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Radiochemical evidence validates the involvement of root released organic acid and phytosiderphore in regulating the uptake of phosphorus and certain metal micronutrients in wheat under phosphorus and iron deficiency

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Abstract

P availability determined the root uptake and root–shoot translocation of micronutrients. Total root 14C exudation was significantly higher under P−Fe− across wheat cultivars. Although malate than citrate was the predominant constituent of the root exudates in wheat, their release was higher under P than P+ condition, irrespective of the Fe availability.A higher release of phytosiderophores was measured under P−Fe− than P+Fe+ for bread than durum wheat. The study highlights the role of root exudates in influencing the mobilization, uptake and translocation of not only P and Fe but also Mn and to some extent Cu, particularly under the nutrient-starved (P−Fe−) condition of growth.

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Abbreviations

OA:

Organic acid

PS:

Phytosiderophore

DAT:

Days after transfer

PUE:

Phosphorus use efficiency

TI:

Translocation index

P:

Phosphorus

Mn:

Manganese

Cu:

Copper

Zn:

Zinc

fw:

Fresh weight

PVDF:

Polyvinylidene fluoride

DEAE-cellulose:

Diethylaminoethyl cellulose

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Acknowledgements

Raktim Mitra thanks ICAR for financial support during the Masters program at IARI, New Delhi.

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RM executed the experiments, collected and analysed the results; SBS helped in HPLC analysis of organic acids; BS conceptualized, facilitated and supervised the experiments and wrote the paper.

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Correspondence to Bhupinder Singh.

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Mitra, R., Singh, S.B. & Singh, B. Radiochemical evidence validates the involvement of root released organic acid and phytosiderphore in regulating the uptake of phosphorus and certain metal micronutrients in wheat under phosphorus and iron deficiency. J Radioanal Nucl Chem 326, 893–910 (2020). https://doi.org/10.1007/s10967-020-07383-3

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