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First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity

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Abstract

A severe vascular pomegranate wilt (Punica granatum L.) has recently been discovered and reported for the first time in Upper Egypt. Diseased samples were obtained from an infected farm in El-Badari city, Assiut Governorate, Egypt, and the pathogen was isolated on potato dextrose agar at 25 °C. Phenotypic features and DNA sequencing of the pathogen's internal transcribed spacer region showed that the fungus was Fusarium oxysporum. The pathogenicity study conducted with 2-year-old plants resulted in 80% infection. F. oxysporum re-isolated from all the inoculated pomegranate trees exhibited the same morphological characters as those observed among the isolates.

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Acknowledgments

This work was financially supported by the Academy of Scientific Research & Technology (ASRT), Egypt, Grant No. 6371 under the project Science Up. The authors greatly acknowledge the kind help and the keen assistance of all staff of the Assiut University Mycological Centre (AUMC).

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Correspondence to Sedky H. A. Hassan.

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Mohamed, R.A., Al-Bedak, O.A. & Hassan, S.H.A. First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity. J Plant Dis Prot 128, 311–316 (2021). https://doi.org/10.1007/s41348-020-00385-z

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