Abstract
Different carbon sources lead to differential acarbose production in Actinoplanes. To uncover the underlying differentiation in the context of genes and pathways, we performed transcriptome sequencing of Actinoplanes utahensis ZJB-03852 grown on different saccharides, such as glucose, maltose, or the saccharide complex consisting of glucose plus maltose. The differentially expressed genes were classified into GO (gene ontology) terms and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways for functional annotations. Key enriched modules were uncovered. Our data revealed that both maltose and its complex with glucose gave improved acarbose titer. Sugar transportation, cytochrome oxidase, protein synthesis and amino acid metabolism modules were enriched under the saccharide complex condition, while ferritin metabolism gene expressions were enriched in the glucose medium. Our results provided the foundation for uncovering the mechanism of carbon source on acarbose production in A. utahensis.
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Abbreviations
- ACN:
-
Acetonitrile
- CDW:
-
Cell dry weight
- DEG:
-
Differentially expressed genes
- FPKM:
-
Fragments per kilobase per million mapped reads
- Glc:
-
A. utahensis ZJB-03852 cultured with 80 g/L glucose as the carbon source
- GM:
-
A. utahensis ZJB-03852 cultured with 40 g/L glucose and 40 g/L maltose as the carbon source
- GO:
-
Gene ontology
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- Mal:
-
A. utahensis ZJB-03852 cultured with 80 g/L maltose as the carbon source
- NRPS:
-
Non-ribosomal peptide synthetase
- PKS:
-
Polyketide synthase
- PPI:
-
Protein–protein interaction
- T2DM:
-
Type 2 diabetes mellitus
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Acknowledgements
This work was supported by the Natural Science Foundation of China (No. 21476209, No. 218782749), Natural Science Foundation of Zhejiang Province (No. LQ18C010005).
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CW and YW designed the study and drafted the manuscript, LS performed the experiment, YW and YZ revised the manuscript. All authors read and approved the final manuscript.
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Weng, CY., Shi, LZ., Wang, YJ. et al. Transcriptome analysis of Actinoplanes utahensis reveals molecular signature of saccharide impact on acarbose biosynthesis. 3 Biotech 10, 473 (2020). https://doi.org/10.1007/s13205-020-02466-0
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DOI: https://doi.org/10.1007/s13205-020-02466-0