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Primary mapping of QTL for growth-related traits in grass carp (Ctenopharyngodon idellus)

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Abstract

Growth-related traits are economically important in aquaculture, and their variation is linked to quantitative loci (QTLs). For exploring genetic basis of genetic breeding and improvement of grass carp (Ctenopharyngodon idellus), QTL mapping for growth-related traits was performed with an F2 family of grass carp from the Yangtze River system, including 360 offspring. In this study, we mapped QTLs for body weight, body length, body height, and body width using 99 previously published microsatellite loci. A total of 15 growth-related QTLs were found in 7 linkage groups (LG1, LG2, LG14, LG15, LG16, LG18, and LG21). Four QTLs were related to body weight (qBWH1, qBWH14, qBWH15, and qBWH16), and explained 3.1 to 7.7% of the phenotypic variance. Six QTLs were related to body length (qBL1, qBL2, qBL14, qBL15, qBL16, and qBL18), and these QTLs explained 2.8 to 8.9% of the phenotypic variance. Three QTLs affecting body height (qBH14, qBH15, and qBH16) explained 3.2 to 9.2% of the phenotypic variance. Two QTLs affecting body width (qBW15 and qBW21) accounted for 9.2% and 3.3% of the phenotypic variance, respectively. In order to fine map the QTL affecting body weight on LG1 (qBWH1), 14 additional novel microsatellites were included, and the qBWH1 was relocated with a narrower flanking marker interval of 7.5 cM. This is the first study of QTL mapping in grass carp and provides new insights for the application of molecular marker–assisted breeding in this species.

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Acknowledgments

We thank Mr. Ye Baoqing and Santosh for English editing.

Funding

This work was supported by the Project of Shanghai Engineering and Technology Center for Promoting Ability (19DZ2284300) and China Agriculture Research System (CARS-45-03).

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Authors and Affiliations

  1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China

    Chengchen Yu, Xiaoyan Xu, Jiale Li & Yubang Shen

  2. Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China

    Chengchen Yu, Xiaoyan Xu, Jiale Li & Yubang Shen

  3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China

    Chengchen Yu, Xiaoyan Xu, Jiale Li & Yubang Shen

  4. College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Yubang Shen

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  1. Chengchen Yu
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Correspondence to Yubang Shen.

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Figure S1

The frequency histogram of the phenotypic data including body weight (BWH), body length (BL), body height (BH), and body width (BW). (PNG 3468 kb)

High resolution image (TIF 1608 kb)

Figure S2

QTL mapping for growth-related traits. (a), QTL for body weight on LG1, LG14, LG15, LG16; (b), QTL for body length on LG1, LG2, LG14, LG15, LG16, LG18; (c), QTL for body height on LG16, LG18; (d), QTL for body width on LG15, LG21. The Y-axis presents LOD scores and the X-axis presents genetic distances. The red lines were drawn by plotting the LOD scores at each marker along the linkage group. (PNG 6377 kb)

High resolution image (TIF 1148 kb)

Figure S3

QTL mapping for weight-related trait on LG1. The Y-axis presents LOD scores and the X-axis presents genetic distances. The red line was drawn by plotting the LOD scores at each marker along the linkage group. (PNG 1342 kb)

High resolution image (TIF 74 kb)

ESM 4

Details of the linkage map. The asterisk after microsatellites indicates the marker showing segregation distortion. (XLSX 28 kb)

Table S1

Characteristics of the 14 additional novel microsatellites from grass carp (Ctenopharyngodon idellus). (DOC 53 kb)

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Yu, C., Xu, X., Li, J. et al. Primary mapping of QTL for growth-related traits in grass carp (Ctenopharyngodon idellus). Aquacult Int 28, 2275–2285 (2020). https://doi.org/10.1007/s10499-020-00594-1

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  • DOI: https://doi.org/10.1007/s10499-020-00594-1

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