A Fairy Chemical Suppresses Retinal Angiogenesis as a HIF Inhibitor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal
2.2. Cell Culture
2.3. Compounds from Mushroom-Forming Fungi
2.4. Luciferase Assay
2.5. Quantitative PCR
2.6. Western Blotting
2.7. Oxygen-Induced Retinopathy Model and Administration of a Compound from Mushrooms
2.8. Statistical Analysis
3. Results
3.1. AHX Showed an Inhibitory Effect on HIF Activity
3.2. Hypoxia-Responsive Gene Expressions Were Suppressed by AHX Treatment under CoCl2-Induced Hypoxic Condition
3.3. Neovascularization in a Murine OIR Model Was Suppressed by AHX Administration
4. Discussion
5. Conclusions
6. Patents
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
References
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Name | Direction | Sequence (5′ → 3′) | Accession Number |
---|---|---|---|
Hprt | Forward | TCAGTCAACGGGGGACATAAA | NM_013556.2 |
Reverse | GGGGCTGTACTGCTTAACCAG | ||
Hif-1α | Forward | GGTTCCAGCAGACCCAGTTA | NM_001313919.1 |
Reverse | AGGCTCCTTGGATGAGCTTT | ||
Hif-2α | Forward | CTGAGGAAGGAGAAATCCCGT | NM_010137.3 |
Reverse | TGTGTCCGAAGGAAGCTGATG | ||
Vegf | Forward | CCTGGTGGACATCTTCCAGGAGTACC | AY707864.1 |
Reverse | GAAGCTCATCTCTCCTATGTGCTGGC | ||
Bnip3 | Forward | GCTCCCAGACACCACAAGAT | NM_009760.4 |
Reverse | TGAGAGTAGCTGTGCGCTTC | ||
Pdk1 | Forward | GGCGGCTTTGTGATTTGTAT | NM_172665.5 |
Reverse | ACCTGAATCGGGGGATAAAC |
Name | 1st Trial in 3T3 | 2nd Trial in ARPE-19 | 3rd Trial in 661W | |||
---|---|---|---|---|---|---|
Fold Change ± SD | p-Value | Fold Change ± SD | p-Value | Fold Change ± SD | p-Value | |
Topotecan | 0.96 ± 0.26 | 0.855 | 0.84 ± 0.01 | 0.002 ** | 0.81 ± 0.11 | 0.074 |
Doxorubicin | 0.30 ± 0.06 | 0.014 * | 1.10 ± 0.09 | 0.135 | 0.71 ± 0.05 | 0.006 ** |
AHX | 0.39 ± 0.06 | 0.021 * | 0.39 ± 0.03 | <0.001 *** | 0.42 ± 0.07 | 0.001 ** |
AOH | 0.35 ± 0.03 | 0.016 * | 0.93 ± 0.04 | 0.091 | 0.85 ± 0.08 | 0.086 |
ICA | 1.02 ± 0.32 | 0.939 | ||||
Erinacine A | 1.50 ± 0.16 | 0.054 | ||||
Hericenone C | 1.25 ± 0.11 | 0.224 | ||||
Hericenone D | 0.79 ± 0.04 | 0.276 | ||||
Hericenone E | 0.67 ± 0.04 | 0.115 | ||||
Grifolin | 0.76 ± 0.06 | 0.223 | ||||
Negrifolin | 0.82 ± 0.03 | 0.338 |
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Lee, D.; Miwa, Y.; Wu, J.; Shoda, C.; Jeong, H.; Kawagishi, H.; Tsubota, K.; Kurihara, T. A Fairy Chemical Suppresses Retinal Angiogenesis as a HIF Inhibitor. Biomolecules 2020, 10, 1405. https://doi.org/10.3390/biom10101405
Lee D, Miwa Y, Wu J, Shoda C, Jeong H, Kawagishi H, Tsubota K, Kurihara T. A Fairy Chemical Suppresses Retinal Angiogenesis as a HIF Inhibitor. Biomolecules. 2020; 10(10):1405. https://doi.org/10.3390/biom10101405
Chicago/Turabian StyleLee, Deokho, Yukihiro Miwa, Jing Wu, Chiho Shoda, Heonuk Jeong, Hirokazu Kawagishi, Kazuo Tsubota, and Toshihide Kurihara. 2020. "A Fairy Chemical Suppresses Retinal Angiogenesis as a HIF Inhibitor" Biomolecules 10, no. 10: 1405. https://doi.org/10.3390/biom10101405