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Genetic Diversity Assessment of a Chinese Medicinal Endemic Species, Aspidopterys obcordata var. obcordata, by Combined Molecular Marker Methods (ISSR & SRAP)

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Abstract

Aspidopterys obcordata var. obcordata, a medicinal plant endemic to China, is a narrowly distributed species and wild resources are extremely limited. To evaluate the genetic variability and degree of genetic divergence of A. obcordata var. obcordata, and to make rational scientific decisions on its harvest and germplasm conservation, we collected 122 samples from across nearly all of its distribution area and studied genetic diversity using inter-simple sequence repeats (ISSRs), sequence-related amplified polymorphisms (SRAPs), and a method combining the two techniques. The results revealed the high genetic diversity of A. obcordata var. obcordata, mainly due to its intra-population diversity, and the top two populations with the highest levels of intra-population diversity were ML and DH, individuals of which can serve as excellent germplasm candidates during the processing of germplasm screening and conservation. In general, the combining method was prior to the ISSR analyses and SRAP analyses results, except for a slight difference in the genetic structure of individual populations. Therefore, we suggest that a combination analysis of the two marker methods is ideal for evaluating the genetic diversity and genetic relationships of A. obcordata var. obcordata.

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Abbreviations

ISSR:

Inter-simple sequence repeat

SRAPs:

Sequence-related amplified polymorphisms

UPGMA:

Unweighted pair group method with arithmetic means

AMOVA:

Analyses of molecular variance

PCA:

Principal component analyses

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Funding

This research was funded by CAMS Initiative for Innovative Medicine (Grant No. 2017-12M-B&R-09) and Special subsidies for public health service of TCM "the national survey of TCM resources" (Grant No. DSS, MOF. No 66/2017).

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Author notes

  1. Li-Xia Zhang and Zhong-Lian Zhang have contributed equally to this work.

Authors and Affiliations

  1. The Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, No. 138 Xuanwei Avenue, Jinghong, 666100, Yunnan, China

    Mei-Fang Song, Yan-Hong Guan, Yue Zhang, Hai-Tao Li, Li-Xia Zhang & Zhong-Lian Zhang

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  1. Mei-Fang Song
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  2. Yan-Hong Guan
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  5. Li-Xia Zhang
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Contributions

L-XZ fund this study. Z-LZ designed the experiment, collected plant material, and analyzed experimental data. M-FS performed the experiment, analyzed experimental data, and drafted the manuscript. Y-HG and H-TL collected plant material. YZ provided experimental help. The authors are accountable for all aspects of the work.

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Correspondence to Li-Xia Zhang or Zhong-Lian Zhang.

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Song, MF., Guan, YH., Zhang, Y. et al. Genetic Diversity Assessment of a Chinese Medicinal Endemic Species, Aspidopterys obcordata var. obcordata, by Combined Molecular Marker Methods (ISSR & SRAP). Biochem Genet 59, 283–299 (2021). https://doi.org/10.1007/s10528-020-10001-2

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