Abstract
‘Peroba rosa’ (Aspidosperma polyneuron Muell. Arg.) is a forest species native to Brazil, which is threatened of extinction due to intense predatory exploitation. Knowing the physiological and sanitary aspects of the seeds or seedlings of native species is essential to determine the viability of the establishment of seedlings and natural regeneration. In tropical regions such as Brazil, the susceptibility of phytopathogens in native forest species is still poorly understood. In this work, we report for the first time Fusarium species associated with pathogenicity in Aspidosperma polyneuron. We identified a diverse group of crop pathogens Fusarium species - F. solani, F. oxysporum, F. verticillioides, F. equiseti, F. fujikuroi, F. pseudocircinatum and F. subglutinans from seeds, able to cause darkening of the leaf limb of inoculated seedlings. These results provide valuable information on the pathogenic association of Fusarium with an indigenous forest tree and possible risks these fungi might pose to the forest species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Americas Regional Workshop (Conservation & Sustainable Management of Trees, Costa Rica, November 1996). (1998). Aspidosperma polyneuron. The IUCN Red List of Threatened Species 1998: e.T32023A9674981. https://doi.org/10.2305/IUCN.UK.1998.RLTS.T32023A9674981.en. Accessed 27 October 2019.
Anderson, R. L. (1986). A new method for assessing contamination of slash and loblolly pine seeds by Fusarium moniliforme var. subglutinans. Plant Disease, 70(5), 452–453.
Andrade, L. A., Pereira, I. M., & Dornelas, G. V. (2002). Análise da vegetação arbóreo-arbustiva, espontânea, ocorrente em taludes íngremes no município de Areia – Estado da Paraíba. Revista Árvore, 26, 165–172.
Anjos, J. R. N., Charchar, M. J. d.’ A., Leite, R. G., & Silva, M. S. (2009). Levantamento e patogenicidade de fungos associados às sementes de mangaba (Harconia speciosa) no cerrado do Brasil Central. Revista Brasileira de Fruticultura, 31, 911–915.
Araújo, W. L., Lima, A. O. S., Azevedo, J. L., Marcon, J., Kublincky-Sobral, J., & Lacava, P. T. (2002). Manual: isolamento de microrganismos endofíticos. Piracicaba: ESALQ.
Benetti, S. C., Santos, A. F., Medeiros, A. C. S., & Jaccound Filho, D. S. (2009). Levantamento de fungos em sementes de cedro e avaliação da patogenicidade de Fusarium sp. e Pestalotia sp. Pesquisa Florestal Brasileira, 58, 79–83.
Bonfield, J., Beal K., Jordan, M., Chen, Y., Staden, R. (2006). The Staden package manual, Cambridge, UK.
Carmo, A. L. M., Mazarotto, E. J., Eckstein, B., & Santos, A. F. (2017). Associação de fungos com sementes de espécies florestais nativas. Summa Phytopathologica, 43, 246–247.
Carneiro, J. S. (1987). Testes de sanidade de sementes de essências florestais. In: Soave, J., Wetzel, M. M. V. S. Patologia de Sementes. Campinas: Fundação Cargill, 386-393.
Carvalho, W. L., & Muchovej, J. J. (1991). Fungos associados às sementes de essências florestais. Revista Árvore, 15, 173–178.
Carvalho, P. E. R. (2004). Peroba Rosa - Aspidosperma polyneuron. Colombo: Embrapa Florestas, (Circular Técnica, 96).
Correll, J. C., Gordon, T. R., McCain, A. H., Fox, J. W., Koehler, C. S., Wood, D. L., & Schultz, M. E. (1991). Pitch canker disease in California: Pathogenicity, distribution, and canker development on Monterey pine (Pinus radiata). Plant Disease, 75, 676–682.
Costa Júnior, J. C., Santos, A. F., Franciscon, L., Silva, C. N., & Tessmann, D. J. (2016). Qualidade sanitária e fisiológica, métodos de detecção de Fusarium spp. e tratamento de sementes de pupunheira. Ciência Florestal, 26, 1119–1131.
Dawidziuk, A., Koczyk, G., Popiel, D., Kaczmarek, J., & Buśko, M. (2014). Molecular diagnostics on the toxigenic potential of Fusarium spp. plant pathogens. Journal Applied Microbiology, 116, 1607–1620.
D’Mello, J. P. F., Placinta, C. M., & Macdonald, A. M. C. (1999). Fusarium mycotoxins: A review of global implications for animal health, welfare and productivity. Animal Feed Science and Technology, 80, 183–205.
Dorner, J. (2002). An introduction to using native plants in restoration projects (66p). Washington: Center for urban horticulture, University of Washington.
Fantinel, V. S., Oliveira, L. M., Casa, R. T., Schneider, P. F., Rocha, E. C., Vicente, D., & Pozzan, M. (2017). Detecção de fungos em sementes de Acca sellowiana (Berg) Burret. Floresta e Ambiente, 24, 1–11.
Fisher, M. C., Henk, D. A., Briggs, C. J., Brownstein, J. S., Madoff, L. C., McCraw, S. L., & Gurr, S. J. (2012). Emerging fungal threats to animal, plant and ecosystem health. Nature, 484, 186–194.
Geiser, D. M., Jimenez-Gasco, M. M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T. J., Zhang, N., Kuldau, G. A., & O’Donnell, K. (2004). FUSARIUM-ID v.1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology, 110, 473–479.
Gerrits van den Ende, A. H. G., & de Hoog, G. S. (1999). Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana. Studies in Mycology., 43, 151–162.
Grigoletti Junior, A., Auer, C. G., Maschio, L. M. A. (1996). Doenças em erva-mate (Ilex paraguariensis St. Hil.) na região sul do Brasil. Pesquisa Florestal Brasileira. 32/33, 43-51.
Herron, D. A., Wingfield, M. J., Wingfield, B. D., Rodas, C. A., Marincowitz, S., & Steenkamp, E. T. (2015). Novel taxa in the Fusarium fujikuroi species complex from Pinus spp. Studies in Mycology, 80, 131–150.
Lazarotto, M., Muniz, M. F. B., & Santos, A. F. (2010). Detection, transmission, pathogenicity and chemical treatment of fungi in Ceiba speciosa seeds. Summa Phytopathologica, 36, 134–139.
Leslie, J. F., & Summerell, B. A. (2006). The Fusarium laboratory manual. Ames: Blackwell Professional.
Maciel, C. G., Muniz, M. F. B., Santos, A. F., & Lazarotto, M. (2012). Detecção, transmissão e patogenicidade de fungos em sementes de angico-vermelho (Parapiptadenia rigida). Summa Phytopathologica, 38, 323–328.
Maciel, C. G., Muniz, M. F. B., Milanesi, P. M., Lazarotto, M., Blume, E., Reiniger, L. R. S., & Hamann, F. A. (2013). First report of Fusarium sambucinum associated on Pinus elliottii seeds in Brazil. Plant Disease, 97, 995.
Maciel, C. G., Walker, C., Santos, R. F., Muniz, M. F. B., & Brum, D. L. (2017). Fusarium oxysporum and F. verticillioides associated with damping-off in Pinus spp. Revista Ciência Agronômica, 48, 134–141.
Mazarotto, E. J., Pimentel, I. C., Abreu, D. C. A., & Santos, A. F. (2019). Association of Fusarium and Phomopsis with peroba rosa seeds. Floresta e Ambiente, 26, 1–7.
Mendes, S. S., Santos, P. R., Santana, G. C., Ribeiro, G. T., & Mesquita, J. B. (2005). Levantamento, patogenicidade e transmissão de fungos associados a sementes de sabiá (Mimosa caesalpiniaefolia). Revista Ciência Agronômica, 36, 118–122.
Nascimento, W. M. O., Cruz, E. D., Moraes, M. H. D., & Menten, J. O. M. (2006). Qualidade sanitária e germinação de sementes de Pterogyne nitens Tull. (Leguminosae - Caesalpinioideae). Revista Brasileira de Sementes, 28, 149–153.
Nelson, P. E., Toussoun, T. A., & Marasas, W. F. O. (1983). Fusarium species: An illustrated manual for identification. University Park: Pennsylvania State University Press.
O'Donnell, K., Kistler, H. C., Cigelnik, E., & Ploetz, R. C. (1998). Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences, 95, 2044–2049.
Oliveira, O. S. (1981). Fungos causadores de danos em Araucaria angustifolia (Bert.) O. Ktze. Floresta, 12, 23–27.
Phoulivong, S., Cai, L., Chen, H., McKenzie, E. H. C., Abdelsalam, K., Chukeatirote, E., & Hyde, K. D. (2010). Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Diversity, 44, 33–43.
Poletto, I., Muniz, M. F. B., Ceconi, D. E., Mezzomo, R., & Rodrigues, J. (2010). Influência da inoculação de Fusarium spp. e níveis de sombreamento no crescimento e desenvolvimento da erva-mate. Ciência Florestal, 20, 513–521.
Poletto, I., Lupatini, M., Muniz, M. F. B., & Antoniolli, Z. I. (2012). Caracterização e patogenicidade de isolados de Fusarium spp. causadores de podridão-de-raízes da erva-mate. Floresta, 42, 95–104.
Poletto, I., Muniz, M. F. B., Ceconi, D. E., & Poletto, T. (2015). Aspectos epidemiológicos da podridão-de-raízes da erva-mate (Ilex paraguariensis). Ciência Florestal, 25, 281–291.
Rizzini, C. T. (1990). Árvores e madeiras úteis do Brasil: Manual de dendrologia brasileira (304 p). São Paulo: Ed. Edgard Blücher Ltda.
Santos, A. F., Grigoletti Júnior, A., & Auer, C. G. (2000). Transmissão de fungos por sementes de espécies florestais. Floresta, 30, 119–128.
Santos, A. F., Parisi, J. J. D., & Menten, J. O. M. (2011). Patologia de Sementes Florestais. Colombo: Embrapa Florestas.
Santos, A. F., Eckstein, B., Muniz, M. F. B., Carmo, A. L. M., Auer, C. G., Jaccoud Filho, D. S., & Parisi, J. J. D. (2015). Patologia de sementes de espécies florestais no Brasil. Revisão Anual de Patologia de Plantas, 23, 193–211.
Singh, P. K., Khan, S. N., Harsh, N. S., & Pandey, R. (2001). Incidence of mycoflora and mycotoxins in some edible fruits and seeds of forest origin. Mycotoxin Research, 17, 46–58.
Summerell, B. A., Laurence, M. H., Liew, E. C. Y., & Leslie, J. F. (2010). Biogeography and phylogeography of Fusarium: A review. Fungal Diversity, 4, 3–13.
Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2006). MEGA 4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.
Veloso, H. P., Rangel Filho, A. L. R., & Lima, J. C. A. (1991). Classificação da vegetação brasileira, adaptada a um sistema universal. Rio de Janeiro: Fundação Instituto Brasileiro de Geografia e Estatística.
Ventura, J. A. (1999). Taxonomia de Fusarium e seus segredos. I – história, meios e procedimentos de cultivo. Revisão Anual de Patologia de Plantas. Passo Fundo, 7, 271–298.
Waalwijk, C., Koning, J. R. A., Baayen, R. P., & Gams, W. (1996). Discordant groupings of Fusarium spp. from sections Elegans, Liseola and Dlaminia based on ribossomal ITS1 and ITS2 sequences. Mycologia, 88, 361–368.
Walker, C., Maciel, C. G., Milanesi, P. M., Muniz, M. F. B., Mezzomo, R., & Pollet, C. S. (2016). Caracterização morfológica, molecular e patogenicidade de Fusarium acuminatum e Fusarium verticillioides a Cordia americana. Ciência Florestal, 26, 463–473.
Wingfield, M. J., Hammerbacher, A., Ganley, R. J., Steenkamp, E. T., Gordon, T. R., Wingfield, B. D., & Coutinho, T. A. (2008). Pitch canker caused by Fusarium circinatum: A growing threat to pine plantations and forests worldwide. Australasian Plant Pathology, 37, 319–334.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Shinsky, & T. J. White (Eds.), PCR Protocols: A Guide to Methods and Applications (pp. 315–322). San Diego: Academic Press.
Acknowledgments
The authors would like to thank the Academic Publishing Advisory Center (Centro de Assessoria de Publicação Acadêmica, CAPA - www.capa.ufpr.br) of the Federal University of Parana for assistance with English language editing.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that this paper has not been submitted to more than one journal for simultaneous consideration, has not been published previously partly or in full and no data have been fabricated or manipulated. There was no conflict of interest among authors and consent to submit has been received explicitly from all co-authors and the institute were the work has been carried out.
Rights and permissions
About this article
Cite this article
Mazarotto, E.J., Poitevin, C.G., do Carmo, A.L.M. et al. Pathogenic Fusarium species complexes associated to seeds of indigenous Brazilian forest tree Aspidosperma polyneuron. Eur J Plant Pathol 158, 849–857 (2020). https://doi.org/10.1007/s10658-020-02120-8
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-020-02120-8