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Effect of light quality and tissue origin on phenolic compound accumulation and antioxidant activity in Camellia japonica calli

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Abstract

In Camellia japonica callus culture, the effects of light quality on the accumulation of phenolic compounds were examined. To this end, the calli were cultured under three monochrome [white (W), red (R), and blue (B)] and two mixed [red/blue (RB) and red/green/blue (RGB)] light sources. After 4 weeks of culture, fresh and dry callus weights were determined, and phenolic content was analyzed using high-performance liquid chromatography. We also examined the effects of RGB light on calli derived from four origins (leaf: CL; root: CR; petal: CP; and ovary: CO). Total phenolic and flavonoid content and DPPH radical scavenging activity were highest in calli exposed to RB and RGB. Catechin was detected only in calli exposed to mixed light (RB and RGB). Interestingly, red pigmentation development in CL and CR calli after 2 weeks of culture and organogenic calli in CR and CP samples was observed. Furthermore, expression of the phenylalanine ammonia lyase and chalcone synthase genes was found to be higher in the CR line than in other lines, and total phenolic and flavonoid content was also higher in the CR and CP lines. The findings of the present study revealed that the accumulation of phenolics and flavonoids in callus tissue varies depending on light quality, which stimulates cell division, and may thus affect the proliferation and organogenesis of C. japonica callus. We also established that the effects of light on red pigment expression and the accumulation of bioactive compounds are dependent on the tissue origin of callus material.

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Funding

This work was supported by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number 315013-4), and the authors were partially supported by BK 21 of Chungbuk National University.

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Authors and Affiliations

  1. Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Eun Bi Jang & So-Young Park

  2. Biodiversity Research Institute, Jeju Technopark, Shinyedong-ro 338, Seogwipo-si, Jeju, 63608, Republic of Korea

    Eun Bi Jang

  3. Institute for Global Health Innovations, Duy Tan University, Da Nang, 550000, Vietnam

    Thanh-Tam Ho

  4. Faculty of Pharmacy, Duy Tan University, Da Nang, 550000, Vietnam

    Thanh-Tam Ho

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  1. Eun Bi Jang
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  2. Thanh-Tam Ho
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Contributions

EB Jang contributed to the data acquisition and wrote the manuscript. TT Ho participated in the experiment and sample analysis. S-Y Park made substantial contributions to data interpretation, revising of the manuscript, the conception, and design of the study.

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Correspondence to So-Young Park.

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Editor: Yong Eui Choi

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Jang, E.B., Ho, TT. & Park, SY. Effect of light quality and tissue origin on phenolic compound accumulation and antioxidant activity in Camellia japonica calli. In Vitro Cell.Dev.Biol.-Plant 56, 567–577 (2020). https://doi.org/10.1007/s11627-020-10121-9

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