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A CCR4-associated factor 1, OsCAF1B, confers tolerance of low-temperature stress to rice seedlings

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Rice is an important crop in the world. However, little is known about rice mRNA deadenylation, which is an important regulation step of gene expression at the post-transcriptional level. The CCR4–NOT1 complex contains two key components, CCR4 and CAF1, which are the main cytoplasmic deadenylases in eukaryotic cells. Expression of OsCAF1B was tightly coupled with low-temperature exposure. In the present study, we investigated the function of OsCAF1B in rice by characterizing the molecular and physiological responses to cold stress in OsCAF1B overexpression lines and dominant-negative mutant lines. Our results demonstrate that OsCAF1B plays an important role in growth and development of rice seedlings at low temperatures.

Abstract

Rice is a tropical and subtropical crop that is sensitive to low temperature, and activates a complex gene regulatory network in response to cold stress. Poly(A) tail shortening, also termed deadenylation, is the rate-limiting step of mRNA degradation in eukaryotic cells. CCR4-associated factor 1 (CAF1) proteins are important enzymes for catalysis of mRNA deadenylation in eukaryotes. In the present study, the role of a rice cold-induced CAF1, OsCAF1B, in adaptation of rice plants to low-temperature stress was investigated. Expression of OsCAF1B was closely linked with low-temperature exposure. The increased survival percentage and reduced electrolyte leakage exhibited by OsCAF1B overexpression transgenic lines subjected to cold stress indicate that OsCAF1B plays a positive role in rice growth under low ambient temperature. The enhancement of cold tolerance by OsCAF1B in transgenic rice seedlings involved OsCAF1B deadenylase gene expression, and was associated with elevated expression of late-response cold-related transcription factor genes. In addition, the expression level of OsCAF1B was higher in a cold-tolerant japonica rice cultivar than in a cold-sensitive indica rice cultivar. The results reveal a hitherto undiscovered function of OsCAF1B deadenylase gene expression, which is required for adaptation to cold stress in rice.

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Acknowledgements

This work was supported by grants from the Ministry of Science and Technology of the Republic of China (no. 105-2321-B-008-001 and 106-2311-B-008-003-MY3) and also was financially supported (in part) by the Advanced Plant Biotechnology Center from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan.

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  1. Department of Life Sciences, National Central University, Jhongli City, Taoyuan County, 320, Taiwan, ROC

    Jhen-Cheng Fang, Yin-Chuan Tsai, Wei-Lun Chou, Hsin-Yi Liu, Chun-Chen Chang, Shaw-Jye Wu & Chung-An Lu

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  1. Jhen-Cheng Fang
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  2. Yin-Chuan Tsai
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  3. Wei-Lun Chou
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Contributions

JCF and CAL designed the research; JCF, YCT, WLC, HYL, and CCC performed the experiments; JCF, SJW, and CAL wrote the manuscript.

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Correspondence to Chung-An Lu.

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Supplementary file1 Supplemental Table S1. Primers used in the study. (DOCX 22 kb)

11103_2020_1079_MOESM2_ESM.pptx

Supplementary file2 Supplemental Fig. S1. Quantitative RT-PCR analysis of OsCAF1A, OsCAF1G, and OsCAF1H transcript levels in the OsCAF1B and OsCAF1Bm overexpression lines. Supplemental Fig. S2. Comparison of the vegetative morphology of the wild type, BOs, and BMs seedlings under cold stress. (PPTX 342 kb)

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Fang, JC., Tsai, YC., Chou, WL. et al. A CCR4-associated factor 1, OsCAF1B, confers tolerance of low-temperature stress to rice seedlings. Plant Mol Biol 105, 177–192 (2021). https://doi.org/10.1007/s11103-020-01079-8

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