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Developing a versatile electrochemical platform with optimized electrode configuration through screen-printing technology toward glucose detection

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Abstract

Rapid on-site detection of glucose has been attracting considerable attention nowadays. Screen-printed electrodes (SPEs) were assessed as ideal detection platforms due to their advantages such as, disposability, portability, ease of miniaturization, and mass production. The topology and shape of electrodes have a crucial impact on their electrical conductivity and electrochemical properties. In this study, a versatile electrochemical platform with optimized three-electrode configuration was developed through screen-printing technology. Three types of SPEs were prepared, and their electrocatalytic properties toward glucose detection were investigated. Based on this platform, both enzyme-based (denoted as GOD/rGO) and non-enzymatic (denoted as Co@MoS2/rGO) bioactive compounds were deposited on the working electrode of the circular SPE through simply drop-casting, respectively. Their morphology was characterized through scanning electron microscopy (SEM). Cycle sweep voltammetry was used to study the electrocatalytic activity of these biosensors. The circular SPE exhibited satisfying electrochemical redox activity and much higher sensitivity towards glucose detection, which rendered it a promising candidate for point-of-care detection.

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References

  • T.T. Baby, S.S.J. Aravind, T. Arockiadoss, et al., Metal decorated graphene nanosheets as immobilization matrix for amperometric glucose biosensor [J]. Sensors Actuators B Chem. 145(1), 71–77 (2010)

    Article  Google Scholar 

  • Cai J, Huang J, Ge M, et al. Immobilization of Pt Nanoparticles via Rapid and Reusable Electropolymerization of Dopamine on TiO2 Nanotube Arrays for Reversible SERS Substrates and Nonenzymatic Glucose Sensors [J]. Small, 2017, 13(19)

  • C. Chen, Q. Xie, D. Yang, et al., Recent advances in electrochemical glucose biosensors: A review [J]. RSC Adv. 3(14), 4473–4491 (2013)

    Article  Google Scholar 

  • Y. Dilmac, M. Guler, Fabrication of non-enzymatic glucose sensor dependent upon au nanoparticles deposited on carboxylated graphene oxide [J]. J. Electroanal. Chem. 864, 114091 (2020)

    Article  Google Scholar 

  • A.L. Galant, R.C. Kaufman, J.D. Wilson, Glucose: Detection and analysis [J]. Food Chem. 188, 149–160 (2015)

    Article  Google Scholar 

  • L. Ge, J. Yan, X. Song, et al., Three-dimensional paper-based electrochemiluminescence immunodevice for multiplexed measurement of biomarkers and point-of-care testing [J]. Biomaterials 33(4), 1024–1031 (2012)

    Article  Google Scholar 

  • A.L. Ghindilis, P. Atanasov, E. Wilkins, Enzyme-catalyzed direct electron transfer: Fundamentals and analytical applications [J]. Electroanalysis 9(9), 661–674 (1997)

    Article  Google Scholar 

  • J.P. Hart, S.A. Wring, Recent developments in the design and application of screen-printed electrochemical sensors for biomedical, environmental and industrial analyses [J]. TrAC Trends Anal. Chem. 16(2), 89–103 (1997)

    Article  Google Scholar 

  • T.K. Huang, K.W. Lin, S.P. Tung, et al., Glucose sensing by electrochemically grown copper nanobelt electrode [J]. J. Electroanal. Chem. 636(1–2), 123–127 (2009)

    Article  Google Scholar 

  • Laboratory Techniques in Electroanalytical Chemistry, revised and expanded [M]. CRC press, 2018

  • Lei Y, Zhao W, Zhang Y, et al. A MXene-Based Wearable Biosensor System for High-Performance In Vitro Perspiration Analysis [J]. Small, 2019, 15(19)

  • X. Li, L. Zhang, X. Zang, et al., Photo-promoted platinum nanoparticles decorated MoS2@ graphene woven fabric catalyst for efficient hydrogen generation [J]. ACS Appl. Mater. Interfaces 8(17), 10866–10873 (2016)

    Article  Google Scholar 

  • X. Li, K. Ren, M. Zhang, et al., Cobalt functionalized MoS2/carbon nanotubes scaffold for enzyme-free glucose detection with extremely low detection limit [J]. Sensors and Actuators B-chemical, 122–128 (2019)

  • X. Li, M. Zhang, Y. Hu, et al., Screen-printed electrochemical biosensor based on a ternary co@MoS2/rGO functionalized electrode for high-performance non-enzymatic glucose sensing [J]. Biomed. Microdevices 22(1), 17 (2020)

    Article  Google Scholar 

  • Liang T, Zou L, Guo X, et al. Rising Mesopores to Realize Direct Electrochemistry of Glucose Oxidase toward Highly Sensitive Detection of Glucose [J]. Adv. Funct. Mater., 2019, 29(44)

  • M. Libansky, J. Zima, J. Barek, et al., Basic electrochemical properties of sputtered gold film electrodes [J]. Electrochim. Acta 251, 452–460 (2017)

    Article  Google Scholar 

  • W.Q. Lim, Z. Gao, Metal oxide nanoparticles in electroanalysis [J]. Electroanalysis 27(9), 2074–2090 (2015)

    Article  Google Scholar 

  • J. Liu, C. Guo, C.M. Li, et al., Carbon-decorated ZnO nanowire array: A novel platform for direct electrochemistry of enzymes and biosensing applications [J]. Electrochem. Commun. 11(1), 202–205 (2009)

    Article  Google Scholar 

  • Y. Liu, Y. Liu, H. Feng, et al., Layer-by-layer assembly of chemical reduced graphene and carbon nanotubes for sensitive electrochemical immunoassay [J]. Biosens. Bioelectron. 35(1), 63–68 (2012)

    Article  Google Scholar 

  • X. Liu, W. Yang, L. Chen, et al., Synthesis of copper nanorods for non-enzymatic amperometric sensing of glucose [J]. Microchim. Acta 183(8), 2369–2375 (2016)

    Article  Google Scholar 

  • D. Maity, C.R. Minitha, R.T. Kumar, et al., Glucose oxidase immobilized amine terminated multiwall carbon nanotubes/reduced graphene oxide/polyaniline/gold nanoparticles modified screen-printed carbon electrode for highly sensitive amperometric glucose detection [J]. Mater. Sci. Eng. C 105, 110075 (2019)

    Article  Google Scholar 

  • S. Mansouri, J.S. Schultz, A miniature optical glucose sensor based on affinity binding [J]. Bio/Technology 2(10), 885–890 (1984)

    Google Scholar 

  • D. Merki, H. Vrubel, L. Rovelli, et al., Fe, co, and Ni ions promote the catalytic activity of amorphous molybdenum sulfide films for hydrogen evolution [J]. Chem. Sci. 3(8), 2515–2525 (2012)

    Article  Google Scholar 

  • Y. Miwa, M. Nishizawa, T. Matsue, et al., A conductometric glucose sensor based on a twin-microband electrode coated with a polyaniline thin film [J]. Bull. Chem. Soc. Jpn. 67(10), 2864–2866 (1994)

    Article  Google Scholar 

  • J.W. Mo, B. Ogorevc, Simultaneous measurement of dopamine and ascorbate at their physiological levels using voltammetric microprobe based on overoxidized poly (1, 2-phenylenediamine)-coated carbon fiber [J]. Anal. Chem. 73(6), 1196–1202 (2001)

    Article  Google Scholar 

  • M. Morikawa, N. Kimizuka, M. Yoshihara, et al., New colorimetric detection of glucose by means of electron-accepting indicators: Ligand substitution of [Fe (acac)3-n (phen)n]n+ complexes triggered by electron transfer from glucose oxidase [J]. Chem Eur J 8(24), 5580–5584 (2002)

    Article  Google Scholar 

  • X. Niu, M. Lan, H. Zhao, et al., Electrochemical stripping analysis of trace heavy metals using screen-printed electrodes [J]. Anal. Lett. 46(16), 2479–2502 (2013)

    Article  Google Scholar 

  • S. Phetsang, J. Jakmunee, P. Mungkornasawakul, et al., Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode [J]. Bioelectrochemistry 127, 125–135 (2019)

    Article  Google Scholar 

  • J.C. Pickup, F. Hussain, N.D. Evans, et al., Fluorescence-based glucose sensors [J]. Biosens. Bioelectron. 20(12), 2555–2565 (2005)

    Article  Google Scholar 

  • Z. Rafiee, A. Mosahebfard, M.H. Sheikhi, High-performance ZnO nanowires-based glucose biosensor modified by graphene nanoplates [J]. Mater. Sci. Semicond. Process. 115, 105116 (2020)

    Article  Google Scholar 

  • H.S.S. Ramakrishna Matte, A. Gomathi, A.K. Manna, et al., MoS2 and WS2 analogues of graphene [J]. Angew. Chem. Int. Ed. 49(24), 4059–4062 (2010)

    Article  Google Scholar 

  • M. Saraf, K. Natarajan, S.M. Mobin, et al., Non-enzymatic amperometric sensing of glucose by employing sucrose templated microspheres of copper oxide (CuO)[J]. Dalton Trans. 45(13), 5833–5840 (2016)

    Article  Google Scholar 

  • Selvarani, K., Sheela Berchmans. Biosensing of Cholesterol and Glucose Facilitated by Cationic Polymer Overlayers on Ni (OH)2/NiOOH at Physiological PH [J]. J. Electrochem. Soc., 2017, 164(9)

  • X. Shi, H. Gong, Y. Li, et al., Graphene-based magnetic plasmonic nanocomposite for dual bioimaging and photothermal therapy [J]. Biomaterials 34(20), 4786–4793 (2013)

    Article  Google Scholar 

  • P.R. Solanki, A. Kaushik, V.V. Agrawal, et al., Nanostructured metal oxide-based biosensors [J]. NPG Asia Materials 3(1), 17–24 (2011)

    Article  Google Scholar 

  • Sridara T, Upan J, Saianand G, et al. Non-Enzymatic Amperometric Glucose Sensor Based on Carbon Nanodots and Copper Oxide Nanocomposites Electrode [J]. Sensors, 2020, 20(3)

  • P. Sutter, How silicon leaves the scene [J]. Nat. Mater. 8(3), 171–172 (2009)

    Article  Google Scholar 

  • H.Y. Tseng, V. Adamik, J. Parsons, et al., Development of an electrochemical biosensor array for quantitative polymerase chain reaction utilizing three-metal printed circuit board technology [J]. Sensors Actuators B Chem. 204, 459–466 (2014)

    Article  Google Scholar 

  • Urhan B K, Demir U, Ozer T O, et al. Electrochemical fabrication of Ni nanoparticles-decorated electrochemically reduced graphene oxide composite electrode for non-enzymatic glucose detection [J]. Thin Solid Films, 2020

  • J. Wang, Electrochemical glucose biosensors [J]. Chem. Rev. 108(2), 814–825 (2008)

    Article  Google Scholar 

  • Y. Wang, H. Xu, J. Zhang, et al., Electrochemical sensors for clinic analysis [J]. Sensors 8(4), 2043–2081 (2008)

    Article  Google Scholar 

  • G. Wang, X. He, L. Wang, et al., Non-enzymatic electrochemical sensing of glucose [J]. Microchim. Acta 180(3–4), 161–186 (2013)

    Article  Google Scholar 

  • A.P. Washe, P. Lozano-Sánchez, D. Bejarano-Nosas, et al., Facile and versatile approaches to enhancing electrochemical performance of screen printed electrodes [J]. Electrochim. Acta 91, 166–172 (2013)

    Article  Google Scholar 

  • M.C. Weston, E.C. Anderson, P.U. Arumugam, et al., Redox magnetohydrodynamic enhancement of stripping voltammetry: Toward portable analysis using disposable electrodes, permanent magnets, and small volumes [J]. Analyst 131(12), 1322–1331 (2006)

    Article  Google Scholar 

  • L. Zhang, D.W. Li, W. Song, et al., High sensitive on-site cadmium sensor based on AuNPs amalgam modified screen-printed carbon electrodes [J]. IEEE Sensors J. 10(10), 1583–1588 (2010a)

    Article  Google Scholar 

  • Q. Zhang, Y. Qiao, F. Hao, et al., Fabrication of a biocompatible and conductive platform based on a single-stranded DNA/Graphene Nanocomposite for direct electrochemistry and Electrocatalysis [J]. Chemistry: A European Journal 16(27), 8133–8139 (2010b)

    Article  Google Scholar 

  • M. Zhou, Y. Zhai, S. Dong, Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide [J]. Anal. Chem. 81(14), 5603–5613 (2009)

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Key Scientific Instrument and Equipment Development Project (Grant No.: 51627808), National Natural Science Foundation of China (Grant No. 51605088), Natural Science Foundation of Jiangsu Province (Grant No. BK20170667) and Shuangchuang Talent Project of Jiangsu Province (Grant No. 1102000208).

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Correspondence to Tao Hu or Zhonghua Ni.

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Li, X., Zhang, M., Hu, Y. et al. Developing a versatile electrochemical platform with optimized electrode configuration through screen-printing technology toward glucose detection. Biomed Microdevices 22, 74 (2020). https://doi.org/10.1007/s10544-020-00527-y

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