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Establishment and characterization of a cell line from Ghanaian Aedes aegypti (Diptera: Culicidae) focusing on Aedes-borne flavivirus susceptibility

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Abstract

Mosquitoes are generally considered one of the most important vectors of arboviruses, with Aedes aegypti regarded as the most important in transmission of yellow fever and dengue viruses. To investigate why there are differences in the incidence of dengue fever and Zika in different geographical areas and an absence of outbreaks in Ghana in spite of an abundance of A. aegypti mosquitoes, we established a continuous cell line from embryonic cells of A. aegypti collected in Ghana and assessed its susceptibility to dengue, yellow fever, and Zika viruses. The new cell line (designated AeAe-GH98), having an adhesive spindle-shaped web-like morphology, was serially subcultured in both VP-12 and Schneider’s medium supplemented with 10% heat-inactivated fetal bovine serum. AeAe-GH98 cells were found to have a population doubling time of 1.3 d during exponential growth. The mosquito colony used to establish the cell line was confirmed to have originated from Africa using microsatellite assay. In terms of susceptibility to Aedes-borne flaviviruses, AeAe-GH98 cells were found to have different degrees of susceptibility to yellow fever, Zika, and dengue virus infection and propagation. While susceptibility of AeAe-GH98 cells to yellow fever and Zika viruses was comparable with that of C6/36 cells, susceptibility to dengue virus was significantly lower. This cell line will serve as a useful tool for determining molecular factors influencing virus–vector susceptibility in vitro.

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Funding

This study was funded by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) and the Research Program on Emerging and Re-emerging Infectious Diseases from the Japan Agency for Medical Research and Development (AMED). This study was also supported in part by JSPS KAKENHI Grant Numbers JP16J09470, JP15H04614, and JP18H02856 and by the Sasakawa Scientific Research Grant from The Japan Science Society.

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Authors and Affiliations

  1. Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

    Michael Amoa-Bosompem, Daisuke Kobayashi, Astri Nur Faizah, Kyoko Sawabe & Haruhiko Isawa

  2. Department of Environmental Parasitology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-850, Japan

    Michael Amoa-Bosompem & Shiroh Iwanaga

  3. Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, P.O.Box LG581, Legon, Accra, Ghana

    Michael Amoa-Bosompem & Samuel Dadzie

  4. Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

    Kentaro Itokawa

  5. Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Astri Nur Faizah

  6. Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari, Ehime, Japan

    Ryusei Kuwata

  7. Department of Molecular Virology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-850, Japan

    Takaya Hayashi & Shoji Yamaoka

Authors
  1. Michael Amoa-Bosompem
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  2. Daisuke Kobayashi
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  3. Kentaro Itokawa
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  4. Astri Nur Faizah
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  5. Ryusei Kuwata
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  6. Samuel Dadzie
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  7. Takaya Hayashi
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  8. Shoji Yamaoka
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  9. Kyoko Sawabe
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  10. Shiroh Iwanaga
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  11. Haruhiko Isawa
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Corresponding author

Correspondence to Haruhiko Isawa.

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Editor: Tetsuji Okamoto

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Amoa-Bosompem, M., Kobayashi, D., Itokawa, K. et al. Establishment and characterization of a cell line from Ghanaian Aedes aegypti (Diptera: Culicidae) focusing on Aedes-borne flavivirus susceptibility. In Vitro Cell.Dev.Biol.-Animal 56, 792–798 (2020). https://doi.org/10.1007/s11626-020-00504-y

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