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The complete sequence of a papaya ringspot virus (PRSV) isolate from West Bengal, India infecting papaya and study of genetic variation

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Abstract

Papaya ringspot virus (PRSV) is one of the important pathogens responsible for damage of the papaya crop and causes significant yield loss of fruits and vegetable worldwide and hence a serious threat. In this study, PRSV-infected plant samples collected from different locations in North 24 Parganas districts of West Bengal, were tested with potyvirus specific ELISA and further confirmed with potyvirus group specific degenerate primers by RT PCR, TEM and sequence analysis. Our result indicates that the disease symptoms occurred in 80–100% of the plant in the field surveyed. The complete sequence of PRSV isolate WB was determined from RT-PCR products with specific primer pairs designed covering the whole genome and confirmed by next-generation sequencing. The whole genome of the PRSV was determined and submitted to GenBank as accession no. LC482263. The sequence of PRSV-WB (10,340 bp) was found to share upto 90% and 93%, identity with other PRSV isolates at the nucleotide and amino acid levels respectively. Based on phylogenetic analysis, PRSV- WB was found to be most closely related to PRSV-BD2 (MH397222) from papaya in Bangladesh (geographically close).

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Acknowledgements

Authors would like to thank Department of Science and Technology, New Delhi for SERB Project Grant No. SR/FT/LS-165/2012 and financial assistant from University Grants Commission (UGC), New Delhi to Miss Siriya Sultana, Senior Research Fellow (Maulana Azad National Fellowship) in the form of fellowship is greatly acknowledged. Authors also acknowledge the WB DST supported FIST and Boost program for the facilities to carry out the experiments.

Funding

The work of S.S. was supported by University Grants Commission (UGC), New Delhi as Senior Research Fellowship under Maulana Azad National Fellowship, and the work from A.R.S was funded by Department of Science and Technology, New Delhi for SERB Project Grant No. SR/FT/LS-165/2012.

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Authors and Affiliations

  1. Department of Botany, West Bengal State University, Malikapur, Berunanpukuria, 24 North Parganas, Barasat, Kolkata, W.B., 700126, India

    Siriya Sultana, Buddhadeb Roy & Ang Rinzing Sherpa

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  1. Siriya Sultana
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  2. Buddhadeb Roy
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  3. Ang Rinzing Sherpa
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Correspondence to Ang Rinzing Sherpa.

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This article does not contain any studies with human participants or animals performed by any of the authors

Electronic supplementary material

Supplementary Table 1

List of primers used for the generation of whole genome sequence of PRSV. (DOC 49 kb)

Supplementary Table 2

Summary of recombination analysis of the PRSV complete genome sequences with seven methods. (DOCX 19 kb)

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Sultana, S., Roy, B. & Sherpa, A.R. The complete sequence of a papaya ringspot virus (PRSV) isolate from West Bengal, India infecting papaya and study of genetic variation. Eur J Plant Pathol 159, 203–210 (2021). https://doi.org/10.1007/s10658-020-02124-4

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  • DOI: https://doi.org/10.1007/s10658-020-02124-4

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