This month’s Under the Lens discusses recent advances in high-resolution imaging of bacterial membrane proteins within intact liposomes by electron cryomicroscopy.
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References
Yao, X. et al. Cryo-EM analysis of a membrane protein embedded in the liposome. Proc. Natl Acad. Sci. USA 117, 18497–18503 (2020).
Liu, Y. et al. Automatic cryo-EM particle selection for membrane proteins in spherical liposomes. J. Struct. Biol. 185, 295–302 (2014).
Han, Y. et al. High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy. Proc. Natl Acad. Sci. USA 117, 1009–1014 (2020).
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Hankins, M.T.K., Bublitz, M. Observing bacterial membrane proteins in their quasi-natural habitat. Nat Rev Microbiol 18, 676 (2020). https://doi.org/10.1038/s41579-020-00463-x
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DOI: https://doi.org/10.1038/s41579-020-00463-x