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A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

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Abstract

A novel sensing chip was designed for MALDI-MS quantitation of acid phosphatase (ACP). The ACP sensing chip was constructed through non-covalent interaction of streptavidin and biotin for the assembly of biotinylated peptide substrate on biotinylated polyethylene-glycol (PEG) modified indium-tin oxide (ITO) slide. In the presence of ACP, the peptide substrate was dephosphorylated under acidic condition to generate a new mass signal. The quantitative assay of ACP was achieved with the mass signal ratio of product to the sum of product and left peptide substrate. Under optimal detection conditions, the ratio was linearly correlated with the concentration of ACP in the range of 0.05–12 g/L with a detection limit (LOD) of 0.04 g/L. The designed ACP sensing chip has been used to analyze ACP in complex clinical samples, which exhibited high selectivity, good repeatability, and admirably anti-interference ability. This work further demonstrates the concept of MS sensing and the application of MALDI-MS in quantitative analysis, and provides a convenient method for the quantitation of proteases in clinical diagnosis.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21635005, 21827812, 21890741, 21974063), the National Key Research and Development Program of China (2018YFC1004704) and the Fundamental Research Funds for the Central Universities (14380200).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China

    Qiulin Ma, Yunlong Chen, Nan Feng & Huangxian Ju

  2. Department Clinical Laboratory, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing, 210009, China

    Feng Yan

Authors
  1. Qiulin Ma
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  2. Yunlong Chen
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  3. Nan Feng
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  4. Feng Yan
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  5. Huangxian Ju
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Corresponding author

Correspondence to Huangxian Ju.

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The authors declare no conflict of interest.

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Ma, Q., Chen, Y., Feng, N. et al. A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase. Sci. China Chem. 64, 151–156 (2021). https://doi.org/10.1007/s11426-020-9850-3

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  • DOI: https://doi.org/10.1007/s11426-020-9850-3

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