Abstract
Aedes aegypti is a globally spread disease vector of supreme concern, primarily controlled by chemical insecticides. Current study investigates the comparative rate of acetamiprid and deltamethrin resistance development in Ae. aegypti larvae and; possible correlation between resistance and cuticular thickening. The larvae were selected with LC90 level of the respective insecticide for 10 successive generations and the level of resistance induced was estimated. The larvae of parent (PS), acetamiprid-selected (ACSF-10) and deltamethrin-selected (DLSF-10) strains were sectioned through first abdominal segment to elucidate the variation in cuticular thickness. The PS larvae of Ae. aegypti were 229.26-fold higher susceptible to deltamethrin as compared to acetamiprid, exhibiting corresponding LC50 values of 0.00082 mg/L and 0.18799 mg/L. Larval selections induced 9.91-fold and 19.74-fold resistance to deltamethrin and acetamiprid, respectively; indicating multifactorial and heterozygous pattern. Cuticular analysis of the larval sections demonstrated a significantly thicker cuticle in selected strains, the mean thickness in PS, ACSF-10 and DLSF-10 was 2.891 ± 0.243, 4.288 ± 0.508 and 5.695 ± 0.437 μm, respectively revealing 1.48-fold and 1.97-fold thicker cuticle in the ACSF-10 and DLSF-10 strains, respectively in comparison to PS strain. The increased cuticular thickness possibly inhibited the insecticide penetration in selected larvae resulting in the resistance development. This is the first such report which demonstrates the association between acetamiprid/deltamethrin resistance in Ae. aegypti larvae with the cuticular thickness.
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Acknowledgements
The authors are highly grateful to the Council of Scientific Research (CSIR), New Delhi, India for providing financial assistance to carry out the present investigations. We are also highly grateful to the Principal, Acharya Narendra Dev College for providing infrastructure and research facilities.
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This research was supported by the contingent grant from Council of Scientific and Industrial Research, New Delhi, India (Award No. 08/529(0003)/2015-EMR-I).
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RRS conceived the idea, conducted the experiments and wrote the manuscript. SK designed and guided the experiments. RRS analysed the results and SK helped in the statistical analysis. Both the authors were involved in the finalization of manuscript.
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Samal, R.R., Kumar, S. Cuticular thickening associated with insecticide resistance in dengue vector, Aedes aegypti L.. Int J Trop Insect Sci 41, 809–820 (2021). https://doi.org/10.1007/s42690-020-00271-z
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DOI: https://doi.org/10.1007/s42690-020-00271-z