the medium components, specially by the inducer used. The lowest UPC was obtained when the agro-industrial residues were combined and used at the improved concentrations.

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Funding

The study is funded by CAPES, CNPq, and FUNCAP (from Brazil) for the financial support that made this work possible. In addition, the study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, the BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte, and the Project LIGNOZYMES (POCI-01-0145-FEDER-029773).

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici Bloco 709, Fortaleza, 60440-900, Brazil

    Maria de Fátima M. de Freitas, Lucas S. Cavalcante & Luciana R. B. Gonçalves

  2. Centre of Biological Engineering (CEB), University of Minho, 4710-057, Braga, Portugal

    Eduardo J. Gudiña, Sara C. Silvério & Lígia R. Rodrigues

  3. Departamento de Engenharia de Alimentos, Universidade Federal do Ceará, Campus do Pici, Bloco 858,, Fortaleza, 60440-900, Brazil

    Sueli Rodrigues

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  1. Maria de Fátima M. de Freitas
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de Freitas, M.d.F.M., Cavalcante, L.S., Gudiña, E.J. et al. Sustainable Lipase Production by Diutina rugosa NRRL Y-95 Through a Combined Use of Agro-Industrial Residues as Feedstock. Appl Biochem Biotechnol 193, 589–605 (2021). https://doi.org/10.1007/s12010-020-03431-6

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