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Identification and expression analysis of the Hevea brasiliensis phosphate transporter 1 gene family

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Abstract

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Twelve HbPHT1s were identified in Hevea and most of them were induced by phosphate starvation. HbPHT1;5 complemented the defective yeast mutant strain EY917 and HbPHT1;7 only partly complemented it.

Abstract

Phosphorus is an important element of the latex of rubber tree, and its content affects the quality and yield of latex. However, with the outflow of latex, a large amount of phosphorus is lost. The phosphate transporter 1 (PHT1) family proteins regulate the phosphorus acquisition and translocation in the plants, but their expression and roles in Hevea brasiliensis remain unclear. In this work, we identified twelve HbPHT1s (HbPHT1;1 to HbPHT1;12). Evolutionary analyses suggested two duplication events: HbPHT1;5/HbPHT1;6 and HbPHT1;7/HbPHT1;8. In the promoter region of some HbPHT1s, there existed P1BS, W-box, MYB, and ethylene response cis-elements. All the HbPHT1s, except for HbPHT1;4, were expressed in the roots, and most of them were also expressed in the stems and leaves. HbPHT1;7 had the highest expression level in the roots, stems, and leaves. HbPHT1;5 and HbPHT1;6 had a relatively higher expression levels than other genes in latex. HbPHT1;5, HbPHT1;7, and HbPHT1;11 were induced by ethylene treatment. Most HbPHT1s were induced under phosphate starvation conditions in the seedling leaves, and the expression of HbPHT1;9 was 20-fold higher than the control. HbPHT1;5 complemented the deficiency of the yeast mutant strain EY917 in phosphate transportation under low- and high-phosphate conditions, and HbPHT1;7 restored the deficiency when 10 mM phosphate was supplied. These results suggested that the increased expression of PHT1s may improve the phosphate uptake, transport, and utilization in Hevea. It also provides the basis to sudy the phosphorus metabolism network and mine the gene resources for the genetic breeding of Hevea.

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Abbreviations

PHT1:

Phosphate transporter 1

P:

Phosphorus

Pi:

Phosphate

MEME:

Multiple Em for Motif Elicitation

GSDS:

Gene Structure Display Server

P1BS:

PHR1 specific binding sequence

ACC:

1-Amino cyclopropane-1-carboxylic acid

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Acknowledgements

This research was supported by the Hainan Provincial Natural Science Foundation of China (No. 319MS088), the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630022017008, 1630052017007), the Hainan Provincial Science and Technology Project of China (No. ZDYF2018240).

Funding

This research was supported by the Hainan Provincial Natural Science Foundation of China (No. 319MS088), the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630022017008, 1630052017007), the Hainan Provincial Science and Technology Project of China (No. ZDYF2018240).

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  1. Yong Sun and Le Gao contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China

    Yong Sun, Le Gao, Bingsun Wu, Min Wu, Guihua Wang, Wenguan Wu, Jiashao Wei & Peng He

  2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China

    Dan Wang & Zheng Tong

  3. College of Tropical Crops, Hainan University, Haikou, China

    Renjun Feng

Authors
  1. Yong Sun
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  2. Le Gao
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  3. Dan Wang
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  4. Bingsun Wu
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  10. Renjun Feng
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  11. Peng He
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Contributions

Peng He and Yong Sun conceived and designed the experiments. Wenguan Wu, Bingsun Wu, and Guihua Wang prepared the materials. Yong Sun, Jiashao Wei, and Renjun Feng participated in the experiments. Dan Wang, Zheng Tong, Yong Sun, and Min Wu performed data analysis. The first draft of the manuscript was written by Yong Sun and Le Gao, all of the authors commented on previous versions of the manuscript and read and approved the final manuscript.

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Correspondence to Renjun Feng or Peng He.

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The authors declare that they have no competing interests. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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Communicated by Pierre Emmanuel Courty.

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Sun, Y., Gao, L., Wang, D. et al. Identification and expression analysis of the Hevea brasiliensis phosphate transporter 1 gene family. Trees 35, 407–419 (2021). https://doi.org/10.1007/s00468-020-02042-2

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