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Peculiarities of the Expression of Inducible NO Synthase in Rat Dentate Gyrus in Depression Modeling

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Mild stress exposure contributes to the development of cognitive and emotional deficits, is considered as a model of depressive state, and is characterized by enhanced NO production. In albino mature (12-month-old) male rats, the depressive state was simulated by daily 30-min exposure to stressful stimuli (vibration, loud sound, and strobe light) over 7 days in a special chamber. On paraffin frontal sections of the brain stained with antibodies against inducible NO synthase (iNOS), the expression and distribution pattern of immunoreactive material were evaluated in various layers of the dentate gyrus under normal conditions and after depression modeling. The relative area of iNOS expression in the dentate gyrus of control rats was 8.2 (7.1-9.9)%, while in rats with experimental depression, this parameter was 16.7 (10.5-22.1)%, i.e. increased by 8.5% (p<0.05). In mature rats with modeled depressive state, the expression and relative area of iNOS expression in neuronal perikarya in the granular and subgranular layers of the dentate gyrus increased, which can underlie the mechanisms of damage and determine reduced neuroplasticity and suppressed neurogenesis in the dentate gyrus in rats during adulthood.

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References

  1. Alves ND, Patrício P, Correia JS, Mateus-Pinheiro A, Machado-Santos AR, Loureiro-Campos E, Morais M, Bessa JM, Sousa N, Pinto L. Chronic stress targets adult neurogenesis preferentially in the suprapyramidal blade of the rat dorsal dentate gyrus. Brain Struct. Funct. 2018;223(1):415-428.

    PubMed  Google Scholar 

  2. Bessa JM, Ferreira D, Melo I, Marques F, Cerqueira JJ, Palha JA, Almeida OF, Sousa N. The mood-improving actions of antidepressants do not depend on neurogenesis but are associated with neuronal remodeling. Mol. Psychiatry. 2009;14(8):764-773.

  3. Ogłodek EA, Just MJ. The association between inflammatory markers (iNOS, HO-1, IL-33, MIP-1β) and depression with and without posttraumatic stress disorder. Pharmacol. Rep. 2018;70(6):1065-1072.

    PubMed  Google Scholar 

  4. Pascual M, Blanco AM, Cauli O, Miñarro J, Guerri C. Intermittent ethanol exposure induces inflammatory brain damage and causes long-term behavioural alterations in adolescent rats. Eur. J. Neurosci. 2007;25(2):541-550.

    PubMed  Google Scholar 

  5. Patrício P, Mateus-Pinheiro A, Irmler M, Alves ND, Machado-Santos AR, Morais M, Correia JS, Korostynski M, Piechota M, Stoffel R, Beckers J, Bessa JM, Almeida OF, Sousa N, Pinto L. Differential and converging molecular mechanisms of antidepressants’ action in the hippocampal dentate gyrus. Neuropsy-chopharmacology. 2015;40(2):338-349.

    Google Scholar 

  6. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. New York, 2007.

  7. Sahay A, Hen R. Adult hippocampal neurogenesis in depression. Nat. Neurosci. 2007;10(9):1110-1115.

    CAS  PubMed  Google Scholar 

  8. Smirnov AV, Tyurenkov IN, Shmidt MV, Ekova MR, Mednikov DS, Borodin DD. Effect of combined stress on morphological changes and expression of NO synthases in rat ventral hippocampus. Bull. Exp. Biol. Med. 2015;160(1):96-99.

    CAS  PubMed  Google Scholar 

  9. Snyder JS, Soumier A, Brewer M, Pickel J, Cameron HA. Adult hippocampal neurogenesis buffers stress responses and depressive behavior. Nature. 2011;476:458-461.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Talarowska M, Szemraj J, Berk M, Maes M, Gałecki P. Oxidant/antioxidant imbalance is an inherent feature of depression. BMC Psychiatry. 2015;15. ID 71. doi: https://doi.org/10.1186/s12888-015-0454-5

  11. Tyurenkov IN, Bagmetova VV, Chernysheva YV, Borodin DD. A depressive state in rats in chronic combined stress induced by combined stresses of different modalities. Neurosci. Behav. Physiol. 2015;45(5):542-549.

    Google Scholar 

  12. Willner P, Scheel-Krüger J, Belzung C. The neurobiology of depression and antidepressant action. Neurosci. Biobehav. Rev. 2013;37(10, Pt 1):2331-2371.

    CAS  PubMed  Google Scholar 

  13. Zhou XY, Zhang F, Ying CJ, Chen J, Chen L, Dong J, Shi Y, Tang M, Hu XT, Pan Z.H, Xu NN, Zheng KY, Tang RX, Song YJ. Inhibition of iNOS alleviates cognitive deficits and depression in diabetic mice through downregulating the NO/sGC/cGMP/PKG signal pathway. Behav. Brain Res. 2017;322(Pt A):70-82.

    CAS  PubMed  Google Scholar 

  14. Zhu L, Bi W, Lu D, Zhang C, Shu X, Wang H, Qi R, Shi Q, Lu D. Regulation of ubiquitin-specific processing protease 8 suppresses neuroinflammation. Mol. Cell. Neurosci. 2015;64:74-83.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Volgograd State Medical University, the Ministry of Health of Russian Federation, Volgograd, Russia

    M. R. Ekova, A. V. Smirnov, I. N. Tyurenkov & N. V. Grigor’eva

  2. Volgograd Medical Research Center, Volgograd, Russia

    A. V. Smirnov

Authors
  1. M. R. Ekova
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  2. A. V. Smirnov
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  3. I. N. Tyurenkov
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  4. N. V. Grigor’eva
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Correspondence to M. R. Ekova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 653-656, May, 2020

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Ekova, M.R., Smirnov, A.V., Tyurenkov, I.N. et al. Peculiarities of the Expression of Inducible NO Synthase in Rat Dentate Gyrus in Depression Modeling. Bull Exp Biol Med 169, 718–720 (2020). https://doi.org/10.1007/s10517-020-04963-x

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  • DOI: https://doi.org/10.1007/s10517-020-04963-x

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