Abstract
The most important potato viruses in Uganda are Potato virus Y (PVY), Potato leafroll virus (PLRV), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) and Potato virus M (PVM). Utilization of R genes in breeding for resistance to viruses has not been explored in Uganda due to limited information on the prevalence of R genes in the available genotypes. This study aimed at identifying potato genotypes with R genes for resistance to potato viruses important in Uganda. The study screened 71 potato accessions from the National Potato Breeding Programme at Kachwekano Zonal Agricultural Research and Development Institute for the presence of resistance genes to viruses using diagnostic molecular markers. The results indicated that 21 out of 71 genotypes had resistance markers, of which nine genotypes, NKRN59.58, Derby, Markies, Sifra, 395017.229, Nakpot 5, 20108.5, Royal and 393220.54, had Ryadg gene for PVY resistance and two genotypes, Kimuri and 319919.3, had Rysto gene for resistance to PVY and PVA. Nine genotypes, 395011.2, Markies, Nakpot 5, 20108.5, Sifra, 20157.6, Royal, 2015.8 and Ambition, had the Nbtbr gene for resistance to PVX. In addition, 14 genotypes, 395011.2, Markies, Nakpot 5, Sarpouna, 393220.54, 391046.14, Sarpomira, 395077.12, Sifra, 20157.6, Royal, Ambition, Kimuri and Caruso, had the Nsadg gene conferring resistance to PVS. Four genotypes, Markies, Sifra, Nakpot 5 and Royal, had the Ryadg, Nbtbr and Nsadg genes for combined resistance to PVY, PVX and PVS. The resistant genotypes could be used as parents to introgress resistance genes into susceptible cultivars.
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References
Antanaviciute L, Harrison N, Battey NH, Harrison RJ (2015) An inexpensive and rapid genomic DNA extraction protocol for Rosaceous species. J Hortic Sci Biotechnol 90(4):427–432. https://doi.org/10.1080/14620316.2015.11513205
Bendahmane A, Kanyuka K, Baulcombe DC (1999) The Rx gene from potato controls separate virus resistance and cell death responses. Plant Cell 11(5):781–791. https://doi.org/10.1105/tpc.11.5.781
Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbet assay for the detection of plant viruses. J Gen Virol 34:475–483. https://doi.org/10.1099/0022-1317-34-3-475
Cockerham G (1970) Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348. https://doi.org/10.1038/hdy.1970.35
Dupuis B, Bragard C, Carnegie S, Kerr J, Glais L, Singh M Lacomme C (2017) Potato Virus Y: control, management and seed certification Programmes. In Potato Virus Y: biodiversity, pathogenicity, epidemiology and management (pp. 177-206). Springer, Cham. https://doi.org/10.1007/978-3-319-58860-5_7
Dziewonska MA, Ostrowska K (1978) Resistance to potato virus M in certain potato species. Potato Res 21:129–131
Gebhardt C, Bellin D, Henselewski H, Lehmann W, Schwarzfischer J, Valkonen JPT (2006) Marker-assisted combination of major genes for pathogen resistance in potato. Theor Appl Genet 112(8):1458–1464. https://doi.org/10.1007/s00122-006-0248-8
Gopal J, Khurana SM (2006) Consideration for successful breeding. In: Handbook of potato production, improvement, and postharvest management. Food Product Press, New York, pp 77–108. https://doi.org/10.1201/9780429246623
Heldák J, Bežo M, ŠtefúnoVá V, GAllikoVá A (2007) Selection of DNA markers for detection of extreme resistance to Potato Virus Y in tetraploid potato. Czech J Genet Plant Breed 43(4):125–134. https://doi.org/10.17221/2074-CJGPB
Kasai K, Morikawa Y, Sorri VA, Valkonen JPT, Gebhardt C, Watanabe KN (2000) Development of SCAR markers to the PVY resistance gene Ryadg based on a common feature of plant disease resistance genes. Genome Res 43:1–8. https://doi.org/10.1139/g99-092
KAZARDI (2016) Virus cleaning of farmer preferred potato varieties in Uganda report submitted to International Fertilizer Development Centre (IFDC) (unpublished)
Kohl TO, Ascoli CA (2017) Indirect immunometric ELISA. Cold Spring Harb. Protoc. https://doi.org/10.1101/pdb.top093690
Lee PY, Costumbrado J, HSu CY, Kim YH (2012) Agarose gel electrophoresis for the separation of DNA fragments. Jove-J Vis Exp 62. https://doi.org/10.3791/3923
Lopez-Pardo R, Barandalla L, Ritter E, Ruiz de Galarreta JI (2013) Validation of molecular markers for pathogen resistance in potato. Plant Breed 132(3):246–251. https://doi.org/10.1111/pbr.12062
Mackenzie TD, Nie X, Singh M (2016) Crop management practices and reduction of on-farm spread of Potato virus Y: a 5-year study in commercial potato fields in New Brunswick, Canada. Am Potato J 93(6):552–563. https://doi.org/10.1007/s12230-016-9534-4
Marano MR, Malcuit I, De Jong W, Baulcombe DC (2002) High-resolution genetic map of Nb, a gene that confers hypersensitivity to Potato virus X in Solanum tuberosum. Theor Appl Genet 105:192–200. https://doi.org/10.1007/s00122-002-0962-9
Marczewski W, Flis B, Syller J, Schäfer-Preg R, Gebhardt C (2001) A major quantitative trait locus for resistance to Potato leafroll virus is located in a resistance hotspot on potato chromosome XI and is tightly linked to N-gene-like markers. Mol Plant-Microbe Interact 14:1420–1425. https://doi.org/10.1094/MPMI.2001.14.12.1420
Marczewski W, Hennig J, Gebhardt C (2002) The Potato virus S resistance gene Ns maps to potato chromosome VIII. Theor Appl Genet 105(4):564–567. https://doi.org/10.1007/s00122-002-0976-3
Marczewski W, Strzelczyk-Żyta D, Hennig J, Witek K, Gebhardt C (2006) Potato chromosomes IX and XI carry genes for resistance to potato virus M. Theor Appl Genet 112(7):1232–1238. https://doi.org/10.1007/s00122-006-0224-3
Priegnitz U, Lommen WJM, Van der Vlugt RA, Struik PC (2019) Impact of positive selection on incidence of different viruses during multiple generations of potato seed tubers in Uganda. Potato Res 62(1):1–30. https://doi.org/10.1007/s11540-018-9394-z
Ramakrishnan AP, Ritland CE, Sevillano RHB, Riseman A (2015) Review of potato molecular markers to enhance trait selection. Am Potato J 92(4):455–472. https://doi.org/10.1007/s12230-015-9455-7
Rao GP, Reddy MG (2020) Overview of yield losses due to plant viruses. In Applied plant virology (pp. 531-562). Academic Press. https://doi.org/10.1016/B978-0-12-818654-1.00038-4
Rosario HM, Vidalon LJ, Montenegro JD, Riccio C, Guzman F, Bartolini I, Ghislain M (2018) Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y. Theor Appl Genet 131(9):1925–1938. https://doi.org/10.1007/s00122-018-3123-5
Szajko K, Chrzanowska M, Witek K, Strzelczyk-Żyta D, Zagórska H, Gebhardt C, Marczewski W (2008) The novel gene Ny-1 on potato chromosome IX confers hypersensitive resistance to Potato virus Y and is an alternative to Ry genes in potato breeding for PVY resistance. Theor Appl Genet 116(2):297–303. https://doi.org/10.1007/s00122-007-0667-1
Szajko K, Strzelczyk-Żyta D, Marczewski W (2014) Ny-1 and Ny-2 genes conferring hypersensitive response to Potato virus Y (PVY) in cultivated potatoes: mapping and marker-assisted selection validation for PVY resistance in potato breeding. Mol Breeding 34(1):267–271. https://doi.org/10.1007/s11032-014-0024-4
Tiwari JK, Singh BP (2012) Marker-assisted selection for virus resistance in potato: options and challenges. Potato J 39(2):101–117
Van Berloo R, Hutten RCB, Van Eck HJ, Visser RGF (2007) An online potato pedigree database resource. Potato Res 50(1):45–57. https://doi.org/10.1007/s11540-007-9028-3
Velásquez AC, Mihovilovich E, Bonierbale M (2007) Genetic characterization and mapping of major gene resistance to Potato leafroll virus in Solanum tuberosum ssp. andigena. Theor Appl Genet 114:1051–1058. https://doi.org/10.1007/s00122-006-0498-5
Whitworth JL, Novy RG, Hall DG, Crosslin JM, Brown CR (2009) Characterization of broad spectrum Potato Virus Y resistance in a Solanum tuberosum ssp. andigena-derived population and select breeding clones using molecular markers, grafting, and field inoculations. Am. Potato J 86(4):286–296. https://doi.org/10.1007/s12230-009-9082-2
Zhang W, Zhang Z, Fan G, Gao Y, Wen J, Bai Y, Meng X (2017) Development and application of a universal and simplified multiplex RT-PCR assay to detect five potato viruses. J Gen Plant Pathol 83(1):33–45
Acknowledgements
The study was conducted as part of a PhD thesis with funding from National Agricultural Research organization. The authors acknowledge the support of Mr. Allan Male and Mr. Alli Kajubi for technical support in molecular marker analysis.
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Byarugaba, A.A., Mukasa, S.B., Barekye, A. et al. Prevalence of R Genes for Resistance to Potato Viruses in Uganda Germplasm. Potato Res. 64, 267–276 (2021). https://doi.org/10.1007/s11540-020-09477-7
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DOI: https://doi.org/10.1007/s11540-020-09477-7