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A Fluorescence Study of the Interaction of Anticancer Drug Molecule Doxorubicin Hydrochloride in Pluronic P123 and F127 Micelles

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Abstract

Drug delivery systems for the sustained and target delivery of doxorubicin to tumor cells are a topic of interest due to the efficacy of the doxorubicin in cancer treatment. The use of polymers such as Pluronic is being studied widely for the formulation of doxorubicin hydrochloride. However, the basic understanding of the physicochemical properties of pluronic micelles in presence of doxorubicin hydrochloride is a very essential topic of study. Doxorubicin hydrochloride is fluorescent; this helped us to study its sensitivity towards the Pluronic microenvironment using the fluorescence technique. In this work, the interaction and place of location of doxorubicin hydrochloride in Pluronic F127 and P123 micelles has been studied extensively using steady-state fluorescence intensity, dynamic fluorescence lifetime, quenching studies, dynamic light scattering, and zeta potential measurements, at different Pluronic concentrations. Using a fluorescence quenching experiment, doxorubicin hydrochloride was found to reside near the hydrophilic PEO corona region of the Pluronic micelles. For both the Pluronic, in the concentration range of study, the micellar size was found to be below 30 nm; this may have a greater advantage for various applications.

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Acknowledgments

The authors thank Prof Vaidya Jayathirtha Rao (IICT Hyderabad, India) and Prof Ashok Kumar Mishra (IIT Madras, India) for their valuable discussion and help in collecting fluorescence lifetime data. Dr. M. Mohapatra and Mr. S. K. Behera acknowledge the DST- Science and Engineering Research Board (SERB) (Grant number: ECR/2016/000692), Government of India, and TEQIP- III, VSSUT Burla for financial support.

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  1. Monalisa Mohapatra

    Present address: School of Chemistry, Gangadhar Meher University, Sambalpur, Odisha, 768004, India

Authors and Affiliations

  1. Department of Chemistry, VSS University of Technology, Burla, Odisha, 768018, India

    Sagar Kumar Behera & Monalisa Mohapatra

  2. Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500007, India

    Maneesha Esther Mohanty

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Behera, S.K., Mohanty, M.E. & Mohapatra, M. A Fluorescence Study of the Interaction of Anticancer Drug Molecule Doxorubicin Hydrochloride in Pluronic P123 and F127 Micelles. J Fluoresc 31, 17–27 (2021). https://doi.org/10.1007/s10895-020-02630-y

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