Abstract
Bread wheat is a widely cultivated crop with production that is often negatively influenced by disease outbreaks. Biological constraints in production include the wheat rusts (leaf rust, stem rust and stripe rust) and Fusarium head blight (FHB). Different strategies are available in the control of these important fungal diseases. However, resistance breeding remains the preferred option of control as it lowers the risk of disease outbreaks and is a more cost effective and environmentally friendly approach. Many effective rust and FHB resistance genes have been discovered and successfully deployed through resistance breeding worldwide. However, some of these genes, when deployed singly, are vulnerable to evolving pathogens. In this study we aimed to develop wheat lines with the prospect of durable resistance against the rusts and FHB through combining eight resistance genes/quantitative trait loci; Lr19, Lr34/Yr18/Sr57/Pm38/Ltn1, Sr2/Yr30, Sr26, Sr39, Fhb1, Qfhs.ifa-5A-1 and Qfhs.ifa-5A-2 into a single wheat plant using marker-assisted selection. Cross and self-pollinated populations were developed to increase the frequency and homozygosity levels of resistance genes in progeny. Molecular markers were furthermore applied to determine the identity of the high molecular weight-glutenin subunits (HMW-GS) and to screen for the presence of the 1BL.1RS translocation in the final populations. We were successful in developing wheat plants containing complex sources of rust and FHB resistance and confirmed the presence of HMW-GS markers linked to strong dough strength and good bread making qualities in these genotypes.
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References
Agenbag GM, Pretorius ZA, Boyd LA, Bender CM, MacCormack R, Prins R (2014) High-resolution mapping and new marker development for adult plant stripe rust resistance QTL in the wheat cultivar Kariega. Mol Breed 34:2005–2020. https://doi.org/10.1007/s11032-014-0158-4
Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, Moreno-Sevilla B, Fetch JM, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theor Appl Genet 102:1164–1168. https://doi.org/10.1007/s001220000509
Bakhsh A, Mengistu N, Baenziger PS, Dweikat I, Wegulo SN, Rose DJ, Bai G, Eskridge KM (2013) Effect of Fusarium head blight resistance gene Fhb1 on agronomic and end-use quality traits of hard red winter wheat. Crop Sci 53:793–801. https://doi.org/10.2135/cropsci2012.06.0364
Bita CE, Gerats T (2013) Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops. Front Plant Sci 273:1–18. https://doi.org/10.3389/fpls.2013.00273
Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508. https://doi.org/10.1007/s00122-003-1272-6
Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26. https://doi.org/10.1111/j.1439-0523.2008.01550.x
Butow BJ, Ma W, Gale KR, Cornish GB, Rampling L, Larroque O, Morell MK, Békés F (2003) Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high-molecular-weight glutenin allele has a major impact on wheat flour dough strength. Theor Appl Genet 107:1524–1532. https://doi.org/10.1007/s00122-003-1396-8
Butow BJ, Gale KR, Ikea J, Juhasz A, Bedo Z, Tamas L, Gianibelli MC (2004) Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor Appl Genet 109:1525–1535. https://doi.org/10.1007/s00122-004-1776-8
Deng ZY, Tian JC, Liu XP (2004) Accumulation regularity of protein components in wheat cultivars with different HMW-GS. Acta Agronom Sin 30:481–486. https://doi.org/10.1007/s11703-009-0046-6
Dexter E, Clear RM, Preston KR (1996) Fusarium head blight: effect on the milling and baking of some Canadian wheats. Cereal Chem 73:695–701
FAO (2020) Cereal supply and demand brief. http://www.fao.org/worldfoodsituation/csdb/en/. Accessed 17 Apr 2020
Francis HA, Leitch AR, Koebner RMD (1995) Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theor Appl Genet 90:636–642. https://doi.org/10.1007/BF00222127
Gold J, Harder D, Townley-Smith F, Aung T, Procunier J (1999) Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. Electron J Biotechnol 2:1. https://doi.org/10.2225/vol2-issue1-fulltext-1
Halford NG, Field JM, Blair H, Urwin P, Moore K, Robert L, Thompson R, Flavell RB, Tatham AS, Shewry PR (1992) Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83:373–378. https://doi.org/10.1007/BF00224285
Jia H, Zhou J, Xue S, Li G, Yan H, Ran C, Zhang Y, Shi J, Jia L, Wang X, Luo J, Ma Z (2018) A journey to understand wheat Fusarium head blight resistance in the Chinese wheat landrace Wangshuibai. Crop J 6:48–59. https://doi.org/10.1016/j.cj.2017.09.006
Juroszek P, von Tiedemann A (2011) Potential strategies and future requirements for plant disease management under a changing climate. Plant Pathol 60:100–112. https://doi.org/10.1111/j.1365-3059.2010.02410.x
Kerber ER, Dyck PL (1990) Transfer to hexaploid wheat of linked genes for adult-plant leaf rust and seedling stem rust resistance from an amphiploid of Aegilops speltoides x Triticum monococcum. Genome 33:530–537. https://doi.org/10.1139/g90-079
Knott DR (1961) The inheritance of rust resistance VI. The transfer of stem rust resistance from Agropyron elongatum to common wheat. Can J Plant Sci 41:109–123. https://doi.org/10.4141/cjps61-014
Kolmer JA, Mert Z, Akan K, Demir L, Unsal R, Sermet C, Keser M, Akin B, Morgounov A (2013) Virulence of Puccinia triticina in Turkey and leaf rust resistance in Turkish wheat cultivars. Eur J Plant Pathol 135:703–716. https://doi.org/10.1007/s10658-012-0107-1
Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898. https://doi.org/10.1007/s00122-009-1097-z
Liang D, Tang J, Peña RJ, Singh R, He X, Shen X, Yoa D, Xia X, He Z (2010) Characterization of CIMMYT bread wheats for high and low-molecular weight glutenin subunits and other quality-related genes with SDS-PAGE, RP-HPLC and molecular markers. Euphytica 172:235–250. https://doi.org/10.1007/s10681-009-0054-x
Liu S, Chao S, Anderson JA (2008a) New DNA markers for high molecular weight glutenin subunits in wheat. Theor Appl Genet 118:177–183. https://doi.org/10.1007/s00122-008-0886-0
Liu S, Pumphrey MO, Gill BS, Trick HN, Zhang JX, Dolezel J, Chalhoub B, Anderson JA (2008b) Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Res Commun 36:195–201. https://doi.org/10.1556/CRC.36.2008.Suppl.B.15
Liu S, Yu L, Singh RP, Jin Y, Sorrells ME, Anderson JA (2010) Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26. Theor Appl Genet 120:691–697. https://doi.org/10.1007/s00122-009-1186-z
Mago R, Bariana HS, Dundas IS, Spielmeyer W, Lawrence GJ, Pryor AJ, Ellis JG (2005) Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor Appl Genet 111:496–504. https://doi.org/10.1007/s00122-005-2039-z
Mago R, Zhang P, Bariana HS, Verlin DC, Bansal UK, Ellis JG, Dundas IS (2009) Development of wheat lines carrying stem rust resistance gene Sr39 with reduced Aegilops speltoides chromatin and simple PCR markers for marker-assisted selection. Theor Appl Genet 199:1441–1450. https://doi.org/10.1007/s00122-009-1146-7
Mago R, Brown-Guedira G, Dreisigacker S, Breen J, Jin Y, Singh R, Appels R, Lagudah ES, Ellis J, Spielmeyer W (2011) An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat. Theor Appl Genet 122:735–744. https://doi.org/10.1007/s00122-010-1482-7
Marchal C, Zhang J, Zhang P, Fenwick P, Steuernagel B, Adamski NM, Boyd L, McIntosh R, Wulff BBH, Berry S, Lagudah E, Uauy C (2018) BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust. Nat Plants 4:662–668. https://doi.org/10.1038/s41477-018-0236-4
Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386. https://doi.org/10.1111/j.1439-0523.1995.tb00816.x
Miedaner T, Wilde F, Steiner B, Buersmayr H, Korzun V, Ebmeyer E (2006) Stacking quantitative trait loci (QTL) for Fusarium head blight resistance from non-adapted sources in an European elite spring wheat background and assessing their effects on deoxynivalenol (DON) content and disease severity. Theor Appl Genet 112:562–569
Minnaar-Ontong A, Herselman L, Kriel WM, Leslie JF (2017) Morphological characterization and trichothecene genotype analysis of a Fusarium head blight population in South Africa. Eur J Plant Pathol 148:261–269. https://doi.org/10.1007/s10658-016-1085-5
Moloi MJ, vanBiljon A, Labuschagne MT (2017) Effect of quantity of HMW-GS 1Ax1, 1Bx13, 1By16, 1Dx5 and 1Dy10 on baking quality in different genetic backgrounds and environments. LWT Food Sci Technol 78:160–164. https://doi.org/10.1016/j.lwt.2016.12.031
Payne PI, CorWeld KG, Holt LM, Blackman JA (1981) Correlations between the inheritance of certain high molecular weight subunits of glutenin and bread-making quality in progenies of 6 crosses of bread wheat. J Sci Food Agr 32:51–60. https://doi.org/10.1002/jsfa.2740320109
Popineau Y, Cornec M, Lefebvre J, Marchylo B (1994) Influence of high Mr glutenin subunits on glutenin polymers and rheological properties of glutens and gluten subfractions of near-isogenic lines of wheat Sicco. J Cereal Sci 19:231–241. https://doi.org/10.1006/jcrs.1994.1030
Pretorius ZA, Prins R, Wessels E, Bender CM, Visser B, Boshoff WHP (2020) Accomplishments in wheat rust research in South Africa. SA J Sci 297:537–541
Prins R, Groenewald JZ, Marais GF, Snape JW (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet 103:618–624. https://doi.org/10.1007/PL00002918
Priyamvada A, Saharan MS, Tiwari R (2011) Durable resistance in wheat. Int J Genet Mol Biol 3:108–114
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023. https://doi.org/10.1023/A:1017510331721
Rutkoski JE, Heffner EL, Sorrells ME (2011) Genomic selection for durable stem rust resistance in wheat. Euphytica 179:161–173. https://doi.org/10.1007/s10681-010-0301-1
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018. https://doi.org/10.1073/pnas.81.24.8014
Salameh A, Buerstmayr M, Steiner B, Neumayer A, Lemmens M, Buerstmayr H (2011) Effects of introgression of two QTL for Fusarium head blight resistance from Asian spring wheat by marker-assisted backcrossing into European winter wheat on Fusarium head blight resistance, yield and quality traits. Mol Breed 28:485–494. https://doi.org/10.1007/s11032-010-9498-x
Sharma D, Knott DR (1966) The transfer of leaf rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol 8:137–143
Singh RP, Huerta-Espino J (1997) Effect of leaf rust gene Lr34 on grain yield and agronomic traits of spring wheat. Crop Sci 37:390–395. https://doi.org/10.2135/cropsci1997.0011183X003700020014x
Singh RP, Rajaram S, Crossa J (1998) Agronomic effects from chromosome translocations 7DL.7Ag and 1BL.1RS in spring wheat. Crop Sci 38:27–33. https://doi.org/10.2135/cropsci1998.0011183X003800010005x
Singh RP, Herrera-Foessel S, Huerta-Espino J, Singh S, Bhavani S, Lan C, Basnet BR (2014) Progress towards genetics and breeding for minor genes based resistance to Ug99 and other rusts in CIMMYT high-yielding spring wheat. J Integr Agric 13:255–261. https://doi.org/10.1016/S2095-3119(13)60649-8
Smit HA, Tolmay VL, Barnard A, Jordaan JP, Koekemoer FP, Otto WM, Pretorius ZA, Purchase JL, Tolmay JPC (2010) An overview of the context and scope of wheat (Triticum aestivum) research in South Africa from 1983 to 2008. S Afr J Plant Soil 27:81–96. https://doi.org/10.1080/02571862.2010.10639973
Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336. https://doi.org/10.2135/cropsci2003.3330
Steiner B, Buerstmayr M, Wagner C, Danler A, Eshonkulov B, Ehn M, Buerstmayr H (2019) Fine-mapping of the Fusarium head blight resistance QTL Qfhs.ifa-5A identifies two resistance QTL associated with anther extrusion. Theor Appl Genet 132:2039–2053. https://doi.org/10.1007/s00122-019-03336-x
Sydenham SL (2014) Marker-assisted backcross breeding for Fusarium head blight resistance in South African wheat. Dissertation, University of the Free State
Tamburic-Ilincic L (2012) Effect of 3B, 5A and 3A QTL for Fusarium head blight resistance on agronomic and quality performance of Canadian winter wheat. Plant Breed 131:722–727. https://doi.org/10.1111/pbr.12005
Van Coller GJ (2018) Identification and management of toxigenic Fusarium species associated with Fusarium head blight and crown rot in South Africa. Dissertation, University of Stellenbosch
Vawser MJ, Cornish GB (2004) Over-expression of HMW glutenin subunit Glu-Bx17 in hexaploid wheat varieties (Triticum aestivum). Aust J Agric Res 55:577–588. https://doi.org/10.1071/AR03227
Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811. https://doi.org/10.2135/cropsci1999.0011183X003900030032x
Wellings CR, Singh RP, Yahyaoui A, Nazari K, McIntosh RA (2009) The development and application of near-isogenic lines for monitoring cereal rust pathogens. In: McIntosh RA (ed) Borlaug global rust initiative technical workshop. BGRI Cd Obregon, Mexico, pp 77–87
Winter P, Kahl G (1995) Molecular marker technologies for plant improvement. World J Microbiol Biotechnol 11:438–448. https://doi.org/10.1007/BF00364619
Xia C, Wang M, Yin C, Cornejo OE, Hulbert SH, Chen X (2018) Genomic insights into host adaptation between the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei). BMC Genom 19:664. https://doi.org/10.1186/s12864-018-5041-y
Xue S, Xu F, Tang M, Zhou Y, Li G, An X, Lin F, Xu H, Jia H, Zhang L, Kong Z, Ma Z (2011) Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 123:1055–1063. https://doi.org/10.1007/s00122-011-1647-z
Zhang X, Rouse MN, Nava IC, Jin Y, Anderson JA (2016) Development and verification of wheat germplasm containing both Sr2 and Fhb1. Mol Breed 36:85. https://doi.org/10.1007/s11032-016-0502-y
Zhu X, Li Z, Xu H, Zhou M, Du L, Zhang Z (2012) Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat. Funct Integr Genom 12:481–488. https://doi.org/10.1007/s10142-012-0286-z
Zhu Z, Hao Y, Mergoum M, Bai G, Humphreys G, Cloutier S, Xia X, He Z (2019) Breeding wheat resistance for Fusarium head blight in the Global North: China, USA, and Canada. Crop J 7:730–738. https://doi.org/10.1016/j.cj.2019.06.003
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This study was partly funded by the Winter Cereal Trust (WCT/W/2006/02) and the National Research Foundation (SARChI chair UID 8464), South Africa.
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Maré, A., Boshoff, W.H.P. & Herselman, L. Molecular breeding of wheat lines for multiple rust and Fusarium head blight resistance. Euphytica 216, 163 (2020). https://doi.org/10.1007/s10681-020-02697-5
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DOI: https://doi.org/10.1007/s10681-020-02697-5