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Antimicrobial Activity of a Novel Freshwater Planctomycete Lacipirellula parvula PX69T

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Abstract—

Planctomycetes are a phylogenetic group of bacteria with complex cell organization, large genomes and a wide spectrum of genome-encoded secondary metabolites. As suggested by analysis of available planctomycete genomes, these bacteria represent a promising source of potentially novel biologically active compounds. The number of cultured planctomycetes, however, remains limited. This study was undertaken in order to assess the presence of antimicrobial activity and its spectrum in the recently described freshwater planctomycete Lacipirellula parvula PX69T representing the novel family Lacipirellulaceae. The genome of strain PX69T contained 6 gene clusters encoding type III polyketide synthases, non-ribosomal peptide synthetases, and other secondary metabolites, which displayed low similarity to those in other bacteria. Strain PX69T was shown to exhibit antimicrobial activity against a number of test microorganisms. The highest antimicrobial activity, up to 83‒87% of growth inhibition, was observed against Staphylococcus aureus and Candida albicans. The medium composition and the cultivation time have been optimized in order to maximize antimicrobial activity of strain PX69T.

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Funding

The work was supported by the Russian Science Foundation, project no. 16-14-10210.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    S. E. Belova, V. A. Saltykova & S. N. Dedysh

  2. Moscow State University, 119991, Moscow, Russia

    V. A. Saltykova

Authors
  1. S. E. Belova
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  2. V. A. Saltykova
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  3. S. N. Dedysh
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Contributions

The idea and scheme of the work were proposed by SD. Genome analysis for strain PX69Т was carried out by VS. Experiments on planctomycete cultivation, extraction of its secondary metabolites, and determination of their antimicrobial activity were carried out by SB and VS. SB and SD wrote the manuscript. All authors discussed the experimental results.

Corresponding author

Correspondence to S. N. Dedysh.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by P. Sigalevich

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Belova, S.E., Saltykova, V.A. & Dedysh, S.N. Antimicrobial Activity of a Novel Freshwater Planctomycete Lacipirellula parvula PX69T . Microbiology 89, 503–509 (2020). https://doi.org/10.1134/S0026261720050045

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  • DOI: https://doi.org/10.1134/S0026261720050045

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